PMID- 36139749 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220928 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 11 IP - 9 DP - 2022 Aug 27 TI - Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients. LID - 10.3390/antiox11091675 [doi] LID - 1675 AB - The development of type 2 diabetes mellitus (T2DM) vascular complications (VCs) is associated with oxidative stress and chronic inflammation and can result in endothelial dysfunctions. Circulating microRNAs play an important role in epigenetic regulation of the etiology of T2DM. We studied 30 healthy volunteers, 26 T2DM patients with no complications, and 26 T2DM patients with VCs, to look for new biomarkers indicating a risk of developing VCs in T2DM patients. Peripheral blood samples were used to determine redox state, by measuring the endogenous antioxidant defense system (superoxide dismutase, SOD; catalase, CAT; glutathione reductase, GRd; glutathione peroxidase, GPx; and glucose-6-phosphate dehydrogenase, G6DP) and markers of oxidative damage (advanced oxidation protein products, AOPP; lipid peroxidation, LPO). Additionally, inflammatory marker levels (IL-1, IL-6, IL-18, and TNF-alpha), c-miR-21, and c-miR-126 expression were analyzed. T2DM patients showed the highest oxidative damage with increased GSSG/GSH ratios, LPO, and AOPP levels. In both diabetic groups, we found that diminished SOD activity was accompanied by increased CAT and decreased GRd and G6PD activities. Diabetic patients presented with increased relative expression of c-miR-21 and decreased relative expression of c-miR-126. Overall, c-miR-21, SOD, CAT, and IL-6 had high predictive values for diabetes diagnoses. Finally, our data demonstrated that IL-6 exhibited predictive value for VC development in the studied population. Moreover, c-miR-21 and c-miR-126, along with GPx and AOPP levels, should be considered possible markers for VC development in future studies. FAU - Lopez-Armas, Gabriela C AU - Lopez-Armas GC AUID- ORCID: 0000-0001-8571-8764 AD - Departamento de Investigacion y Extension, Centro de Ensenanza Tecnica Industrial, C. Nueva Escocia 1885, Guadalajara 44638, Mexico. FAU - Yessenbekova, Arailym AU - Yessenbekova A AUID- ORCID: 0000-0002-0706-6614 AD - Department of Biophysics, Biomedicine and Neuroscience, Al-Farabi Kazakh National University, Al-Farabi Av. 71, Almaty 050040, Kazakhstan. FAU - Gonzalez-Castaneda, Rocio E AU - Gonzalez-Castaneda RE AD - Laboratorio de Microscopia de Alta Resolucion, Departamento de Neurociencias, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Sierra Mojada 950, Guadalajara 44340, Mexico. FAU - Arellano-Arteaga, Kevin J AU - Arellano-Arteaga KJ AD - Division de Medicina Interna, Nuevo Hospital Civil Juan I. Menchaca, Universidad de Guadalajara, Salvador Quevedo y Subieta 750, Guadalajara 44340, Mexico. FAU - Guerra-Librero, Ana AU - Guerra-Librero A AUID- ORCID: 0000-0002-6243-6593 AD - Centro de Investigacion Biomedica en Red Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Investigacion Biosanitaria de Granada (Ibs), 18016 Granada, Spain. AD - Centro de Investigacion Biomedica, Instituto de Biotecnologia, Parque Tecnologico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain. FAU - Ablaikhanova, Nurzhanyat AU - Ablaikhanova N AUID- ORCID: 0000-0001-7288-1917 AD - Department of Biophysics, Biomedicine and Neuroscience, Al-Farabi Kazakh National University, Al-Farabi Av. 71, Almaty 050040, Kazakhstan. FAU - Florido, Javier AU - Florido J AD - Centro de Investigacion Biomedica, Instituto de Biotecnologia, Parque Tecnologico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain. FAU - Escames, Germaine AU - Escames G AUID- ORCID: 0000-0003-1256-7656 AD - Centro de Investigacion Biomedica en Red Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Investigacion Biosanitaria de Granada (Ibs), 18016 Granada, Spain. AD - Centro de Investigacion Biomedica, Instituto de Biotecnologia, Parque Tecnologico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain. AD - Department of Physiology, Faculty of Medicine, University of Granada, 18016 Granada, Spain. FAU - Acuna-Castroviejo, Dario AU - Acuna-Castroviejo D AUID- ORCID: 0000-0002-9680-1560 AD - Centro de Investigacion Biomedica en Red Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Investigacion Biosanitaria de Granada (Ibs), 18016 Granada, Spain. AD - Centro de Investigacion Biomedica, Instituto de Biotecnologia, Parque Tecnologico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain. AD - Department of Physiology, Faculty of Medicine, University of Granada, 18016 Granada, Spain. FAU - Rusanova, Iryna AU - Rusanova I AUID- ORCID: 0000-0002-9034-7600 AD - Centro de Investigacion Biomedica en Red Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Investigacion Biosanitaria de Granada (Ibs), 18016 Granada, Spain. AD - Centro de Investigacion Biomedica, Instituto de Biotecnologia, Parque Tecnologico de Ciencias de la Salud, Universidad de Granada, 18016 Granada, Spain. AD - Department of Biochemistry and Molecular Biology I, Faculty of Science, University of Granada, 18019 Granada, Spain. LA - eng GR - Plan Propio 25/11/2019/University of Granada/ GR - ERP-2021/Fundacion Eugenio Rodriguez Pascual/ GR - CB16-10-00238/Centro de Investigacion Biomedica en Red de Fragilidad y Envejecimiento Saludable/ PT - Journal Article DEP - 20220827 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC9495876 OTO - NOTNLM OT - IL-6 OT - circulating miR-21 OT - miR-126 OT - oxidative stress markers OT - type 2 diabetes mellitus OT - vascular complications COIS- The authors declare no conflict of interest. EDAT- 2022/09/24 06:00 MHDA- 2022/09/24 06:01 PMCR- 2022/08/27 CRDT- 2022/09/23 01:08 PHST- 2022/07/31 00:00 [received] PHST- 2022/08/16 00:00 [revised] PHST- 2022/08/19 00:00 [accepted] PHST- 2022/09/23 01:08 [entrez] PHST- 2022/09/24 06:00 [pubmed] PHST- 2022/09/24 06:01 [medline] PHST- 2022/08/27 00:00 [pmc-release] AID - antiox11091675 [pii] AID - antioxidants-11-01675 [pii] AID - 10.3390/antiox11091675 [doi] PST - epublish SO - Antioxidants (Basel). 2022 Aug 27;11(9):1675. doi: 10.3390/antiox11091675.