PMID- 36108089
OWN - NLM
STAT- MEDLINE
DCOM- 20220929
LR  - 20221007
IS  - 1553-7374 (Electronic)
IS  - 1553-7366 (Print)
IS  - 1553-7366 (Linking)
VI  - 18
IP  - 9
DP  - 2022 Sep
TI  - A tRNA-derived fragment present in E. coli OMVs regulates host cell gene 
      expression and proliferation.
PG  - e1010827
LID - 10.1371/journal.ppat.1010827 [doi]
LID - e1010827
AB  - RNA-sequencing has led to a spectacular increase in the repertoire of bacterial 
      sRNAs and improved our understanding of their biological functions. Bacterial 
      sRNAs have also been found in outer membrane vesicles (OMVs), raising questions 
      about their potential involvement in bacteria-host relationship, but few studies 
      have documented this issue. Recent RNA-Sequencing analyses of bacterial RNA 
      unveiled the existence of abundant very small RNAs (vsRNAs) shorter than 16 nt. 
      These especially include tRNA fragments (tRFs) that are selectively loaded in 
      OMVs and are predicted to target host mRNAs. Here, in Escherichia coli (E. coli), 
      we report the existence of an abundant vsRNA, Ile-tRF-5X, which is selectively 
      modulated by environmental stress, while remaining unaffected by inhibition of 
      transcription or translation. Ile-tRF-5X is released through OMVs and can be 
      transferred to human HCT116 cells, where it promoted MAP3K4 expression. Our 
      findings provide a novel perspective and paradigm on the existing symbiosis 
      between bacteria and human cells.
FAU - Diallo, Idrissa
AU  - Diallo I
AD  - CHU de Quebec-Universite Laval Research Center/CHUL Pavilion, Department of 
      Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universite 
      Laval, Quebec City, Quebec, Canada.
FAU - Ho, Jeffrey
AU  - Ho J
AD  - CHU de Quebec-Universite Laval Research Center/CHUL Pavilion, Department of 
      Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universite 
      Laval, Quebec City, Quebec, Canada.
FAU - Lambert, Marine
AU  - Lambert M
AD  - CHU de Quebec-Universite Laval Research Center/CHUL Pavilion, Department of 
      Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universite 
      Laval, Quebec City, Quebec, Canada.
FAU - Benmoussa, Abderrahim
AU  - Benmoussa A
AD  - CHU de Quebec-Universite Laval Research Center/CHUL Pavilion, Department of 
      Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universite 
      Laval, Quebec City, Quebec, Canada.
FAU - Husseini, Zeinab
AU  - Husseini Z
AD  - CHU de Quebec-Universite Laval Research Center/CHUL Pavilion, Department of 
      Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universite 
      Laval, Quebec City, Quebec, Canada.
FAU - Lalaouna, David
AU  - Lalaouna D
AD  - CRCHUS, RNA Group, Department of Biochemistry and Functional Genomics, Faculty of 
      Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Quebec, 
      Canada.
FAU - Masse, Eric
AU  - Masse E
AD  - CRCHUS, RNA Group, Department of Biochemistry and Functional Genomics, Faculty of 
      Medicine and Health Sciences, Universite de Sherbrooke, Sherbrooke, Quebec, 
      Canada.
FAU - Provost, Patrick
AU  - Provost P
AUID- ORCID: 0000-0002-6099-6562
AD  - CHU de Quebec-Universite Laval Research Center/CHUL Pavilion, Department of 
      Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universite 
      Laval, Quebec City, Quebec, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220915
PL  - United States
TA  - PLoS Pathog
JT  - PLoS pathogens
JID - 101238921
RN  - 0 (RNA, Bacterial)
RN  - 9014-25-9 (RNA, Transfer)
SB  - IM
MH  - Cell Proliferation
MH  - *Escherichia coli/genetics
MH  - Gene Expression
MH  - Humans
MH  - *RNA, Bacterial/genetics
MH  - RNA, Transfer/genetics
PMC - PMC9514646
COIS- The authors have declared that no competing interests exist.
EDAT- 2022/09/16 06:00
MHDA- 2022/09/30 06:00
PMCR- 2022/09/15
CRDT- 2022/09/15 14:06
PHST- 2022/04/09 00:00 [received]
PHST- 2022/08/22 00:00 [accepted]
PHST- 2022/09/27 00:00 [revised]
PHST- 2022/09/16 06:00 [pubmed]
PHST- 2022/09/30 06:00 [medline]
PHST- 2022/09/15 14:06 [entrez]
PHST- 2022/09/15 00:00 [pmc-release]
AID - PPATHOGENS-D-22-00647 [pii]
AID - 10.1371/journal.ppat.1010827 [doi]
PST - epublish
SO  - PLoS Pathog. 2022 Sep 15;18(9):e1010827. doi: 10.1371/journal.ppat.1010827. 
      eCollection 2022 Sep.