PMID- 36126873 OWN - NLM STAT- MEDLINE DCOM- 20221101 LR - 20221103 IS - 1873-2747 (Electronic) IS - 0361-9230 (Linking) VI - 190 DP - 2022 Nov TI - Inhibitory effects of antibiotic-induced gut microbiota depletion on acute itch behavior in mice. PG - 50-61 LID - S0361-9230(22)00254-4 [pii] LID - 10.1016/j.brainresbull.2022.09.014 [doi] AB - BACKGROUND: The gut microbiota is known to be associated with the regulation of many neurological diseases and behaviors, including chronic pain. However, it is unclear whether the gut microbiota is critical to the itch sensation. In this study, we investigated the effects of gut microbiota depletion on acute itch. METHODS: First, an antibiotic cocktail was orally administered to deplete the gut microbiota in male C57BL/6 mice. Then, pruritogens were intradermally injected to induce acute itch behavior. In addition, antibiotic-treated mice received transplantation of fecal microbiota from untreated mice, followed by tests for acute itch. The changes in c-Fos expression in trigeminal ganglia (TG) neurons were also investigated by immunofluorescence staining. RESULTS: Our results indicated that chronic antibiotic treatment significantly reduced the diversity and richness of the gut microbiota of mice. Compared to vehicle-treated mice, antibiotic-treated mice showed reductions in acute itch behavior induced by compound 48/80, chloroquine (CQ), and serotonin (5-HT), respectively. Moreover, repositioning of microbiota reversed the reductions in acute itch behavior in antibiotic-treated mice. In addition, immunofluorescence staining revealed that antibiotic-treated mice displayed decreased c-Fos expression in ipsilateral TG compared to controls. CONCLUSIONS: Our study, for the first time, discovered that antibiotic-induced gut microbiota depletion could reduce acute itch behavior, which may be connected with decreased TG neuronal activity. CI - Copyright (c) 2022 Elsevier Inc. All rights reserved. FAU - Zhang, Qinqin AU - Zhang Q AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Li, Tingting AU - Li T AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Niu, Jiandong AU - Niu J AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Xiao, Jian AU - Xiao J AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Zhang, Mengna AU - Zhang M AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Zhang, Run AU - Zhang R AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Chen, Dan AU - Chen D AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Shi, Yonghang AU - Shi Y AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Zhang, Xiaodi AU - Zhang X AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Hu, Xuanran AU - Hu X AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Yu, Bowen AU - Yu B AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. FAU - Feng, Jie AU - Feng J AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. Electronic address: jfeng@lzu.edu.cn. FAU - Fang, Quan AU - Fang Q AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, and State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou, China. Electronic address: fangq@lzu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220917 PL - United States TA - Brain Res Bull JT - Brain research bulletin JID - 7605818 RN - 0 (Anti-Bacterial Agents) RN - 333DO1RDJY (Serotonin) SB - IM MH - Animals MH - Mice MH - Male MH - *Gastrointestinal Microbiome/physiology MH - Mice, Inbred C57BL MH - Anti-Bacterial Agents/pharmacology MH - Pruritus/chemically induced/drug therapy MH - Serotonin/pharmacology OTO - NOTNLM OT - Acute itch OT - Antibiotic OT - C-Fos OT - Fecal microbiota transplantation OT - Gut microbiota COIS- Conflict of interest The authors declare that they have no conflict of interest. EDAT- 2022/09/21 06:00 MHDA- 2022/11/02 06:00 CRDT- 2022/09/20 19:26 PHST- 2022/05/13 00:00 [received] PHST- 2022/09/07 00:00 [revised] PHST- 2022/09/16 00:00 [accepted] PHST- 2022/09/21 06:00 [pubmed] PHST- 2022/11/02 06:00 [medline] PHST- 2022/09/20 19:26 [entrez] AID - S0361-9230(22)00254-4 [pii] AID - 10.1016/j.brainresbull.2022.09.014 [doi] PST - ppublish SO - Brain Res Bull. 2022 Nov;190:50-61. doi: 10.1016/j.brainresbull.2022.09.014. Epub 2022 Sep 17.