PMID- 36104516
OWN - NLM
STAT- MEDLINE
DCOM- 20230301
LR  - 20230301
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Print)
IS  - 0360-3997 (Linking)
VI  - 46
IP  - 1
DP  - 2023 Feb
TI  - Transmembrane TNF-alpha as a Novel Biomarker for the Diagnosis of Cytokine Storms in 
      a Mouse Model of Multiple Organ Failure.
PG  - 359-369
LID - 10.1007/s10753-022-01738-6 [doi]
AB  - A cytokine storm (CS) is an out-of-control inflammatory response closely 
      associated with the progression of diseases, such as multiple organ failure 
      (MOF), severe sepsis, and severe or critical COVID-19. However, there is 
      currently a lack of reliable diagnostic markers to distinguish CS from normal 
      inflammatory responses. Tumor necrosis factor-alpha (TNF-alpha) includes transmembrane 
      TNF-alpha (tmTNF-alpha) and secreted TNF-alpha (sTNF-alpha). The MOF mouse model in this study 
      showed that the tmTNF-alpha expression changes in the neutrophils differed from the 
      serum TNF-alpha and serum IL-18, INF-gamma, IL-4, and IL-6. Furthermore, tmTNF-alpha, instead 
      of serum TNF-alpha, IL-18, INF-gamma, IL-4, and IL-6, reflected liver and kidney tissue 
      damage and increased with the aggravation of these injuries. Analysis of the ROC 
      results showed that tmTNF-alpha effectively distinguished between inflammatory 
      responses and CS and efficiently differentiated between surviving and dead mice. 
      It also significantly improved the diagnostic value of the traditional CRP marker 
      for CS. These results indicated that the tmTNF-alpha expressed in the neutrophils 
      could be used to diagnose CS in MOF mice, providing an experimental basis to 
      further develop tmTNF-alpha for diagnosing CS patients.
CI  - (c) 2022. The Author(s).
FAU - Yang, Peng
AU  - Yang P
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital, Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550001, China.
FAU - Zeng, Yimin
AU  - Zeng Y
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital, Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550001, China.
FAU - Yang, Fang
AU  - Yang F
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital, Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550001, China.
FAU - Peng, Xin
AU  - Peng X
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital, Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550001, China.
FAU - Hu, Yongsheng
AU  - Hu Y
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital, Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550001, China.
FAU - Tan, Xuhong
AU  - Tan X
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital, Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550001, China.
FAU - Zhang, Ruping
AU  - Zhang R
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital, Guizhou 
      University of Traditional Chinese Medicine, Guiyang, 550001, China. 
      468050863@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20220914
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Interleukin-18)
RN  - 207137-56-2 (Interleukin-4)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Tumor Necrosis Factor-alpha/metabolism
MH  - Interleukin-6/metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I/metabolism
MH  - Interleukin-18
MH  - Multiple Organ Failure/diagnosis
MH  - Interleukin-4
MH  - Cytokine Release Syndrome
MH  - *COVID-19
MH  - Biomarkers
MH  - COVID-19 Testing
PMC - PMC9473472
OTO - NOTNLM
OT  - Cytokine storm
OT  - Diagnostic marker
OT  - Inflammatory responses
OT  - Multiple organ failure
OT  - Transmembrane TNF-alpha
COIS- The authors declare no competing interests.
EDAT- 2022/09/15 06:00
MHDA- 2023/03/03 06:00
PMCR- 2022/09/14
CRDT- 2022/09/14 23:31
PHST- 2022/06/15 00:00 [received]
PHST- 2022/09/08 00:00 [accepted]
PHST- 2022/09/08 00:00 [revised]
PHST- 2022/09/15 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2022/09/14 23:31 [entrez]
PHST- 2022/09/14 00:00 [pmc-release]
AID - 10.1007/s10753-022-01738-6 [pii]
AID - 1738 [pii]
AID - 10.1007/s10753-022-01738-6 [doi]
PST - ppublish
SO  - Inflammation. 2023 Feb;46(1):359-369. doi: 10.1007/s10753-022-01738-6. Epub 2022 
      Sep 14.