PMID- 36105029
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220917
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 14
IP  - 8
DP  - 2022
TI  - Acupuncture relieves the visceral pain of diarrhea-predominant irritable bowel 
      syndrome rats by regulating P2X4 expression.
PG  - 5563-5573
AB  - OBJECTIVES: We researched the effect and mechanism of acupuncture treatment for 
      visceral pain in rats with diarrhea-predominant irritable bowel syndrome (IBS-D). 
      METHODS: We set up a rat model of IBS-D with chemical and chronic- and 
      acute-pressure stimulations. Then, the IBS-D rats were treated with acupuncture 
      or 5-BDBD, and the therapeutic efficacy of acupuncture in IBS-D rats was assessed 
      by means of the Bristol scale, diarrhea index, abdominal withdrawal reflex (AWR) 
      score, mast cell count and histologic staining. RESULTS: Acupuncture 
      significantly decreased clinical symptoms in IBS-D rats after a 14 day-treatment. 
      Furthermore, significant down-regulation of P2X4, OX42, BDNF (brain-derived 
      neurotrophic factor) and IRF-5 (interferon regulatory factor 5) expressions were 
      observed in the IBS-D rats, along with the decreased inflammatory factors 
      [interleukin 1 beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), and interleukin 
      6 (IL-6)], chemokines [monocyte chemoattractant protein-1 (MCP-1), regulated on 
      activation, normal T cell expressed and secreted (RANTES), and C-X-C motif 
      chemokine ligand 1 (CXCL1)], and neurotransmitters [substance P (SP), 
      5-hydroxytryptamine (5-HT), and calcitonin gene-related peptide (CGRP)]. 5-BDBD 
      treatment had a similar effect on IBS-D rats. CONCLUSIONS: Acupuncture can 
      effectively alleviate abdominal pain by decreasing visceral hypersensitivity and 
      controlling the expression of P2X4 and spinal microglial inflammation in IBS 
      rats.
CI  - AJTR Copyright (c) 2022.
FAU - Tang, He-Yong
AU  - Tang HY
AD  - School of Integrated Traditional Chinese and Western Medicine, Anhui University 
      of Traditional Chinese Medicine Hefei 230012, Anhui, China.
FAU - Chen, Xi-Qiuyu
AU  - Chen XQ
AD  - School of Integrated Traditional Chinese and Western Medicine, Anhui University 
      of Traditional Chinese Medicine Hefei 230012, Anhui, China.
FAU - Wang, Hao
AU  - Wang H
AD  - School of Integrated Traditional Chinese and Western Medicine, Anhui University 
      of Traditional Chinese Medicine Hefei 230012, Anhui, China.
FAU - Chu, Hao-Ran
AU  - Chu HR
AD  - The Second Affiliated Hospital of Anhui University of Traditional Chinese 
      Medicine Hefei 230012, Anhui, China.
FAU - Zhu, Cai-Feng
AU  - Zhu CF
AD  - The Second Affiliated Hospital of Anhui University of Traditional Chinese 
      Medicine Hefei 230012, Anhui, China.
FAU - Huang, Shun
AU  - Huang S
AD  - School of Integrated Traditional Chinese and Western Medicine, Anhui University 
      of Traditional Chinese Medicine Hefei 230012, Anhui, China.
FAU - Zhang, Meng-Ting
AU  - Zhang MT
AD  - School of Integrated Traditional Chinese and Western Medicine, Anhui University 
      of Traditional Chinese Medicine Hefei 230012, Anhui, China.
FAU - Shen, Guo-Ming
AU  - Shen GM
AD  - School of Integrated Traditional Chinese and Western Medicine, Anhui University 
      of Traditional Chinese Medicine Hefei 230012, Anhui, China.
LA  - eng
PT  - Journal Article
DEP - 20220815
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC9452363
OTO - NOTNLM
OT  - IBS-D rat
OT  - P2X4
OT  - acupuncture
OT  - visceral pain
COIS- None.
EDAT- 2022/09/16 06:00
MHDA- 2022/09/16 06:01
PMCR- 2022/08/15
CRDT- 2022/09/15 02:14
PHST- 2022/05/26 00:00 [received]
PHST- 2022/07/14 00:00 [accepted]
PHST- 2022/09/15 02:14 [entrez]
PHST- 2022/09/16 06:00 [pubmed]
PHST- 2022/09/16 06:01 [medline]
PHST- 2022/08/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2022 Aug 15;14(8):5563-5573. eCollection 2022.