PMID- 36132778
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220924
IS  - 2516-0230 (Electronic)
IS  - 2516-0230 (Linking)
VI  - 2
IP  - 9
DP  - 2020 Sep 16
TI  - Poly-d-lysine coated nanoparticles to identify pro-inflammatory macrophages.
PG  - 3849-3857
LID - 10.1039/d0na00373e [doi]
AB  - Identifying pro-inflammatory macrophages (M1) is of immense importance to 
      diagnose, monitor, and treat various pathologies. In addition, adoptive cell 
      therapies, where harvested cells are isolated, modified to express an M1-like 
      phenotype, then re-implanted to the patient, are also becoming more prevalent to 
      treat diseases such as cancer. In a step toward identifying, labeling, and 
      monitoring macrophage phenotype for adoptive cell therapies, we developed a 
      reactive oxygen species (ROS)-sensitive, gold nanoparticle (AuNP) that 
      fluorescently labels M1 macrophages. AuNPs are electrostatically coated with a 
      proteolysis resistant, fluorescein isothiocyanate-conjugated, poly-d-lysine 
      (PDL-FITC) that is susceptible to backbone cleavage by ROS. When PDL-FITC is 
      bound to AuNPs, fluorescence is quenched via a combination of nanoparticle 
      surface (NSET) and Forster resonance (FRET) energy transfer mechanisms. Upon 
      ROS-induced cleavage of PDL-FITC, up to a 7-fold change in fluorescence is 
      demonstrated. PDL-FITC AuNPs were loaded into RAW 264.7 macrophages (RAWs) and 
      primary bone marrow- derived macrophages (BMDMs) prior to in vitro polarization. 
      For both cell types, detectable differences in intracellular fluorescence were 
      observed between M1 polarized and non-stimulated (M0) control groups after 24 h 
      using both confocal imaging and flow cytometry. PDL-FITC AuNPs can potentially be 
      useful in identifying M1 macrophages within diverse cell populations and provide 
      longitudinal macrophage response data to external cues.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Hernandez, Derek S
AU  - Hernandez DS
AD  - Department of Biomedical Engineering, University of Texas at Austin Austin TX 
      78712 USA suggs@utexas.edu.
FAU - Schunk, Hattie C
AU  - Schunk HC
AUID- ORCID: 0000-0002-4768-2569
AD  - Department of Biomedical Engineering, University of Texas at Austin Austin TX 
      78712 USA suggs@utexas.edu.
AD  - McKetta Department of Chemical Engineering, University of Texas at Austin Austin 
      TX 78712 USA.
FAU - Shankar, Karan M
AU  - Shankar KM
AD  - Department of Biomedical Engineering, University of Texas at Austin Austin TX 
      78712 USA suggs@utexas.edu.
FAU - Rosales, Adrianne M
AU  - Rosales AM
AUID- ORCID: 0000-0003-0207-7661
AD  - McKetta Department of Chemical Engineering, University of Texas at Austin Austin 
      TX 78712 USA.
FAU - Suggs, Laura J
AU  - Suggs LJ
AUID- ORCID: 0000-0002-1419-8017
AD  - Department of Biomedical Engineering, University of Texas at Austin Austin TX 
      78712 USA suggs@utexas.edu.
LA  - eng
PT  - Journal Article
DEP - 20200713
PL  - England
TA  - Nanoscale Adv
JT  - Nanoscale advances
JID - 101738708
PMC - PMC9416964
COIS- There are no conflicts to declare.
EDAT- 2020/07/13 00:00
MHDA- 2020/07/13 00:01
PMCR- 2020/07/13
CRDT- 2022/09/22 03:40
PHST- 2020/05/07 00:00 [received]
PHST- 2020/07/12 00:00 [accepted]
PHST- 2022/09/22 03:40 [entrez]
PHST- 2020/07/13 00:00 [pubmed]
PHST- 2020/07/13 00:01 [medline]
PHST- 2020/07/13 00:00 [pmc-release]
AID - d0na00373e [pii]
AID - 10.1039/d0na00373e [doi]
PST - epublish
SO  - Nanoscale Adv. 2020 Jul 13;2(9):3849-3857. doi: 10.1039/d0na00373e. eCollection 
      2020 Sep 16.