PMID- 36144553
OWN - NLM
STAT- MEDLINE
DCOM- 20220926
LR  - 20220928
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 18
DP  - 2022 Sep 8
TI  - Aptamer Selection Based on Microscale Electrophoretic Filtration Using a 
      Hydrogel-Plugged Capillary Device.
LID - 10.3390/molecules27185818 [doi]
LID - 5818
AB  - This study reports a novel aptamer selection method based on microscale 
      electrophoretic filtration. Aptamers are versatile materials that recognize 
      specific targets and are attractive for their applications in biosensors, 
      diagnosis, and therapy. However, their practical applications remain scarce due 
      to issues with conventional selection methods, such as complicated operations, 
      low-efficiency separation, and expensive apparatus. To overcome these drawbacks, 
      a selection method based on microscale electrophoretic filtration using a 
      capillary partially filled with hydrogel was developed. The electrophoretic 
      filtration of model target proteins (immunoglobulin E (IgE)) using hydrogel, the 
      electrokinetic injection of DNAs to interact with the trapped proteins, the 
      elimination of DNAs with weak interactions, and the selective acquisition of 
      aptamer candidates with strong interactions were successfully demonstrated, 
      revealing the validity of the proposed concept. Two aptamer candidates for IgE 
      were obtained after three selection cycles, and their affinity for the target was 
      confirmed to be less than 1 nM based on their dissociation constant (K(D)) 
      values. Therefore, the proposed method allows for the selection of aptamers with 
      simple operations, highly effective separation based on electrophoresis and 
      filtration, and a relatively cheap apparatus with disposable devices.
FAU - Takao, Junku
AU  - Takao J
AD  - Department of Applied Chemistry, Graduate School of Engineering, Osaka 
      Metropolitan University, Osaka 545-0051, Japan.
FAU - Nagai, Reina
AU  - Nagai R
AD  - Department of Applied Chemistry, Graduate School of Engineering, Osaka 
      Metropolitan University, Osaka 545-0051, Japan.
FAU - Endo, Tatsuro
AU  - Endo T
AUID- ORCID: 0000-0002-5704-5483
AD  - Department of Applied Chemistry, Graduate School of Engineering, Osaka 
      Metropolitan University, Osaka 545-0051, Japan.
FAU - Hisamoto, Hideaki
AU  - Hisamoto H
AUID- ORCID: 0000-0003-1067-4116
AD  - Department of Applied Chemistry, Graduate School of Engineering, Osaka 
      Metropolitan University, Osaka 545-0051, Japan.
FAU - Sueyoshi, Kenji
AU  - Sueyoshi K
AD  - Department of Applied Chemistry, Graduate School of Engineering, Osaka 
      Metropolitan University, Osaka 545-0051, Japan.
AD  - Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science 
      and Technology Agency (JST), Saitama 332-0012, Japan.
LA  - eng
GR  - JPMJPR19H7/Japan Science and Technology Agency/
PT  - Journal Article
DEP - 20220908
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Hydrogels)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Aptamers, Nucleotide/metabolism
MH  - Electrophoresis
MH  - Hydrogels
MH  - Immunoglobulin E
MH  - *SELEX Aptamer Technique/methods
PMC - PMC9505737
OTO - NOTNLM
OT  - aptamer selection
OT  - microscale electrophoretic filtration
OT  - systematic evolution of ligands by exponential enrichment (SELEX)
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/24 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/09/08
CRDT- 2022/09/23 01:36
PHST- 2022/06/20 00:00 [received]
PHST- 2022/08/20 00:00 [revised]
PHST- 2022/09/02 00:00 [accepted]
PHST- 2022/09/23 01:36 [entrez]
PHST- 2022/09/24 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/09/08 00:00 [pmc-release]
AID - molecules27185818 [pii]
AID - molecules-27-05818 [pii]
AID - 10.3390/molecules27185818 [doi]
PST - epublish
SO  - Molecules. 2022 Sep 8;27(18):5818. doi: 10.3390/molecules27185818.