PMID- 36139733
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220928
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 11
IP  - 9
DP  - 2022 Aug 26
TI  - Gold Nanoparticles Inhibit Steroid-Insensitive Asthma in Mice Preserving Histone 
      Deacetylase 2 and NRF2 Pathways.
LID - 10.3390/antiox11091659 [doi]
LID - 1659
AB  - BACKGROUND: Gold nanoparticles (AuNPs) can inhibit pivotal pathological changes 
      in experimental asthma, but their effect on steroid-insensitive asthma is 
      unclear. The current study assessed the effectiveness of nebulized AuNPs in a 
      murine model of glucocorticoid (GC)-resistant asthma. METHODS: A/J mice were 
      sensitized and subjected to intranasal instillations of ovalbumin (OVA) once a 
      week for nine weeks. Two weeks after starting allergen stimulations, mice were 
      subjected to Budesonide or AuNP nebulization 1 h before stimuli. Analyses were 
      carried out 24 h after the last provocation. RESULTS: We found that mice 
      challenged with OVA had airway hyperreactivity, eosinophil, and neutrophil 
      infiltrates in the lung, concomitantly with peribronchiolar fibrosis, mucus 
      production, and pro-inflammatory cytokine generation compared to sham-challenged 
      mice. These changes were inhibited in mice treated with AuNPs, but not 
      Budesonide. In the GC-resistant asthmatic mice, oxidative stress was established, 
      marked by a reduction in nuclear factor erythroid 2-related factor 2 (NRF2) 
      levels and catalase activity, accompanied by elevated values of thiobarbituric 
      acid reactive substances (TBARS), phosphoinositide 3-kinases delta (PI3Kdelta) 
      expression, as well as a reduction in the nuclear expression of histone 
      deacetylase 2 (HDAC2) in the lung tissue, all of which sensitive to AuNPs but not 
      Budesonide treatment. CONCLUSION: These findings suggest that AuNPs can improve 
      GC-insensitive asthma by preserving HDAC2 and NRF2.
FAU - Serra, Magda F
AU  - Serra MF
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Cotias, Amanda C
AU  - Cotias AC
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Pimentel, Andreza S
AU  - Pimentel AS
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Arantes, Ana Carolina S de
AU  - Arantes ACS
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Pires, Ana Lucia A
AU  - Pires ALA
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Lanzetti, Manuella
AU  - Lanzetti M
AUID- ORCID: 0000-0003-3913-608X
AD  - Institute of Biomedical Science, Federal University of Rio de Janeiro, Rio de 
      Janeiro 21941-902, RJ, Brazil.
FAU - Hickmann, Jandir M
AU  - Hickmann JM
AUID- ORCID: 0000-0003-0310-4190
AD  - Institute of Physics, Federal University of Rio Grande do Sul, Porto Alegre 
      91509-900, RS, Brazil.
FAU - Barreto, Emiliano
AU  - Barreto E
AUID- ORCID: 0000-0002-4956-5075
AD  - Laboratory of Cell Biology, Federal University of Alagoas, Maceio 50072-900, AL, 
      Brazil.
FAU - Carvalho, Vinicius F
AU  - Carvalho VF
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Silva, Patricia M R E
AU  - Silva PMRE
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Cordeiro, Renato S B
AU  - Cordeiro RSB
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
FAU - Martins, Marco Aurelio
AU  - Martins MA
AD  - Laboratory of Inflammation, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio 
      de Janeiro 21040-360, RJ, Brazil.
LA  - eng
GR  - E-26/010.000983/2019/FAPERJ/
PT  - Journal Article
DEP - 20220826
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC9495660
OTO - NOTNLM
OT  - HDAC2
OT  - NRF2
OT  - gold nanoparticle
OT  - lung inflammation
OT  - oxidative imbalance
OT  - steroid-insensitive asthma
COIS- The authors declare no competing financial interests or personal relationships 
      that could have influenced the work reported in this paper.
EDAT- 2022/09/24 06:00
MHDA- 2022/09/24 06:01
PMCR- 2022/08/26
CRDT- 2022/09/23 01:08
PHST- 2022/07/01 00:00 [received]
PHST- 2022/08/19 00:00 [revised]
PHST- 2022/08/21 00:00 [accepted]
PHST- 2022/09/23 01:08 [entrez]
PHST- 2022/09/24 06:00 [pubmed]
PHST- 2022/09/24 06:01 [medline]
PHST- 2022/08/26 00:00 [pmc-release]
AID - antiox11091659 [pii]
AID - antioxidants-11-01659 [pii]
AID - 10.3390/antiox11091659 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2022 Aug 26;11(9):1659. doi: 10.3390/antiox11091659.