PMID- 36133633
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220924
IS  - 2636-0780 (Electronic)
IS  - 2636-0772 (Print)
IS  - 2636-0772 (Linking)
VI  - 42
IP  - 5
DP  - 2022 Sep
TI  - Monitoring Cellular Immune Responses after Consumption of Selected Probiotics in 
      Immunocompromised Mice.
PG  - 903-914
LID - 10.5851/kosfa.2022.e44 [doi]
AB  - Probiotics are currently considered as one of tools to modulate immune responses 
      under specific clinical conditions. The purpose of this study was to evaluate 
      whether oral administration of three different probiotics (Lactiplantibacillus 
      plantarum CJLP243, CJW55-10, and CJLP475) could evoke a cell-mediated immunity in 
      immunodeficient mice. Before conducting in vivo experiments, we examined the in 
      vitro potency of these probiotics for macrophage activation. After co-culture 
      with these probiotics, bone marrow derived macrophages (BMDMs) produced 
      significant amounts of proinflammatory cytokines including interleukin-6 (IL-6), 
      IL-12, and tumor necrosis factor-alpha (TNF-alpha). Levels of inducible nitric oxide 
      synthase (inos) and co-stimulatory molecules (CD80 and CD86) were also 
      upregulated in BMDMs after treatment with some of these probiotics. To establish 
      an immunocompromised animal model, we intraperitoneally injected mice with 
      cyclophosphamide on day 0 and again on day 2. Starting day 3, we orally 
      administered probiotics every day for the last 15 d. After sacrificing 
      experimental mice on day 18, splenocytes were isolated and co-cultured with these 
      probiotics for 3 d to measure levels of several cytokines and immune cell 
      proliferation. Results clearly indicated that the consumption of all three 
      probiotic strains promoted secretion of interferon-gamma (IFN-gamma), IL-1beta, IL-6, IL-12, 
      and TNF-alpha. NK cell cytotoxicity and proliferation of immune cells were also 
      increased. Taken together, our data strongly suggest that consumption of some 
      probiotics might induce cell-mediated immune responses in immunocompromised mice.
CI  - (c) Korean Society for Food Science of Animal Resources.
FAU - Kang, Seok-Jin
AU  - Kang SJ
AUID- ORCID: 0000-0002-9701-8690
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
AD  - Institute of Animal Molecular Biotechnology, Korea University, Seoul 02841, 
      Korea.
FAU - Yang, Jun
AU  - Yang J
AUID- ORCID: 0000-0002-9260-7875
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
FAU - Lee, Na-Young
AU  - Lee NY
AUID- ORCID: 0000-0001-6053-3528
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
FAU - Lee, Chang-Hee
AU  - Lee CH
AUID- ORCID: 0000-0002-4424-7203
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
AD  - Institute of Animal Molecular Biotechnology, Korea University, Seoul 02841, 
      Korea.
FAU - Park, In-Byung
AU  - Park IB
AUID- ORCID: 0000-0002-8646-8709
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
AD  - Institute of Animal Molecular Biotechnology, Korea University, Seoul 02841, 
      Korea.
FAU - Park, Si-Won
AU  - Park SW
AUID- ORCID: 0000-0002-1597-9683
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
FAU - Lee, Hyeon Jeong
AU  - Lee HJ
AUID- ORCID: 0000-0003-0277-7377
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
FAU - Park, Hae-Won
AU  - Park HW
AUID- ORCID: 0000-0001-5915-2154
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
FAU - Yun, Hyun Sun
AU  - Yun HS
AUID- ORCID: 0000-0002-4655-9462
AD  - CJ CheilJedang Corporation, Suwon 16495, Korea.
FAU - Chun, Taehoon
AU  - Chun T
AUID- ORCID: 0000-0002-5940-8620
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul 02841, Korea.
LA  - eng
PT  - Journal Article
DEP - 20220901
PL  - Korea (South)
TA  - Food Sci Anim Resour
JT  - Food science of animal resources
JID - 101742126
PMC - PMC9478974
OTO - NOTNLM
OT  - animal model
OT  - immune response
OT  - probiotics
COIS- Yun HS is currently employed by the CJ CheilJedang Corporation, Korea. None of 
      the other authors had any conflict of interests.
EDAT- 2022/09/23 06:00
MHDA- 2022/09/23 06:01
PMCR- 2022/09/01
CRDT- 2022/09/22 03:59
PHST- 2022/06/23 00:00 [received]
PHST- 2022/08/02 00:00 [revised]
PHST- 2022/08/11 00:00 [accepted]
PHST- 2022/09/22 03:59 [entrez]
PHST- 2022/09/23 06:00 [pubmed]
PHST- 2022/09/23 06:01 [medline]
PHST- 2022/09/01 00:00 [pmc-release]
AID - kosfa-42-5-903 [pii]
AID - 10.5851/kosfa.2022.e44 [doi]
PST - ppublish
SO  - Food Sci Anim Resour. 2022 Sep;42(5):903-914. doi: 10.5851/kosfa.2022.e44. Epub 
      2022 Sep 1.