PMID- 36122679
OWN - NLM
STAT- MEDLINE
DCOM- 20221108
LR  - 20221116
IS  - 1873-6300 (Electronic)
IS  - 0891-0618 (Linking)
VI  - 125
DP  - 2022 Nov
TI  - Transient receptor potential melastatin-7 in the rat dorsal root ganglion.
PG  - 102163
LID - S0891-0618(22)00093-X [pii]
LID - 10.1016/j.jchemneu.2022.102163 [doi]
AB  - AIMS: Transient receptor potential melastatin-7 (TRPM7) is a selective cation 
      permeable channel which plays important roles in cellular and developmental 
      biology such as cell proliferation, survival, differentiation and migration. This 
      channel is also known to be necessary for transmitter release in the peripheral 
      nervous system. In this study, immunohistochemistry for TRPM7 was conducted in 
      the rat lumbar dorsal root ganglion (DRG). METHODS: Triple immunofluorescence 
      methods were used to demonstrate distribution of TRPM7 and its relationship to 
      other TRP channels in the DRG. Retrograde tracing and double immunofluorescence 
      methods were also performed to know peripheral targets of DRG neurons containing 
      TRPM7 and TRP vanilloid 1 (TRPV1). In addition, transection of the sciatic nerve 
      was conducted to demonstrate an effect of the nerve injury on TRPM7expression in 
      the DRG. RESULTS: TRPM7-immunoreactivity was expressed by 53.9% of sensory 
      neurons in the 4th lumbar DRG. TRPM7-immunoreactive (-IR) DRG neurons mostly had 
      small (<600 microm(2)) and medium-sized (600-1200 microm(2)) cell bodies. By triple and 
      double immunofluorescence methods, approximately 70% of TRPM7-IR DRG neurons 
      contained TRPV1-immunoreactivity. Although the number of DRG neurons 
      co-expressing TRPM7 and TRPM8 was small in the DRG, almost all of TRPM8-IR DRG 
      neurons co-expressed TRPM7-immunoreactivity. By combination of retrograde tracing 
      method and immunohistochemistry, TRPM7 was expressed by half of DRG neurons 
      innervating the plantar skin (61.9%) and gastrocnemius muscle (51.2%), and 79.6% 
      of DRG neurons innervating the periosteum. Co-expression of TRPM7 and TRPV1 among 
      periosteum DRG neurons (75.7%) was more abundant than among cutaneous (53.2%) and 
      muscular (40.4%) DRG neurons. DRG neurons which co-expressed these ion channels 
      in the periosteum had smaller cell bodies compared to the skin and muscle. In 
      addition, the sciatic nerve transection decreased the number of TRPM7-IR neurons 
      in the DRG (approximately 60% reduction). The RT-qPCR analysis also demonstrated 
      reduction of TRPM7 mRNA in the injured DRG. CONCLUSION: The present study 
      suggests that TRPM7 is mainly located in small nociceptors in the DRG. The 
      content of TRPM7 in DRG neurons is probably different among their peripheral 
      targets. TRPM7 in DRG neurons may be able to respond to noxious stimulation from 
      their peripheral tissues. The nerve injury can decrease the level of TRPM7 mRNA 
      and protein in DRG neurons.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Yajima, Takehiro
AU  - Yajima T
AD  - Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku 
      University, Sendai 980-8575, Japan.
FAU - Sato, Tadasu
AU  - Sato T
AD  - Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku 
      University, Sendai 980-8575, Japan. Electronic address: tadasu@dent.tohoku.ac.jp.
FAU - Hosokawa, Hiroshi
AU  - Hosokawa H
AD  - Department of Intelligence Science and Technology, Graduate School of 
      Informatics, Kyoto University, Kyoto 606-8501, Japan.
FAU - Kondo, Teruyoshi
AU  - Kondo T
AD  - Department of Animal Pharmaceutical Sciences, School of Pharmaceutical Sciences, 
      Kyushu University of Health and Welfare, Nobeoka 882-8508, Japan.
FAU - Ichikawa, Hiroyuki
AU  - Ichikawa H
AD  - Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku 
      University, Sendai 980-8575, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220917
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - 0 (TRPM Cation Channels)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.1 (Trpm7 protein, rat)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Ganglia, Spinal/metabolism
MH  - *TRPM Cation Channels/metabolism
MH  - TRPV Cation Channels/metabolism
MH  - Sensory Receptor Cells/metabolism
MH  - RNA, Messenger/metabolism
OTO - NOTNLM
OT  - Dorsal root ganglion
OT  - Immunohistochemistry
OT  - TRPM7
OT  - TRPM8
OT  - TRPV1
OT  - TRPV2
COIS- Conflict of interest The authors declare that they have no conflict of interest.
EDAT- 2022/09/20 06:00
MHDA- 2022/11/09 06:00
CRDT- 2022/09/19 19:24
PHST- 2021/12/16 00:00 [received]
PHST- 2022/09/08 00:00 [revised]
PHST- 2022/09/15 00:00 [accepted]
PHST- 2022/09/20 06:00 [pubmed]
PHST- 2022/11/09 06:00 [medline]
PHST- 2022/09/19 19:24 [entrez]
AID - S0891-0618(22)00093-X [pii]
AID - 10.1016/j.jchemneu.2022.102163 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2022 Nov;125:102163. doi: 10.1016/j.jchemneu.2022.102163. Epub 
      2022 Sep 17.