PMID- 3610158
OWN - NLM
STAT- MEDLINE
DCOM- 19870916
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 76
IP  - 4
DP  - 1987 Aug
TI  - Confirmation of assignment of the human alpha 1-crystallin gene (CRYA1) to 
      chromosome 21 with regional localization to q22.3.
PG  - 375-80
AB  - The crystallins are highly conserved structural proteins universally found in the 
      eye lens of all vertebrate species. In mammals, three immunologically distinct 
      classes are present, alpha-, beta-, and gamma-crystallins, and each class 
      represents a multigene family. The alpha-crystallin gene family consists of alpha 
      1-crystallin (CRYA1) and alpha 2-crystallin (CRYA2) genes (previously designated 
      alpha A- and alpha B-crystallin, respectively), which show extensive sequence 
      homology. We constructed a synthetic oligonucleotide probe of 25 bases 
      corresponding to a specific region of the human alpha 1-crystallin gene sequence. 
      This 25-mer probe bears little sequence homology to human alpha 2-crystallin gene 
      and does not cross-hybridize to alpha 2-crystallin sequences in Southern blot 
      analysis. Using this unique synthetic probe, we have demonstrated the identity of 
      the alpha 1-crystallin gene in human genomic DNA. In addition, we have also 
      confirmed its chromosomal location on human chromosome 21. Finally, we have 
      regionally localized the gene to q22.3 by using both Southern blot analysis of a 
      panel of cell hybrids containing different parts of human chromosome 21, and in 
      situ hybridization to metaphase chromosomes. The use of synthetic oligonucleotide 
      probes specific for individual genes should be useful in identifying and mapping 
      members of multigene families.
FAU - Hawkins, J W
AU  - Hawkins JW
FAU - Van Keuren, M L
AU  - Van Keuren ML
FAU - Piatigorsky, J
AU  - Piatigorsky J
FAU - Law, M L
AU  - Law ML
FAU - Patterson, D
AU  - Patterson D
FAU - Kao, F T
AU  - Kao FT
LA  - eng
GR  - HD02080/HD/NICHD NIH HHS/United States
GR  - HD17449/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (Crystallins)
SB  - IM
MH  - Animals
MH  - Chromosome Banding
MH  - *Chromosome Mapping
MH  - *Chromosomes, Human, Pair 21
MH  - Cricetinae
MH  - Cricetulus
MH  - Crystallins/*genetics
MH  - Humans
MH  - Hybrid Cells
MH  - Karyotyping
MH  - Nucleic Acid Hybridization
EDAT- 1987/08/01 00:00
MHDA- 1987/08/01 00:01
CRDT- 1987/08/01 00:00
PHST- 1987/08/01 00:00 [pubmed]
PHST- 1987/08/01 00:01 [medline]
PHST- 1987/08/01 00:00 [entrez]
AID - 10.1007/BF00272448 [doi]
PST - ppublish
SO  - Hum Genet. 1987 Aug;76(4):375-80. doi: 10.1007/BF00272448.