PMID- 36106658
OWN - NLM
STAT- MEDLINE
DCOM- 20221122
LR  - 20221122
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 85
IP  - 12
DP  - 2022 Dec
TI  - Microanatomy of the cerebellum in Persian squirrel (Sciurus anomalus), a 
      transmission electron microscopy, and immunohistochemical study.
PG  - 3850-3859
LID - 10.1002/jemt.24234 [doi]
AB  - The present study was aimed to investigate the cerebellum histology in the 
      Persian squirrel (Sciurus anomalus), using immunohistochemical staining and 
      transmission electron microscopy (TEM). The brain of 4 adult Persian squirrels (2 
      males and 2 females) were used. For general histology, crystal violet and Luxol 
      fast blue stains were applied. For immunohistochemical examination, myelin 
      associated glycoprotein (MAG), neurofilament 200 (NF200), Calbindin D 28 K, and 
      glial fibrillary associated protein (GFAP) were investigated. The cerebellar 
      cortex was composed of three traditional cellular layers, including molecular, 
      granular and Purkinje cell (Pc) layers. Numerous Nissl bodies detected in the 
      cytoplasm of Pc, which given a purple or blue appearance to the cytoplasm. At the 
      level of TEM, Purkinje cells were identified by their large euchromatin nucleus 
      with an indented plasma membrane. The cytoplasm was high electron dense 
      containing well-developed rough endoplasmic reticulum (Er), smooth Er, Golgi 
      apparatus in perinuclear region, numerous mitochondria in oval and spherical 
      shapes, free ribosomes, and lysosomes were dispersed throughout the cytoplasm. 
      The expression rate of calbindin D 28 K, MAG, GFAP, and NF200 was determined 49%, 
      24%, 71%, and 43% respectively. The cerebellum of the Persian squirrel has a 
      common structure similar to those reported in other mammals. However, at the 
      level of TEM, some unique structural features were observed. These results might 
      be taken as a set of basic information for more detailed future studies 
      investigating histophysiology of the cerebellum and its pathological changes in 
      the Persian squirrel as well as for comparative purposes in rodent species.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Nowrozi, Masoumeh
AU  - Nowrozi M
AD  - D.V.M student, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
FAU - Goodarzi, Nader
AU  - Goodarzi N
AUID- ORCID: 0000-0003-4704-6076
AD  - Department of Basic Sciences and Pathobiology, Faculty of Veterinary Medicine, 
      Razi University, Kermanshah, Iran.
LA  - eng
GR  - Deputy of Research and Technology, Razi University/
PT  - Journal Article
DEP - 20220915
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Calbindins)
SB  - IM
MH  - Animals
MH  - Male
MH  - Female
MH  - *Sciuridae
MH  - *Cerebellum
MH  - Purkinje Cells
MH  - Microscopy, Electron, Transmission
MH  - Calbindins
OTO - NOTNLM
OT  - Purkinje cell
OT  - calbindin
OT  - central nervous system
OT  - rodent
EDAT- 2022/09/16 06:00
MHDA- 2022/11/23 06:00
CRDT- 2022/09/15 06:26
PHST- 2022/08/16 00:00 [revised]
PHST- 2022/06/06 00:00 [received]
PHST- 2022/09/01 00:00 [accepted]
PHST- 2022/09/16 06:00 [pubmed]
PHST- 2022/11/23 06:00 [medline]
PHST- 2022/09/15 06:26 [entrez]
AID - 10.1002/jemt.24234 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2022 Dec;85(12):3850-3859. doi: 10.1002/jemt.24234. Epub 2022 
      Sep 15.