PMID- 36100121 OWN - NLM STAT- MEDLINE DCOM- 20221013 LR - 20221013 IS - 1873-6424 (Electronic) IS - 0269-7491 (Linking) VI - 313 DP - 2022 Nov 15 TI - BDE-209 and TCDD enhance metastatic characteristics of melanoma cells after chronic exposure. PG - 120140 LID - S0269-7491(22)01354-9 [pii] LID - 10.1016/j.envpol.2022.120140 [doi] AB - TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) and BDE-209 (decabromodiphenyl ether) are persistent organic pollutants (POPs) produced by industrial activities and associated with several diseases. TCDD is a known human carcinogen, but few studies investigated about the effects of exposure to both compounds, i.e., whether BDE-209 and TCDD can render tumor cells more aggressive and metastatic. In the current study we investigated if the exposure of B16-F1 and B16-F10 melanoma murine cells to environmental relevant concentrations of TCDD and BDE-209 at 24 h and 15-day exposure modulates the expression of genes related to metastasis, making the cells more aggressive. Both pollutants did not affect cell viability but lead to increase of cell proliferation, including the upregulation of vimentin, MMP2, MMP9, MMP14 and PGK1 gene expression and downregulation of E-cadherin, TIMP2, TIMP3 and RECK, strongly suggesting changes in cell phenotypes defined as epithelial to mesenchymal transition (EMT) in BDE-209 and TCDD-exposed cells. Foremost, increased expression of metalloproteinases and decreased expression of their inhibitors made B16-F1 cells similar the more aggressive B16-F10 cell line. Also, the higher secretion of extracellular vesicles by cells after acute exposure to BDE-209 could be related with the phenotype changes. These results are a strong indication of the potential of BDE-209 and TCDD to modulate cell phenotype, leading to a more aggressive profile. CI - Copyright (c) 2022 Elsevier Ltd. All rights reserved. FAU - Silva Filho, Benisio Ferreira AU - Silva Filho BF AD - Laboratorio de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil; Programa de Pos-Graduacao Em Biologia Celular e Molecular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil. FAU - Filipak Neto, Francisco AU - Filipak Neto F AD - Laboratorio de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil; Programa de Pos-Graduacao Em Biologia Celular e Molecular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil. FAU - Marchi, Micheli de AU - Marchi M AD - Laboratorio de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil; Programa de Pos-Graduacao Em Biologia Celular e Molecular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil. FAU - Moggio, Erick Laurent AU - Moggio EL AD - Laboratorio de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil; Programa de Pos-Graduacao Em Biologia Celular e Molecular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil. FAU - Rossi, Izadora Volpato AU - Rossi IV AD - Programa de Pos-Graduacao Em Biologia Celular e Molecular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil; Laboratorio de Biologia Molecular e Sistematica de Tripanossomatideos, Instituto Carlos Chagas -Fiocruz PR, CEP 81.350-010, Curitiba, PR, Brazil. FAU - Sabatke, Bruna AU - Sabatke B AD - Laboratorio de Biologia Molecular e Sistematica de Tripanossomatideos, Instituto Carlos Chagas -Fiocruz PR, CEP 81.350-010, Curitiba, PR, Brazil; Programa de Pos-Graduacao Em Microbiologia, Parasitologia e Patologia, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil. FAU - Ramirez, Marcel Ivan AU - Ramirez MI AD - Programa de Pos-Graduacao Em Biologia Celular e Molecular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil; Laboratorio de Biologia Molecular e Sistematica de Tripanossomatideos, Instituto Carlos Chagas -Fiocruz PR, CEP 81.350-010, Curitiba, PR, Brazil; Programa de Pos-Graduacao Em Microbiologia, Parasitologia e Patologia, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil. FAU - Lucena, Miguel Clodomiro Dos Santos AU - Lucena MCDS AD - Laboratorio de Glicobiologia Estrutural e Funcional. Instituto Carlos Chagas Filho. Universidade Federal Do Rio de Janeiro, CEP 21.941-902, Rio de Janeiro, RJ, Brazil. FAU - Todeschini, Adriane Regina AU - Todeschini AR AD - Laboratorio de Glicobiologia Estrutural e Funcional. Instituto Carlos Chagas Filho. Universidade Federal Do Rio de Janeiro, CEP 21.941-902, Rio de Janeiro, RJ, Brazil. FAU - Oliveira Ribeiro, Ciro Alberto de AU - Oliveira Ribeiro CA AD - Laboratorio de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil; Programa de Pos-Graduacao Em Biologia Celular e Molecular, Universidade Federal Do Parana, CEP 81.531-990, Curitiba, PR, Brazil. Electronic address: ciro@ufpr.br. LA - eng PT - Journal Article DEP - 20220910 PL - England TA - Environ Pollut JT - Environmental pollution (Barking, Essex : 1987) JID - 8804476 RN - 0 (Cadherins) RN - 0 (Carcinogens) RN - 0 (Environmental Pollutants) RN - 0 (GPI-Linked Proteins) RN - 0 (Halogenated Diphenyl Ethers) RN - 0 (Polychlorinated Dibenzodioxins) RN - 0 (RECK protein, human) RN - 0 (Vimentin) RN - EC 3.4.24.24 (Matrix Metalloproteinase 2) RN - EC 3.4.24.35 (Matrix Metalloproteinase 9) RN - EC 3.4.24.80 (Matrix Metalloproteinase 14) RN - N80BQ29A0H (decabromobiphenyl ether) SB - IM MH - Animals MH - Cadherins MH - Carcinogens MH - *Environmental Pollutants/pharmacology MH - Epithelial-Mesenchymal Transition MH - GPI-Linked Proteins MH - Halogenated Diphenyl Ethers MH - Humans MH - Matrix Metalloproteinase 14/pharmacology MH - Matrix Metalloproteinase 2/pharmacology MH - Matrix Metalloproteinase 9 MH - *Melanoma MH - Mice MH - Persistent Organic Pollutants MH - *Polychlorinated Dibenzodioxins/toxicity MH - Vimentin/pharmacology OTO - NOTNLM OT - Brominated compounds OT - Dioxin OT - Epithelial-mesenchymal transition OT - Metalloproteinases OT - Microvesicles COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2022/09/14 06:00 MHDA- 2022/10/14 06:00 CRDT- 2022/09/13 19:24 PHST- 2022/07/21 00:00 [received] PHST- 2022/09/01 00:00 [revised] PHST- 2022/09/05 00:00 [accepted] PHST- 2022/09/14 06:00 [pubmed] PHST- 2022/10/14 06:00 [medline] PHST- 2022/09/13 19:24 [entrez] AID - S0269-7491(22)01354-9 [pii] AID - 10.1016/j.envpol.2022.120140 [doi] PST - ppublish SO - Environ Pollut. 2022 Nov 15;313:120140. doi: 10.1016/j.envpol.2022.120140. Epub 2022 Sep 10.