PMID- 36146751
OWN - NLM
STAT- MEDLINE
DCOM- 20220926
LR  - 20221005
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 9
DP  - 2022 Aug 31
TI  - Porcine Reproductive and Respiratory Syndrome virus (PRRSv): A Cross-Sectional 
      Study on ELISA Seronegative, Multivaccinated Sows.
LID - 10.3390/v14091944 [doi]
LID - 1944
AB  - Vaccination against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) 
      is widely used to control clinical disease, but the effectiveness appears in some 
      cases to be suboptimal. Field reports have stated the presence of routinely 
      PRRSv-vaccinated but ELISA seronegative sows: the ELISA non-responders. The real 
      extent of this phenomenon (prevalence-origin-consequences) was not yet 
      investigated. In this study, the prevalence of ELISA non-responders was assessed 
      by measuring PRRSv-specific antibodies in 1400 sows, originating from 70 
      PRRSv-vaccinating sow herds, using IDEXX ELISA (ELISA 1) and CIVTEST E/S ELISA 
      (ELISA 2). Neutralizing antibodies (NAbs) were quantified in a virus 
      neutralization assay. Univariable logistic regression was used to identify herd 
      risk factors for the presence of ELISA non-responders. The global prevalence of 
      non-responders varied from 3.5% (ELISA 1) to 4.1% (ELISA 2), the herd-level 
      prevalence was 40% and the within-herd prevalence ranged from 5% to 20% (ELISA 1) 
      and from 5% to 30% (ELISA 2). The ELISA non-responders had significantly lower 
      NAbs than the ELISA responders. Herds using the combination of one modified live 
      vaccine and one killed vaccine had a significantly reduced risk of having ELISA 
      non-responders. A first assessment of the prevalence and possible consequences of 
      ELISA non-responders has been provided by this study. The clinical importance, 
      origin and underlying immunological mechanisms warrant further research.
FAU - Fiers, Jorian
AU  - Fiers J
AUID- ORCID: 0000-0002-3721-274X
AD  - Unit Viral Re-emerging, Enzootic and Bee diseases, Department Infectious diseases 
      in animals, Sciensano, Groeselenbergstraat 99, 1066 Ukkel, Belgium.
AD  - Unit of Porcine Health Management, Department of Reproduction, Obstetrics and 
      Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 
      9820 Merelbeke, Belgium.
FAU - Tignon, Marylene
AU  - Tignon M
AUID- ORCID: 0000-0001-9781-5289
AD  - Unit Viral Re-emerging, Enzootic and Bee diseases, Department Infectious diseases 
      in animals, Sciensano, Groeselenbergstraat 99, 1066 Ukkel, Belgium.
FAU - Cay, Ann Brigitte
AU  - Cay AB
AD  - Unit Viral Re-emerging, Enzootic and Bee diseases, Department Infectious diseases 
      in animals, Sciensano, Groeselenbergstraat 99, 1066 Ukkel, Belgium.
FAU - Simons, Xavier
AU  - Simons X
AUID- ORCID: 0000-0002-9163-9381
AD  - Unit Veterinary Epidemiology, Department Epidemiology and Public Health, 
      Sciensano, Rue Ernest Blerot 1, 1070 Anderlecht, Belgium.
FAU - Maes, Dominiek
AU  - Maes D
AD  - Unit of Porcine Health Management, Department of Reproduction, Obstetrics and 
      Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 
      9820 Merelbeke, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220831
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing
MH  - Antibodies, Viral
MH  - Cross-Sectional Studies
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - *Porcine Reproductive and Respiratory Syndrome/diagnosis/epidemiology/prevention 
      & control
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - Vaccines, Inactivated
MH  - *Viral Vaccines
PMC - PMC9501492
OTO - NOTNLM
OT  - PRRSv
OT  - immunology
OT  - non-responder
OT  - sow
OT  - vaccination
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/24 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/08/31
CRDT- 2022/09/23 01:49
PHST- 2022/08/02 00:00 [received]
PHST- 2022/08/26 00:00 [revised]
PHST- 2022/08/29 00:00 [accepted]
PHST- 2022/09/23 01:49 [entrez]
PHST- 2022/09/24 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/08/31 00:00 [pmc-release]
AID - v14091944 [pii]
AID - viruses-14-01944 [pii]
AID - 10.3390/v14091944 [doi]
PST - epublish
SO  - Viruses. 2022 Aug 31;14(9):1944. doi: 10.3390/v14091944.