PMID- 36142793
OWN - NLM
STAT- MEDLINE
DCOM- 20220926
LR  - 20220928
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 18
DP  - 2022 Sep 17
TI  - Different Approaches for the Profiling of Cancer Pathway-Related Genes in 
      Glioblastoma Cells.
LID - 10.3390/ijms231810883 [doi]
LID - 10883
AB  - Deregulation of signalling pathways that regulate cell growth, survival, 
      metabolism, and migration can frequently lead to the progression of cancer. Brain 
      tumours are a large group of malignancies characterised by inter- and 
      intratumoral heterogeneity, with glioblastoma (GBM) being the most aggressive and 
      fatal. The present study aimed to characterise the expression of cancer 
      pathway-related genes (n = 84) in glial tumour cell lines (A172, SW1088, and 
      T98G). The transcriptomic data obtained by the qRT-PCR method were compared to 
      different control groups, and the most appropriate control for subsequent 
      interpretation of the obtained results was chosen. We analysed three widely used 
      control groups (non-glioma cells) in glioblastoma research: Human Dermal 
      Fibroblasts (HDFa), Normal Human Astrocytes (NHA), and commercially available 
      mRNAs extracted from healthy human brain tissues (hRNA). The gene expression 
      profiles of individual glioblastoma cell lines may vary due to the selection of a 
      different control group to correlate with. Moreover, we present the original 
      multicriterial decision making (MCDM) for the possible characterization of gene 
      expression profiles. We observed deregulation of 75 genes out of 78 tested in the 
      A172 cell line, while T98G and SW1088 cells exhibited changes in 72 genes. By 
      comparing the delta cycle threshold value of the tumour groups to the mean value 
      of the three controls, only changes in the expression of 26 genes belonging to 
      the following pathways were identified: angiogenesis FGF2; apoptosis APAF1, 
      CFLAR, XIAP; cellular senescence BM1, ETS2, IGFBP5, IGFBP7, SOD1, TBX2; DNA 
      damage and repair ERCC5, PPP1R15A; epithelial to mesenchymal transition SNAI3, 
      SOX10; hypoxia ADM, ARNT, LDHA; metabolism ATP5A1, COX5A, CPT2, PFKL, UQCRFS1; 
      telomeres and telomerase PINX1, TINF2, TNKS, and TNKS2. We identified a human 
      astrocyte cell line and normal human brain tissue as the appropriate control 
      group for an in vitro model, despite the small sample size. A different method of 
      assessing gene expression levels produced the same disparities, highlighting the 
      need for caution when interpreting the accuracy of tumorigenesis markers.
FAU - Majercikova, Zuzana
AU  - Majercikova Z
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, 
      Comenius University in Bratislava, Mala Hora 11161/4D, SK-03601 Martin, Slovakia.
FAU - Dibdiakova, Katarina
AU  - Dibdiakova K
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, 
      Comenius University in Bratislava, Mala Hora 11161/4D, SK-03601 Martin, Slovakia.
FAU - Gala, Michal
AU  - Gala M
AD  - Department of Biophysics, Faculty of Science, University of P.J. Safarik, Jesenna 
      5, SK-04001 Kosice, Slovakia.
FAU - Horvath, Denis
AU  - Horvath D
AUID- ORCID: 0000-0001-9053-1419
AD  - Center for Interdisciplinary Biosciences, Technology and Innovation Park, 
      University of P.J. Safarik, Trieda SNP 1, SK-04011 Kosice, Slovakia.
FAU - Murin, Radovan
AU  - Murin R
AUID- ORCID: 0000-0002-2952-0398
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, 
      Comenius University in Bratislava, Mala Hora 11161/4D, SK-03601 Martin, Slovakia.
FAU - Zoldak, Gabriel
AU  - Zoldak G
AUID- ORCID: 0000-0002-5271-8837
AD  - Center for Interdisciplinary Biosciences, Technology and Innovation Park, 
      University of P.J. Safarik, Trieda SNP 1, SK-04011 Kosice, Slovakia.
FAU - Hatok, Jozef
AU  - Hatok J
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, 
      Comenius University in Bratislava, Mala Hora 11161/4D, SK-03601 Martin, Slovakia.
LA  - eng
GR  - APVV-18-0088/Slovak Research and Development Agency/
GR  - ITMS2014+: 313011V455/Open scientific community for modern interdisciplinary 
      research in medicine (Acronym: OPENMED)/
GR  - vvgs-pf-2022-2116/ERDF and Internal Scientific Grants System of Faculty of 
      Science UPJS/
PT  - Journal Article
DEP - 20220917
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (PINX1 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
RN  - EC 2.4.2.30 (TNKS2 protein, human)
RN  - EC 2.4.2.30 (Tankyrases)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - *Brain Neoplasms/metabolism
MH  - Cell Cycle Proteins/metabolism
MH  - Cell Line, Tumor
MH  - Epithelial-Mesenchymal Transition
MH  - Fibroblast Growth Factor 2/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - *Glioblastoma/metabolism
MH  - Humans
MH  - Superoxide Dismutase-1/genetics
MH  - *Tankyrases/metabolism
MH  - *Telomerase/metabolism
MH  - Tumor Suppressor Proteins/genetics
PMC - PMC9504477
OTO - NOTNLM
OT  - cancer pathway
OT  - glioblastoma
OT  - mRNA
OT  - multicriterial analysis
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/24 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/09/17
CRDT- 2022/09/23 01:26
PHST- 2022/08/05 00:00 [received]
PHST- 2022/09/07 00:00 [revised]
PHST- 2022/09/13 00:00 [accepted]
PHST- 2022/09/23 01:26 [entrez]
PHST- 2022/09/24 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/09/17 00:00 [pmc-release]
AID - ijms231810883 [pii]
AID - ijms-23-10883 [pii]
AID - 10.3390/ijms231810883 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Sep 17;23(18):10883. doi: 10.3390/ijms231810883.