PMID- 36130878
OWN - NLM
STAT- MEDLINE
DCOM- 20221124
LR  - 20221124
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 86
IP  - 12
DP  - 2022 Nov 23
TI  - Blood-derived exosomes released after the oral administration of heat-killed 
      Enterococcus faecalis activate immunity.
PG  - 1699-1704
LID - 10.1093/bbb/zbac158 [doi]
AB  - We aimed to examine the mechanism of heat-killed Enterococcus faecalis (HkEf) 
      immunostimulatory effect when orally administered. Immunocompetent splenocytes 
      from mice orally administered HkEf were assessed using flow cytometry. 
      Immunocompetent cells were determined by culturing splenocytes with serum or 
      blood derived-exosomes. In vitro studies evaluated the reaction between mouse 
      splenocytes and exosomes purified and isolated from mice bone marrow-derived 
      macrophages (BMDMs) treated with HkEf. Levels of dendritic cells, red pulp 
      macrophages, and inactive NK cells were significantly higher in the HkEf-treated 
      group. Red pulp macrophages and inactive NK cells were increased in splenocytes 
      cultured with blood-derived exosomes from mice administered HkEf. Further, mouse 
      splenocytes cultured with the HkEf-stimulated BMDMs-derived exosomes group had 
      significantly higher levels of red pulp macrophages. Thus, HkEf was involved in 
      host immunostimulation and exosomes were identified as mediators in immune 
      response signaling. Further verification of the mechanism would be needed to fill 
      in the gap between the present results and conclusions.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of Japan 
      Society for Bioscience, Biotechnology, and Agrochemistry.
FAU - Matsuo, Tomoe
AU  - Matsuo T
AD  - Academic Division, NUTRI Co., Ltd., 4-1-1 Toranomon, Minato-ku, Tokyo, Japan.
FAU - Nakao, Koji
AU  - Nakao K
AD  - Academic Division, NUTRI Co., Ltd., 4-1-1 Toranomon, Minato-ku, Tokyo, Japan.
FAU - Hara, Kosuke
AU  - Hara K
AD  - Academic Division, NUTRI Co., Ltd., 4-1-1 Toranomon, Minato-ku, Tokyo, Japan.
FAU - Kawaguchi, Susumu
AU  - Kawaguchi S
AD  - R&D Division, NUTRI Co., Ltd., 4-1-1 Toranomon, Minato-ku, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Exosomes
MH  - Enterococcus faecalis
MH  - Hot Temperature
MH  - Mice, Inbred C57BL
MH  - Administration, Oral
OTO - NOTNLM
OT  - HkEf
OT  - exosomes
OT  - macrophage
EDAT- 2022/09/22 06:00
MHDA- 2022/11/25 06:00
CRDT- 2022/09/21 22:42
PHST- 2022/05/31 00:00 [received]
PHST- 2022/09/01 00:00 [accepted]
PHST- 2022/09/22 06:00 [pubmed]
PHST- 2022/11/25 06:00 [medline]
PHST- 2022/09/21 22:42 [entrez]
AID - 6710372 [pii]
AID - 10.1093/bbb/zbac158 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2022 Nov 23;86(12):1699-1704. doi: 
      10.1093/bbb/zbac158.