PMID- 36097830
OWN - NLM
STAT- MEDLINE
DCOM- 20240301
LR  - 20240301
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Linking)
VI  - 30
IP  - 2
DP  - 2024 Mar
TI  - Weakening of M1 macrophage and bone resorption in periodontitis cystathionine 
      gamma-lyase-deficient mice.
PG  - 769-779
LID - 10.1111/odi.14374 [doi]
AB  - OBJECTIVES: Cystathionine-gamma-lyase (CTH) has been proved to involve in 
      inflammation and bone remolding, implying its potential role in the progression 
      of periodontitis. This study was aimed to investigate the function of CTH and its 
      relation to the macrophage polarization in periodontitis. MATERIALS AND METHODS: 
      Bone marrow-derived macrophages (BMDMs) from wild-type (WT) and Cth knockout 
      (Cth(-/-) ) mice were stimulated with lipopolysaccharide (LPS) in vitro and 
      pro-inflammatory cytokines were analyzed by qRT-PCR. Ligature-induced 
      periodontitis was established on WT and Cth(-/-) mice. Histological analysis, 
      tartrate-resistant acid phosphatase staining, immunostaining, and Western blot 
      were performed to analyze the periodontium destruction and M1 macrophage 
      polarization. RESULTS: Cth expression in BMDMs was upregulated upon increasing 
      LPS stimulation. Deletion of Cth suppressed BMDMs inflammatory response with 
      decreased Il1b, Il6, and Tnf mRNA. Cth(-/-) mice with periodontitis showed 
      attenuated bone loss and impaired osteoclast differentiation compared with WT. 
      Moreover, Cth knockout hindered M1 macrophage polarization, reduced the 
      expression of IL-1beta, IL-6, and TNF-alpha in periodontally diseased tissue. 
      CONCLUSION: This study demonstrated that CTH played an important role in 
      regulating the inflammatory responses and periodontitis tissue destruction. 
      Importantly, Cth knockout suppressed M1 macrophages polarization in 
      periodontitis.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Li, Sijin
AU  - Li S
AD  - Department of Orthodontics, Stomatological Hospital and Dental School of Tongji 
      University, Shanghai Engineering Research Center of Tooth Restoration and 
      Regeneration, Shanghai, China.
FAU - Hua, Yongmei
AU  - Hua Y
AUID- ORCID: 0000-0001-7860-9375
AD  - Department of Orthodontics, Stomatological Hospital and Dental School of Tongji 
      University, Shanghai Engineering Research Center of Tooth Restoration and 
      Regeneration, Shanghai, China.
FAU - Liao, Chongshan
AU  - Liao C
AUID- ORCID: 0000-0001-7494-5515
AD  - Department of Orthodontics, Stomatological Hospital and Dental School of Tongji 
      University, Shanghai Engineering Research Center of Tooth Restoration and 
      Regeneration, Shanghai, China.
LA  - eng
GR  - 81870791/National Natural Science Foundation of China/
GR  - 82001008/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220927
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
RN  - 0 (Lipopolysaccharides)
MH  - Mice
MH  - Animals
MH  - Cystathionine gamma-Lyase/genetics/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/metabolism
MH  - *Bone Resorption
MH  - *Periodontitis/complications/metabolism
OTO - NOTNLM
OT  - M1 macrophage polarization
OT  - bone marrow-derived macrophages
OT  - cystathionine-gamma-lyase
OT  - inflammation
OT  - periodontitis
EDAT- 2022/09/14 06:00
MHDA- 2024/03/01 06:44
CRDT- 2022/09/13 04:07
PHST- 2022/08/23 00:00 [revised]
PHST- 2022/05/27 00:00 [received]
PHST- 2022/09/02 00:00 [accepted]
PHST- 2024/03/01 06:44 [medline]
PHST- 2022/09/14 06:00 [pubmed]
PHST- 2022/09/13 04:07 [entrez]
AID - 10.1111/odi.14374 [doi]
PST - ppublish
SO  - Oral Dis. 2024 Mar;30(2):769-779. doi: 10.1111/odi.14374. Epub 2022 Sep 27.