PMID- 36134856
OWN - NLM
STAT- MEDLINE
DCOM- 20220923
LR  - 20221007
IS  - 1678-7765 (Electronic)
IS  - 1678-7757 (Print)
IS  - 1678-7757 (Linking)
VI  - 30
DP  - 2022
TI  - Implications of lysyl oxidase-like protein 3 expression in the periodontium of 
      diabetic rats.
PG  - e20220176
LID - S1678-77572022000100438 [pii]
LID - 10.1590/1678-7757-2022-0176 [doi]
LID - e20220176
AB  - OBJECTIVES: Diabetes has been strongly associated with periodontal diseases. The 
      periodontal ligament (PDL) has an abundant extracellular matrix (ECM). Lysyl 
      oxidases (LOXs) are closely associated with various diseases caused by abnormal 
      ECM functions, however, the role of LOXs in periodontal diseases induced by 
      diabetes remains unclear. METHODOLOGY: In this study, 8-week-old Zucker diabetic 
      fatty rats were used to establish a type 2 diabetes mellitus (T2DM) model. After 
      9 and 16 weeks, hematoxylin and eosin (H&E), Masson's trichrome, and 
      immunohistochemical staining were performed. RESULTS: After 9 weeks, loose 
      collagen fibers were found in the interradicular area of the diabetic group, in 
      opposition to the control group. There were no significant differences in LOX 
      expression between the diabetic and control groups (p>0.05). However, after 16 
      weeks, the diabetic group presented a disordered arrangement of the PDL, showing 
      decreased collagen content and significantly increased lysyl oxidase-like protein 
      3 (LOXL3) expression when compared with the control group (p<0.05). This suggests 
      that LOXL3 plays a significant role in periodontal histopathological changes in 
      diabetic rats. CONCLUSION: Our study showed elevated LOXL3 expression in the PDL 
      of diabetic rats after 16 weeks, suggesting that LOXL3 may be involved in the 
      occurrence and development of periodontal histopathological changes in diabetic 
      rats. LOXL3 could be further used as an indicator for the early diagnosis of 
      diabetic periodontitis in T2DM patients in clinical settings.
FAU - Huang, Li
AU  - Huang L
AUID- ORCID: 0000-0002-2534-2926
AD  - West China Hospital of Stomatology, National Clinical Research Center for Oral 
      Diseases, Sichuan University, State Key Laboratory of Oral Diseases, Chengdu, 
      China.
AD  - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Chen, Jun
AU  - Chen J
AUID- ORCID: 0000-0003-1403-9806
AD  - West China Hospital of Stomatology, National Clinical Research Center for Oral 
      Diseases, Sichuan University, State Key Laboratory of Oral Diseases, Chengdu, 
      China.
AD  - Northwest Minzu University, Key Laboratory of Oral Diseases of Gansu Province/Key 
      Laboratory of Stomatology of State Ethnic Affairs Commission, Lanzhou, Gansu, 
      China.
FAU - Zuo, Yuling
AU  - Zuo Y
AUID- ORCID: 0000-0003-2084-5097
AD  - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
FAU - Li, Jinle
AU  - Li J
AUID- ORCID: 0000-0001-5305-6639
AD  - West China Hospital of Stomatology, National Clinical Research Center for Oral 
      Diseases, Sichuan University, State Key Laboratory of Oral Diseases, Chengdu, 
      China.
FAU - Yue, Yuan
AU  - Yue Y
AUID- ORCID: 0000-0002-3076-4971
AD  - West China Hospital of Stomatology, National Clinical Research Center for Oral 
      Diseases, Sichuan University, State Key Laboratory of Oral Diseases, Chengdu, 
      China.
FAU - Wang, Min
AU  - Wang M
AUID- ORCID: 0000-0003-1791-2631
AD  - West China Hospital of Stomatology, National Clinical Research Center for Oral 
      Diseases, Sichuan University, State Key Laboratory of Oral Diseases, Chengdu, 
      China.
FAU - Hao, Liang
AU  - Hao L
AUID- ORCID: 0000-0001-7207-9131
AD  - West China Hospital of Stomatology, National Clinical Research Center for Oral 
      Diseases, Sichuan University, State Key Laboratory of Oral Diseases, Chengdu, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220919
PL  - Brazil
TA  - J Appl Oral Sci
JT  - Journal of applied oral science : revista FOB
JID - 101189774
RN  - 9007-34-5 (Collagen)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.13 (Protein-Lysine 6-Oxidase)
RN  - TDQ283MPCW (Eosine Yellowish-(YS))
RN  - YKM8PY2Z55 (Hematoxylin)
SB  - IM
MH  - Amino Acid Oxidoreductases/*metabolism
MH  - Animals
MH  - Collagen
MH  - *Diabetes Mellitus, Experimental
MH  - *Diabetes Mellitus, Type 2/complications/metabolism
MH  - Eosine Yellowish-(YS)/metabolism
MH  - Hematoxylin/metabolism
MH  - *Periodontal Diseases
MH  - Periodontal Ligament/metabolism
MH  - Periodontium
MH  - Protein-Lysine 6-Oxidase/metabolism
MH  - Rats
MH  - Rats, Zucker
PMC - PMC9529203
COIS- Conflict of interest The authors declare no potential conflict of interest 
      regarding the authorship and/or publication of this article.
EDAT- 2022/09/23 06:00
MHDA- 2022/09/24 06:00
PMCR- 2022/09/19
CRDT- 2022/09/22 07:37
PHST- 2022/05/24 00:00 [received]
PHST- 2022/07/18 00:00 [accepted]
PHST- 2022/09/22 07:37 [entrez]
PHST- 2022/09/23 06:00 [pubmed]
PHST- 2022/09/24 06:00 [medline]
PHST- 2022/09/19 00:00 [pmc-release]
AID - S1678-77572022000100438 [pii]
AID - 10.1590/1678-7757-2022-0176 [doi]
PST - epublish
SO  - J Appl Oral Sci. 2022 Sep 19;30:e20220176. doi: 10.1590/1678-7757-2022-0176. 
      eCollection 2022.