PMID- 36128996
OWN - NLM
STAT- MEDLINE
DCOM- 20230209
LR  - 20240202
IS  - 1941-2444 (Electronic)
IS  - 0148-6071 (Print)
IS  - 0148-6071 (Linking)
VI  - 47
IP  - 2
DP  - 2023 Feb
TI  - Regulation of skeletal muscle protein synthesis in the preterm pig by 
      intermittent leucine pulses during continuous parenteral feeding.
PG  - 276-286
LID - 10.1002/jpen.2450 [doi]
AB  - BACKGROUND: Extrauterine growth restriction is a common complication of preterm 
      birth. Leucine (Leu) is an agonist for the mechanistic target of rapamycin (mTOR) 
      complex 1 (mTORC1) signaling pathway that regulates translation initiation and 
      protein synthesis in skeletal muscle. Previously, we showed that intermittent 
      intravenous pulses of Leu to neonatal pigs born at term receiving continuous 
      enteral nutrition increases muscle protein synthesis and lean mass accretion. Our 
      objective was to determine the impact of intermittent intravenous pulses of Leu 
      on muscle protein anabolism in preterm neonatal pigs administered continuous 
      parenteral nutrition. METHODS: Following preterm delivery (on day 105 of 115 
      gestation), pigs were fitted with umbilical artery and jugular vein catheters and 
      provided continuous parenteral nutrition. Four days after birth, pigs were 
      assigned to receive intermittent Leu (1600 micromol kg(-1)  h(-1) ; n = 8) or alanine 
      (1600 micromol kg(-1)  h(-1) ; n = 8) parenteral pulses every 4 h for 28 h. Anabolic 
      signaling and fractional protein synthesis were determined in skeletal muscle. 
      RESULTS: Leu concentration in the longissimus dorsi and gastrocnemius muscles 
      increased in the leucine (LEU) group compared with the alanine (ALA) group 
      (P < 0.0001). Despite the Leu-induced disruption of the Sestrin2.GATOR2 complex, 
      which inhibits mTORC1 activation, in these muscles (P < 0.01), the abundance of 
      mTOR.RagA and mTOR.RagC was not different. Accordingly, mTORC1-dependent 
      activation of 4EBP1, S6K1, eIF4E.eIF4G, and protein synthesis were not different 
      in any muscle between the LEU and ALA groups. CONCLUSION: Intermittent pulses of 
      Leu do not enhance muscle protein anabolism in preterm pigs supplied continuous 
      parenteral nutrition.
CI  - (c) 2022 American Society for Parenteral and Enteral Nutrition.
FAU - Rudar, Marko
AU  - Rudar M
AD  - Department of Animal Sciences, Auburn University, Auburn, Alabama, USA.
FAU - Suryawan, Agus
AU  - Suryawan A
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Nguyen, Hanh V
AU  - Nguyen HV
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Chacko, Shaji K
AU  - Chacko SK
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Vonderohe, Caitlin
AU  - Vonderohe C
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Stoll, Barbara
AU  - Stoll B
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Burrin, Douglas G
AU  - Burrin DG
AUID- ORCID: 0000-0002-8792-3339
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Fiorotto, Marta L
AU  - Fiorotto ML
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
FAU - Davis, Teresa A
AU  - Davis TA
AUID- ORCID: 0000-0002-4610-9795
AD  - USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, 
      Houston, Texas, USA.
LA  - eng
GR  - R01 HD099080/HD/NICHD NIH HHS/United States
GR  - R01 HD072891/HD/NICHD NIH HHS/United States
GR  - R01 HD085573/HD/NICHD NIH HHS/United States
GR  - R01 AR044474/AR/NIAMS NIH HHS/United States
GR  - T32DK007664/DK/NIDDK NIH HHS/United States
GR  - T32 DK007664/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20221010
PL  - United States
TA  - JPEN J Parenter Enteral Nutr
JT  - JPEN. Journal of parenteral and enteral nutrition
JID - 7804134
RN  - GMW67QNF9C (Leucine)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - OF5P57N2ZX (Alanine)
RN  - 0 (Muscle Proteins)
SB  - IM
MH  - Infant, Newborn
MH  - Female
MH  - Humans
MH  - Animals
MH  - Swine
MH  - Leucine/metabolism/pharmacology
MH  - Animals, Newborn
MH  - *Premature Birth/metabolism
MH  - Muscle, Skeletal/metabolism
MH  - TOR Serine-Threonine Kinases
MH  - Mechanistic Target of Rapamycin Complex 1/metabolism
MH  - Alanine/metabolism
MH  - Muscle Proteins/metabolism
MH  - Parenteral Nutrition
MH  - Protein Biosynthesis
PMC - PMC10621874
MID - NIHMS1940859
OTO - NOTNLM
OT  - mTOR
OT  - parenteral nutrition
OT  - pig
OT  - prematurity
OT  - protein synthesis
OT  - skeletal muscle
COIS- CONFLICT OF INTEREST None declared.
EDAT- 2022/09/22 06:00
MHDA- 2023/02/10 06:00
PMCR- 2024/02/01
CRDT- 2022/09/21 06:13
PHST- 2022/08/31 00:00 [revised]
PHST- 2022/06/30 00:00 [received]
PHST- 2022/09/15 00:00 [accepted]
PHST- 2022/09/22 06:00 [pubmed]
PHST- 2023/02/10 06:00 [medline]
PHST- 2022/09/21 06:13 [entrez]
PHST- 2024/02/01 00:00 [pmc-release]
AID - 10.1002/jpen.2450 [doi]
PST - ppublish
SO  - JPEN J Parenter Enteral Nutr. 2023 Feb;47(2):276-286. doi: 10.1002/jpen.2450. 
      Epub 2022 Oct 10.