PMID- 36142334
OWN - NLM
STAT- MEDLINE
DCOM- 20220926
LR  - 20220928
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 18
DP  - 2022 Sep 9
TI  - Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Accelerate 
      Diabetic Wound Healing via Promoting M2 Macrophage Polarization, Angiogenesis, 
      and Collagen Deposition.
LID - 10.3390/ijms231810421 [doi]
LID - 10421
AB  - Some scholars have suggested that the clinical application of exosomes derived 
      from human umbilical cord mesenchymal stem cells (hucMSCs-exo) might represent a 
      novel strategy to improve diabetic wound healing. However, the mechanisms 
      underlying the effects of hucMSCs-exo on wound healing remain poorly understood. 
      This study aimed to identify the mechanism of hucMSCs-exo in treating diabetic 
      wounds. HucMSCs-exo were isolated from human umbilical cord mesenchymal stem 
      cells (hucMSCs) and subcutaneously injected into full-thickness wounds in 
      diabetic rats. Wound healing closure rates and histological analysis were 
      performed. The levels of tumor necrosis factor-alpha (TNF-alpha), macrophage mannose 
      receptor (MMR/CD206), platelet endothelial cell adhesion molecule-1 
      (PECAM-1/CD31), and vascular endothelial growth factor (VEGF) were detected by 
      immunohistochemistry. The degree of collagen deposition was examined using 
      Masson's trichrome staining. Gross evaluation of wound healing was carried out 
      from day 0 to 14 post-surgery, and the wound site was harvested for histology on 
      days 3, 7, and 14 post-wounding. HucMSCs-exo transplantation increased diabetic 
      wound healing. In vitro, hucMSCs-exo promoted the proliferation of human 
      umbilical vein endothelial cells (HUVECs) and NIH-3T3 cells. In vivo, hucMSCs-exo 
      reduced wound area and inflammatory infiltration and increased collagen fibers. 
      In addition, wound tissues in the hucMSCs-exo group had higher CD206, CD31, and 
      VEGF expressions and lower TNF-alpha levels than those in the control group on day 
      14. Our results demonstrated that hucMSCs-exo facilitated diabetic wound repair 
      by inducing anti-inflammatory macrophages and promoting angiogenesis and collagen 
      deposition.
FAU - Teng, Liping
AU  - Teng L
AD  - Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China.
FAU - Maqsood, Maria
AU  - Maqsood M
AD  - School of Life Sciences and Heath Engineering, Jiangnan University, Wuxi 214122, 
      China.
FAU - Zhu, Min
AU  - Zhu M
AD  - Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China.
FAU - Zhou, Yuting
AU  - Zhou Y
AD  - School of Life Sciences and Heath Engineering, Jiangnan University, Wuxi 214122, 
      China.
FAU - Kang, Mingzhu
AU  - Kang M
AD  - School of Life Sciences and Heath Engineering, Jiangnan University, Wuxi 214122, 
      China.
FAU - Zhou, Juan
AU  - Zhou J
AUID- ORCID: 0000-0001-9770-7229
AD  - School of Life Sciences and Heath Engineering, Jiangnan University, Wuxi 214122, 
      China.
FAU - Chen, Jinghua
AU  - Chen J
AD  - School of Life Sciences and Heath Engineering, Jiangnan University, Wuxi 214122, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220909
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Collagen/metabolism
MH  - *Diabetes Mellitus, Experimental/metabolism/therapy
MH  - *Exosomes/metabolism
MH  - Human Umbilical Vein Endothelial Cells/metabolism
MH  - Humans
MH  - Macrophages/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Mice
MH  - Neovascularization, Pathologic/metabolism
MH  - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Umbilical Cord
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - Wound Healing
PMC - PMC9498995
OTO - NOTNLM
OT  - diabetes
OT  - exosomes
OT  - human-umbilical-cord-derived mesenchymal stem cells
OT  - macrophage polarization
OT  - wound healing
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/24 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/09/09
CRDT- 2022/09/23 01:23
PHST- 2022/07/26 00:00 [received]
PHST- 2022/08/05 00:00 [revised]
PHST- 2022/08/07 00:00 [accepted]
PHST- 2022/09/23 01:23 [entrez]
PHST- 2022/09/24 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/09/09 00:00 [pmc-release]
AID - ijms231810421 [pii]
AID - ijms-23-10421 [pii]
AID - 10.3390/ijms231810421 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Sep 9;23(18):10421. doi: 10.3390/ijms231810421.