PMID- 36103172 OWN - NLM STAT- MEDLINE DCOM- 20221115 LR - 20221115 IS - 1600-0765 (Electronic) IS - 0022-3484 (Linking) VI - 57 IP - 6 DP - 2022 Dec TI - Expression profile analysis of long noncoding RNA and messenger RNA during mouse cementoblast mineralization. PG - 1159-1168 LID - 10.1111/jre.13053 [doi] AB - BACKGROUND AND OBJECTIVE: Emerging evidence has uncovered that long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) exert biofunctions on cellular mineralization and bone formation. In this study, we aimed to identify lncRNA-mRNA expression profiles and expression patterns, and explore their underlying biofunctions during cementoblast mineralization. MATERIALS AND METHODS: Cementoblasts were cultured in mineralized medium for 0, 7, and 14 days. We used quantitative real-time polymerase chain reaction (qRT-PCR) and western blot (WB) to detect expression levels of osteocalcin (OCN), bone sialoprotein (BSP), and Osterix (Osx). Alkaline phosphatase (ALP) staining and alizarin red staining (ARS) were conducted to detect ALP activity and number of mineralized nodule. Total RNA was extracted from cells and used for high-throughput sequencing. EBSeq package was applied to analyze differentially expressed genes. Mfuzz R package was used to identify gene expression patterns. The weighted gene co-expression network analysis (WGCNA) was performed to explore co-expressed mRNAs of differentially expressed lncRNAs (DElncRNAs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were adopted by Clusterprofile R package. RESULTS: Cementoblasts were successfully induced by osteogenic medium. Compared with those on day 0, 384 DElncRNAs and 4255 differentially expressed mRNAs (DEmRNAs), respectively, were found on day 7. Meanwhile, 645 DElncRNAs and 4717 DEmRNAs were detected on day 14. Both DElncRNAs and DEmRNAs were classified into six clusters with different expression patterns. DEmRNAs and co-expressed mRNA of DElncRNAs were predominantly related to cell process, binding, phosphatidylinositol-3 kinase (PI3K)-Akt signaling pathway, hypoxia-inducible factor-1 (HIF-1) signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, and hippo signaling pathway. CONCLUSION: The results demonstrated that both noncoding and coding RNAs were involved in the process of mineralization in cementoblasts, which may provide a new database for further study. CI - (c) 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Huang, Hantao AU - Huang H AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Wang, Xiaoxuan AU - Wang X AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. AD - Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Liao, Haiqing AU - Liao H AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. AD - Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, China. FAU - Ma, Li AU - Ma L AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. AD - Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Jiang, Chenxi AU - Jiang C AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Yao, Siqi AU - Yao S AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Liu, Huan AU - Liu H AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. AD - Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan, China. FAU - Cao, Zhengguo AU - Cao Z AUID- ORCID: 0000-0002-4261-9034 AD - The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST KLOS) and Key Laboratory for Oral Biomedical Engineering of Ministry of Education (KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. AD - Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan, China. LA - eng GR - National Natural Science Foundation of China/ PT - Journal Article DEP - 20220914 PL - United States TA - J Periodontal Res JT - Journal of periodontal research JID - 0055107 RN - 0 (RNA, Long Noncoding) RN - 0 (RNA, Messenger) SB - IM MH - Mice MH - Animals MH - *RNA, Long Noncoding/genetics MH - RNA, Messenger/genetics MH - Dental Cementum MH - Gene Ontology MH - High-Throughput Nucleotide Sequencing OTO - NOTNLM OT - cementoblast OT - lncRNA OT - mRNA OT - mineralization EDAT- 2022/09/15 06:00 MHDA- 2022/11/16 06:00 CRDT- 2022/09/14 11:33 PHST- 2022/08/17 00:00 [revised] PHST- 2022/05/15 00:00 [received] PHST- 2022/08/29 00:00 [accepted] PHST- 2022/09/15 06:00 [pubmed] PHST- 2022/11/16 06:00 [medline] PHST- 2022/09/14 11:33 [entrez] AID - 10.1111/jre.13053 [doi] PST - ppublish SO - J Periodontal Res. 2022 Dec;57(6):1159-1168. doi: 10.1111/jre.13053. Epub 2022 Sep 14.