PMID- 36105024
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220917
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 14
IP  - 8
DP  - 2022
TI  - Candidate metabolite markers of peripheral neuropathy in Chinese patients with 
      type 2 diabetes.
PG  - 5420-5440
AB  - OBJECTIVES: To analyze the serum and urine metabolites present in type 2 diabetes 
      mellitus (T2DM) patients and T2DM patients with diabetic peripheral neuropathy 
      (DPN) and to select differentially expressed biomarkers for early diagnosis of 
      DPN. METHODS: Serum and urine metabolites from 74 T2DM patients with peripheral 
      neuropathy and 41 without peripheral neuropathy were analyzed using gas 
      chromatograph system with time-of-flight mass spectrometer metabolomics to detect 
      biomarkers of peripheral neuropathy in T2DM. RESULTS: There were increased serum 
      triglycerides, alanine aminotransferase, and decreased C-peptide, and total 
      cholesterol levels in T2DM patients with DPN compared to those without peripheral 
      neuropathy. Metabolomic analysis revealed visible differences in metabolic 
      characteristics between two groups, and overall 53 serum differential metabolites 
      and 56 urine differential metabolites were identified with variable influence on 
      projection (VIP) >1 and P<0.05. To further analyze the correlation between the 
      identified metabolites and DPN, four serum metabolites and six urine metabolites 
      were selected with VIP>2, and fold change (FC) >1, including serum beta-alanine, 
      caproic acid, beta-alanine/L-aspartic acid, and L-arabinose/L-arabitol, and urine 
      gluconic acid, erythritol, galactonic acid, guanidoacetic acid, cytidine, and 
      aminoadipic acid. Furthermore, five serum biomarkers and six urine biomarkers 
      were found to show significant changes (P<0.05, VIP>1, and FC>1) respectively in 
      patients with mild, moderate, and severe DPN. In addition, we found that 
      glyoxylate and dicarboxylate metabolism was a differential metabolic pathway not 
      only between T2DM and DPN, but also among different degrees of DPN. The 
      differential metabolites such as beta-alanine and caproic acid are expected to be 
      biomarkers for DPN patients, and the significant changes in glyoxylate and 
      dicarboxylate metabolism may be related to the pathogenesis of DPN. CONCLUSION: 
      There were serum and urine spectrum metabolomic differences in patients with DPN, 
      which could serve as biomarkers for T2DM and DPN patients.
CI  - AJTR Copyright (c) 2022.
FAU - Shao, Ming-Mei
AU  - Shao MM
AD  - Institute of Interdisciplinary Integrative Medicine Research, Shanghai University 
      of Traditional Chinese Medicine Shanghai 201203, China.
AD  - Institute of Digestive Disease, Longhua Hospital, Shanghai University of 
      Traditional Chinese Medicine Shanghai 200032, China.
FAU - Xiang, Hong-Jiao
AU  - Xiang HJ
AD  - Institute of Interdisciplinary Integrative Medicine Research, Shanghai University 
      of Traditional Chinese Medicine Shanghai 201203, China.
AD  - Institute of Digestive Disease, Longhua Hospital, Shanghai University of 
      Traditional Chinese Medicine Shanghai 200032, China.
FAU - Lu, Hao
AU  - Lu H
AD  - Department of Endocrinology and Metabolism, Shuguang Hospital Affiliated to 
      Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
FAU - Yin, Pei-Hao
AU  - Yin PH
AD  - Putuo Hospital, Shanghai University of Traditional Chinese Medicine Shanghai 
      200062, China.
FAU - Li, Guo-Wen
AU  - Li GW
AD  - Pharmacy Department, Shanghai TCM-Integrated Hospital, Shanghai University of 
      Traditional Chinese Medicine Shanghai 200071, China.
FAU - Wang, Yun-Man
AU  - Wang YM
AD  - Putuo Hospital, Shanghai University of Traditional Chinese Medicine Shanghai 
      200062, China.
FAU - Chen, Lin
AU  - Chen L
AD  - Putuo Hospital, Shanghai University of Traditional Chinese Medicine Shanghai 
      200062, China.
FAU - Chen, Qing-Guang
AU  - Chen QG
AD  - Department of Endocrinology and Metabolism, Shuguang Hospital Affiliated to 
      Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
FAU - Zhao, Cheng
AU  - Zhao C
AD  - Pharmacy Department, Shanghai TCM-Integrated Hospital, Shanghai University of 
      Traditional Chinese Medicine Shanghai 200071, China.
FAU - Lu, Qun
AU  - Lu Q
AD  - Pharmacy Department, Shanghai TCM-Integrated Hospital, Shanghai University of 
      Traditional Chinese Medicine Shanghai 200071, China.
FAU - Wu, Tao
AU  - Wu T
AD  - Institute of Interdisciplinary Integrative Medicine Research, Shanghai University 
      of Traditional Chinese Medicine Shanghai 201203, China.
AD  - Institute of Digestive Disease, Longhua Hospital, Shanghai University of 
      Traditional Chinese Medicine Shanghai 200032, China.
FAU - Ji, Guang
AU  - Ji G
AD  - Institute of Digestive Disease, Longhua Hospital, Shanghai University of 
      Traditional Chinese Medicine Shanghai 200032, China.
LA  - eng
PT  - Journal Article
DEP - 20220815
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC9452362
OTO - NOTNLM
OT  - Type 2 diabetes mellitus
OT  - diabetic peripheral neuropathy
OT  - gas chromatograph system with time-of-flight mass spectrometer
OT  - metabolomics
COIS- None.
EDAT- 2022/09/16 06:00
MHDA- 2022/09/16 06:01
PMCR- 2022/08/15
CRDT- 2022/09/15 02:14
PHST- 2022/04/23 00:00 [received]
PHST- 2022/07/17 00:00 [accepted]
PHST- 2022/09/15 02:14 [entrez]
PHST- 2022/09/16 06:00 [pubmed]
PHST- 2022/09/16 06:01 [medline]
PHST- 2022/08/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2022 Aug 15;14(8):5420-5440. eCollection 2022.