PMID- 36097121
OWN - NLM
STAT- MEDLINE
DCOM- 20221101
LR  - 20221101
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 49
IP  - 11
DP  - 2022 Nov
TI  - The association between several autophagy-related genes and their prognostic 
      values in hepatocellular carcinoma: a study on the foundation of TCGA, GEPIA and 
      HPA databases.
PG  - 10269-10277
LID - 10.1007/s11033-022-07426-w [doi]
AB  - BACKGROUND: The purpose of this study was to investigate the relationship between 
      the expression of autophagy-related genes and prognosis in hepatocellular 
      carcinoma (HCC). METHODS AND RESULTS: We selected three autophagy-related genes 
      (ATG3, ATG7, and ATG9A) from gene expression data of liver cancer patients in The 
      Cancer Genome Atlas (TCGA) database by Kaplan-Meier survival analysis, univariate 
      and multivariate Cox regression analysis, and Gene Set Enrichment Analysis 
      (GSEA). Human Protein Atlas (HPA) and Gene Expression Profiling Interactive 
      Analysis (GEPIA) databases were applied to testify the credibility of our 
      results. The expression levels of ATG3, ATG7, and ATG9A were verified by 
      real-time quantitative PCR (RT-qPCR) in normal liver cells (L02) and three HCC 
      cell lines (HepG2, Hep3b, and Li-7). Data analysis results from TCGA showed high 
      ATG3, ATG7, ATG9A expression in HCC tumor tissues. Kaplan-Meier survival analysis 
      showed that the survival rate of the high expression group of ATG3, ATG7, and 
      ATG9A was all significantly lower than the low expression group. GSEA analysis 
      showed that many signaling pathways (such as the regulation of autophagy, glycine 
      serine and threonine metabolism, pathways in cancer, mitogen-activated protein 
      kinase (MAPK) signaling pathway, mammalian target of rapamycin (mTOR) signaling 
      pathway, as well as P53 signaling pathway) were differentially enriched in HCCs 
      with ATG3, ATG7, and ATG9A expression. GEPIA and RT-qPCR also identified that the 
      mRNA expression level of ATG3, ATG7, and ATG9A in normal liver cells were 
      significantly lower than in HCC cells. High protein expression of ATG3, ATG7, and 
      ATG9A was displayed in HCCs from the HPA database. CONCLUSIONS: The ATG3, ATG7, 
      ATG9A might be utilized as prognostic biomarkers for liver cancer.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Zhao, Xueying
AU  - Zhao X
AD  - College of Biological Sciences and Biotechnology, Beijing Forestry University, 
      100083, Beijing, China.
FAU - Yin, Shangqi
AU  - Yin S
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - Shi, Jingren
AU  - Shi J
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - Zheng, Mei
AU  - Zheng M
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - He, Chaonan
AU  - He C
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - Meng, Huan
AU  - Meng H
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - Han, Ying
AU  - Han Y
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - Chen, Jin
AU  - Chen J
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - Han, Jinyu
AU  - Han J
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China.
FAU - Yuan, Zhengrong
AU  - Yuan Z
AUID- ORCID: 0000-0002-5175-0675
AD  - College of Biological Sciences and Biotechnology, Beijing Forestry University, 
      100083, Beijing, China. zryuan@bjfu.edu.cn.
FAU - Wang, Yajie
AU  - Wang Y
AD  - Department of Clinical Laboratory, Beijing Ditan Hospital, Capital Medical 
      University, 100015, Beijing, China. wangyajie@ccmu.edu.cn.
LA  - eng
GR  - Grant NO. 320.6750.19089-103/Wu Jieping Medical Foundation/
GR  - Grant NO. 320.6750.19089-75/Wu Jieping Medical Foundation/
PT  - Journal Article
DEP - 20220912
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Humans
MH  - *Carcinoma, Hepatocellular/metabolism
MH  - *Liver Neoplasms/metabolism
MH  - Prognosis
MH  - Gene Expression Profiling
MH  - Autophagy/genetics
MH  - Biomarkers, Tumor/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
OTO - NOTNLM
OT  - ATG3
OT  - ATG7
OT  - ATG9A
OT  - Hepatocellular carcinoma
OT  - Prognosis
OT  - TCGA
EDAT- 2022/09/13 06:00
MHDA- 2022/11/02 06:00
CRDT- 2022/09/12 23:59
PHST- 2021/12/10 00:00 [received]
PHST- 2022/03/24 00:00 [accepted]
PHST- 2022/03/23 00:00 [revised]
PHST- 2022/09/13 06:00 [pubmed]
PHST- 2022/11/02 06:00 [medline]
PHST- 2022/09/12 23:59 [entrez]
AID - 10.1007/s11033-022-07426-w [pii]
AID - 10.1007/s11033-022-07426-w [doi]
PST - ppublish
SO  - Mol Biol Rep. 2022 Nov;49(11):10269-10277. doi: 10.1007/s11033-022-07426-w. Epub 
      2022 Sep 12.