PMID- 36100140
OWN - NLM
STAT- MEDLINE
DCOM- 20221115
LR  - 20221115
IS  - 1532-2939 (Electronic)
IS  - 0195-6701 (Print)
IS  - 0195-6701 (Linking)
VI  - 130
DP  - 2022 Dec
TI  - Efficiency and sensitivity optimization of a protocol to quantify indoor airborne 
      SARS-CoV-2 levels.
PG  - 44-51
LID - S0195-6701(22)00274-2 [pii]
LID - 10.1016/j.jhin.2022.08.011 [doi]
AB  - BACKGROUND: Development of methodologies to quantify airborne micro-organisms is 
      needed for the prevention and control of infections. It is difficult to conclude 
      which is the most efficient and sensitive strategy to assess airborne SARS-CoV-2 
      RNA levels due to the disparity of results reported in clinical settings. AIM: To 
      improve our previously reported protocol of measuring SARS-CoV-2 RNA levels, 
      which was based on bioaerosol collection with a liquid impinger and RNA 
      quantification with droplet digital polymerase chain reaction (ddPCR). METHODS: 
      Air samples were collected in COVID-19 patient rooms to assess efficiency and/or 
      sensitivity of different air samplers, liquid collection media, and reverse 
      transcriptases (RT). FINDINGS: Mineral oil retains airborne RNA better than does 
      hydrophilic media without impairing integrity. SARS-CoV-2 ORF1ab target was 
      detected in 80% of the air samples using BioSampler with mineral oil. No 
      significant differences in effectiveness were obtained with MD8 sampler equipped 
      with gelatine membrane filters, but the SARS-CoV-2 copies/m(3) air obtained with 
      the latter were lower (28.4 +/- 6.1 vs 9 +/- 1.7). SuperScript II RT allows the 
      detection of a single SARS-CoV-2 genome RNA molecule by ddPCR with high 
      efficiency. This was the only RT that allowed the detection of SARS-CoV-2 N1 
      target in air samples. CONCLUSION: The collection efficiency and detection 
      sensivity of a protocol to quantify SARS-CoV-2 RNA levels in indoor air has been 
      improved in the present study. Such optimization is important to improve our 
      understanding of the microbiological safety of indoor air.
CI  - Copyright (c) 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Truyols-Vives, J
AU  - Truyols-Vives J
AD  - Molecular Biology and One Health Research Group (MolONE), Universitat de les 
      Illes Balears (UIB), Palma, Spain.
FAU - Stiliyanov-Atanasov, K
AU  - Stiliyanov-Atanasov K
AD  - Molecular Biology and One Health Research Group (MolONE), Universitat de les 
      Illes Balears (UIB), Palma, Spain.
FAU - Sala-Llinas, E
AU  - Sala-Llinas E
AD  - Molecular Biology and One Health Research Group (MolONE), Universitat de les 
      Illes Balears (UIB), Palma, Spain; Health Research Institute of the Balearic 
      Islands (IdISBa), Balearic Islands, Spain; Department of Pulmonary Medicine, 
      Hospital Universitari Son Espases (HUSE), Balearic Islands, Spain; Biomedical 
      Research Networking Center on Respiratory Diseases (CIBERES), Madrid, Spain.
FAU - Toledo-Pons, N
AU  - Toledo-Pons N
AD  - Health Research Institute of the Balearic Islands (IdISBa), Balearic Islands, 
      Spain; Department of Pulmonary Medicine, Hospital Universitari Son Espases 
      (HUSE), Balearic Islands, Spain.
FAU - Baldovi, H G
AU  - Baldovi HG
AD  - Department of Chemistry, Universitat Politecnica de Valencia, Valencia, Spain.
FAU - Mercader-Barcelo, J
AU  - Mercader-Barcelo J
AD  - Molecular Biology and One Health Research Group (MolONE), Universitat de les 
      Illes Balears (UIB), Palma, Spain; Health Research Institute of the Balearic 
      Islands (IdISBa), Balearic Islands, Spain. Electronic address: 
      Josep.mercader@uib.es.
LA  - eng
PT  - Journal Article
DEP - 20220912
PL  - England
TA  - J Hosp Infect
JT  - The Journal of hospital infection
JID - 8007166
RN  - 0 (RNA, Viral)
RN  - 8020-83-5 (Mineral Oil)
SB  - IM
MH  - Humans
MH  - *SARS-CoV-2/genetics
MH  - *COVID-19/prevention & control
MH  - RNA, Viral/genetics
MH  - Mineral Oil
PMC - PMC9465472
OTO - NOTNLM
OT  - Bioaerosol
OT  - Droplet digital PCR
OT  - Impinger
OT  - SARS-CoV-2
EDAT- 2022/09/14 06:00
MHDA- 2022/11/16 06:00
PMCR- 2022/09/12
CRDT- 2022/09/13 19:25
PHST- 2022/07/29 00:00 [received]
PHST- 2022/08/24 00:00 [revised]
PHST- 2022/08/26 00:00 [accepted]
PHST- 2022/09/14 06:00 [pubmed]
PHST- 2022/11/16 06:00 [medline]
PHST- 2022/09/13 19:25 [entrez]
PHST- 2022/09/12 00:00 [pmc-release]
AID - S0195-6701(22)00274-2 [pii]
AID - 10.1016/j.jhin.2022.08.011 [doi]
PST - ppublish
SO  - J Hosp Infect. 2022 Dec;130:44-51. doi: 10.1016/j.jhin.2022.08.011. Epub 2022 Sep 
      12.