PMID- 36142468
OWN - NLM
STAT- MEDLINE
DCOM- 20220926
LR  - 20220928
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 18
DP  - 2022 Sep 12
TI  - Comparison between Immunocytochemistry, FISH and NGS for ALK and ROS1 
      Rearrangement Detection in Cytological Samples.
LID - 10.3390/ijms231810556 [doi]
LID - 10556
AB  - The detection of ROS1 and ALK rearrangements is performed for advanced-stage 
      non-small cell lung cancer. Several techniques can be used on cytological 
      samples, such as immunocytochemistry (ICC), fluorescence in situ hybridization 
      (FISH) and, more recently, next-generation sequencing (NGS), which is gradually 
      becoming the gold standard. We performed a retrospective study to compare ALK and 
      ROS1 rearrangement results from immunocytochemistry, FISH and NGS methods from 
      131 cytological samples. Compared to NGS, the sensitivity and specificity of ICC 
      were 0.79 and 0.91, respectively, for ALK, and 1 and 0.87 for ROS1. Regarding 
      FISH, the sensitivity and specificity were both at 1 for ALK and ROS1 probes. 
      False-positive cases obtained by ICC were systematically corrected by FISH. When 
      using ICC and FISH techniques, results are very close to NGS. The false-positive 
      cases obtained by ICC are corrected by FISH, and the true-positive cases are 
      confirmed. NGS has the potential to improve the detection of ALK and ROS1 
      rearrangements in cytological samples; however, the cost of this technique is 
      still much higher than the sequential use of ICC and FISH.
FAU - Frankel, Diane
AU  - Frankel D
AUID- ORCID: 0000-0003-3321-6951
AD  - Aix Marseille Univ, APHM, INSERM, MMG, Hopital la Timone, Service de Biologie 
      Cellulaire, 13005 Marseille, France.
FAU - Nanni, Isabelle
AU  - Nanni I
AD  - APHM, Faculte de Medecine Nord, Service d'Oncobiologie, 13015 Marseille, France.
FAU - Ouafik, L'Houcine
AU  - Ouafik L
AD  - APHM, Faculte de Medecine Nord, Service d'Oncobiologie, 13015 Marseille, France.
AD  - Aix Marseille Univ, CNRS, INP, Inst Neurophysiopathol, 13005 Marseille, France.
FAU - Camilla, Clara
AU  - Camilla C
AD  - APHM, Faculte de Medecine Nord, Service d'Oncobiologie, 13015 Marseille, France.
FAU - Pellegrino, Eric
AU  - Pellegrino E
AUID- ORCID: 0000-0002-2422-5947
AD  - APHM, Faculte de Medecine Nord, Service d'Oncobiologie, 13015 Marseille, France.
FAU - Beaufils, Nathalie
AU  - Beaufils N
AD  - APHM, Faculte de Medecine Nord, Service d'Oncobiologie, 13015 Marseille, France.
FAU - Greillier, Laurent
AU  - Greillier L
AUID- ORCID: 0000-0002-5807-9503
AD  - Multidisciplinary Oncology and Therapeutic Innovations, Marseille University 
      Hospital (APHM), Aix Marseille University, 13015 Marseille, France.
FAU - Dutau, Herve
AU  - Dutau H
AD  - Department of Thoracic Oncology, Pleural Diseases and Interventional Pulmonology, 
      APHM, 13015 Marseille, France.
FAU - Astoul, Philippe
AU  - Astoul P
AUID- ORCID: 0000-0003-3988-1792
AD  - Department of Thoracic Oncology, Pleural Diseases and Interventional Pulmonology, 
      APHM, 13015 Marseille, France.
FAU - Kaspi, Elise
AU  - Kaspi E
AUID- ORCID: 0000-0003-0458-5109
AD  - Aix Marseille Univ, APHM, INSERM, MMG, Hopital la Timone, Service de Biologie 
      Cellulaire, 13005 Marseille, France.
FAU - Roll, Patrice
AU  - Roll P
AUID- ORCID: 0000-0002-0045-5641
AD  - Aix Marseille Univ, APHM, INSERM, MMG, Hopital la Timone, Service de Biologie 
      Cellulaire, 13005 Marseille, France.
LA  - eng
PT  - Journal Article
DEP - 20220912
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (ROS1 protein, human)
SB  - IM
MH  - Anaplastic Lymphoma Kinase/genetics
MH  - *Carcinoma, Non-Small-Cell Lung/diagnosis/genetics
MH  - Gene Rearrangement
MH  - High-Throughput Nucleotide Sequencing/methods
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Lung Neoplasms/diagnosis/genetics
MH  - Protein-Tyrosine Kinases/genetics
MH  - Proto-Oncogene Proteins/genetics
MH  - Retrospective Studies
PMC - PMC9502752
OTO - NOTNLM
OT  - ALK
OT  - FISH
OT  - NGS
OT  - ROS1
OT  - adenocarcinoma
OT  - cytology
OT  - immunocytochemistry
OT  - lung cancer
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/24 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/09/12
CRDT- 2022/09/23 01:24
PHST- 2022/08/02 00:00 [received]
PHST- 2022/08/30 00:00 [revised]
PHST- 2022/09/06 00:00 [accepted]
PHST- 2022/09/23 01:24 [entrez]
PHST- 2022/09/24 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/09/12 00:00 [pmc-release]
AID - ijms231810556 [pii]
AID - ijms-23-10556 [pii]
AID - 10.3390/ijms231810556 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Sep 12;23(18):10556. doi: 10.3390/ijms231810556.