PMID- 36119614
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220920
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 13
DP  - 2022
TI  - Multi-omics analysis of the regulatory effects of low-phosphorus stress on 
      phosphorus transport in soybean roots.
PG  - 992036
LID - 10.3389/fpls.2022.992036 [doi]
LID - 992036
AB  - The regulatory effects of uneven phosphorus supplies on phosphorus transport in 
      soybean roots are still unclear. To further analyze the regulatory effects of 
      low-phosphorus stress on phosphorus transport in soybean roots and the effects of 
      uneven phosphorus application on the physiological mechanism of phosphorus 
      transport in soybean roots, dual-root soybean plants were prepared via grafting, 
      and a sand culture experiment was performed. From the unfolded cotyledon stage to 
      the initial flowering stage, one side of each dual-root soybean system was 
      irrigated with a low-phosphorus-concentration solution (phosphorus-application 
      [P+] side), and the other side was irrigated with a phosphorus-free nutrient 
      solution (phosphorus-free [P-] side); this setup allowed the study of the effects 
      of different phosphorus supply levels on the expression of genes and proteins and 
      the accumulation of metabolites in soybean roots on the P- side to clarify the 
      method through which phosphorus transport is regulated in soybean roots and to 
      provide a theoretical basis for improving the use rate of phosphorus fertilizer. 
      The results revealed that the unilateral supply of low-concentration phosphorus 
      promoted the uptake of phosphorus by soybean roots and the transport of 
      phosphorus from the P+ side to the P- side. Compared with the normal 
      concentration of phosphorus supply and the phosphorus-free supply, the low 
      concentration phosphorus supply affected the regulation of the metabolic pathways 
      involved in starch and sucrose metabolism, glycolysis, fructose, and mannose 
      metabolism, etc., thereby affecting soybean root phosphorus transport. The 
      low-phosphorus stress inhibited fructose synthesis and sucrose synthase synthesis 
      in the soybean roots and the synthesis of hexokinase (HK) and fructose kinase, 
      which catalyzes the conversion of fructose to fructose-6-phosphate. 
      Low-phosphorus stress promoted the synthesis of sucrose invertase and the 
      conversion of sucrose into maltose by the activity of starch synthase (StS) and 
      stimulated the synthesis of UDPG pyrophosphorylase (UGP) and phosphoglucose 
      isomerase (GP1), which is involved in the conversion of UDP-glucose to 
      glucose-6-phosphate. The phosphorus transport pathway of soybean roots was then 
      affected, which promoted phosphorus allocation to UTP and glucose-6-phosphate. 
      Additionally, low-phosphorus stress hastened glycolysis in the soybean roots and 
      inhibited the synthesis of malic acid, thereby promoting the transport of 
      phosphorus in the roots. In addition, low-phosphorus stress inhibited the 
      synthesis of fructose, mannose, and mannose-1-phosphate and the synthesis of 
      other enzymes involved in phosphorus transport as well as invertase, thereby 
      inhibiting the transport and synthesis of several organic phosphorus-containing 
      compounds.
CI  - Copyright (c) 2022 Li, Xu, Li, Lyu, Li, Wang, Wang, Ma and Yan.
FAU - Li, Hongyu
AU  - Li H
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
FAU - Xu, Letian
AU  - Xu L
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
FAU - Li, Jiaxin
AU  - Li J
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
FAU - Lyu, Xiaochen
AU  - Lyu X
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
FAU - Li, Sha
AU  - Li S
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
AD  - College of Resources and Environment, Northeast Agricultural University, Harbin, 
      China.
FAU - Wang, Chang
AU  - Wang C
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
FAU - Wang, Xuelai
AU  - Wang X
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
FAU - Ma, Chunmei
AU  - Ma C
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
FAU - Yan, Chao
AU  - Yan C
AD  - College of Agriculture, Northeast Agricultural University, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20220902
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC9478169
OTO - NOTNLM
OT  - low phosphorus stress
OT  - metabonomics
OT  - phosphorus transport
OT  - proteomics
OT  - transcriptomics
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/09/20 06:00
MHDA- 2022/09/20 06:01
PMCR- 2022/01/01
CRDT- 2022/09/19 04:29
PHST- 2022/07/12 00:00 [received]
PHST- 2022/08/04 00:00 [accepted]
PHST- 2022/09/19 04:29 [entrez]
PHST- 2022/09/20 06:00 [pubmed]
PHST- 2022/09/20 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2022.992036 [doi]
PST - epublish
SO  - Front Plant Sci. 2022 Sep 2;13:992036. doi: 10.3389/fpls.2022.992036. eCollection 
      2022.