PMID- 36125713
OWN - NLM
STAT- MEDLINE
DCOM- 20220923
LR  - 20220926
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2544
DP  - 2022
TI  - Co-culture Model for Hepatitis B Virus Infection Using iPSC-Derived Liver 
      Progenitor Cells and Liver Sinusoidal Endothelial Cells.
PG  - 107-117
LID - 10.1007/978-1-0716-2557-6_7 [doi]
AB  - In order to understand the complicated life cycle of hepatitis B virus (HBV), it 
      is necessary to develop a co-culture system for HBV infection using various liver 
      cells. We have already developed culture systems to generate liver progenitor 
      cells (LPCs), hepatocytes, and liver sinusoidal endothelial cells (LSECs) from 
      human induced pluripotent cells (iPSCs). Using those cells, we established a 
      co-culture system to study HBV infection and replication in vitro.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Himeno, Misao
AU  - Himeno M
AD  - Laboratory of Cell Growth and Differentiation, Institute for Quantitative 
      Biosciences, The University of Tokyo, Tokyo, Japan. mhimeno@iqb.u-tokyo.ac.jp.
FAU - Chen, Shin-Wei
AU  - Chen SW
AD  - Laboratory of Cell Growth and Differentiation, Institute for Quantitative 
      Biosciences, The University of Tokyo, Tokyo, Japan. cwei207@gmail.com.
FAU - Kido, Taketomo
AU  - Kido T
AD  - Laboratory of Cell Growth and Differentiation, Institute for Quantitative 
      Biosciences, The University of Tokyo, Tokyo, Japan. kido@iqb.u-tokyo.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Coculture Techniques
MH  - Endothelial Cells
MH  - *Hepatitis B
MH  - Hepatitis B virus
MH  - Hepatocytes
MH  - Humans
MH  - *Induced Pluripotent Stem Cells
OTO - NOTNLM
OT  - Co-culture system
OT  - Hepatic stellate cells
OT  - Hepatitis B virus
OT  - Liver
OT  - Liver sinusoidal endothelial cells
EDAT- 2022/09/21 06:00
MHDA- 2022/09/24 06:00
CRDT- 2022/09/20 11:21
PHST- 2022/09/20 11:21 [entrez]
PHST- 2022/09/21 06:00 [pubmed]
PHST- 2022/09/24 06:00 [medline]
AID - 10.1007/978-1-0716-2557-6_7 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2544:107-117. doi: 10.1007/978-1-0716-2557-6_7.