PMID- 36151016
OWN - NLM
STAT- MEDLINE
DCOM- 20221123
LR  - 20230306
IS  - 1879-680X (Electronic)
IS  - 1761-7727 (Linking)
VI  - 20
IP  - 4
DP  - 2022 Dec
TI  - Effects of autologous leukocyte-platelet rich fibrin (L-PRF) on the rate of 
      maxillary canine retraction and various biomarkers in gingival crevicular fluid 
      (GCF): A split mouth randomized controlled trial.
PG  - 100681
LID - S1761-7227(22)00096-1 [pii]
LID - 10.1016/j.ortho.2022.100681 [doi]
AB  - OBJECTIVES: Primary objective: To evaluate the effect of L-PRF on the rate of 
      maxillary canine retraction. SECONDARY OBJECTIVE: To validate the rate of 
      maxillary canine retraction by identifying the levels of Interleukin-1beta (IL-1beta) 
      and Tumour Necrosis Factor-alpha (TNF-alpha) in the Gingival Crevicular Fluid (GCF). 
      MATERIALS AND METHOD: Fifteen females (age range, 18-25 years) with Class I 
      bimaxillary dentoalveolar protrusion malocclusions were included. After levelling 
      and alignment of maxillary arches, 1st premolars were extracted from both sides. 
      Canines were retracted immediately after the extraction of 1st premolars in 
      control sides and placement of L-PRF plugs in the experimental sides. The amount 
      of canine retraction was evaluated from study models recorded before the 
      extraction of 1st premolars (T(0)) and at 1-week (T(1)), 2-weeks (T(2)), 4-weeks 
      (T(3)), and 8-weeks (T(4)) after the beginning of canine retraction by using 
      digital model superimpositions. The concentrations of IL-1beta and TNF-alpha were 
      evaluated from the GCF collected at T(0), T(1), T(2), T(3), and T(4) by using 
      commercially available human IL-1beta and TNF-alpha ELISA kits. RESULTS: The amount of 
      canine retraction in the experimental sides was statistically greater during 
      T(0)-T(1) (P=0.038),T(1)-T(2) (P=0.002), and T(2)-T(3) (P=0.011) periods. Total 
      canine retraction during T(0)-T(4) was statistically greater in experimental 
      sides (2.43+/-0.46mm) than control sides (2.08+/-0.28mm) (P=0.001). The concentration 
      of IL-1beta increased significantly in the experimental sides at T(1) (P=0.000),T(2) 
      (P=0.000), and T(3) (P=0.032). The concentration of TNF-alpha increased both in the 
      control and experimental sides, but it was statistically greater in the 
      experimental sides compared to control sides at T(1) (P=0.000) and T(2) 
      (P=0.006). A positive but not statistically significant correlation was noted 
      between the rate of canine retraction and the concentrations of cytokines such as 
      IL-1beta and TNF-alpha in GCF. CONCLUSION: Over an 8-week period, autologous L-PRF 
      statistically accelerated the rate of maxillary canine retraction, but only by 
      0.35mm. This small acceleration occurred mainly in the first 4 weeks. During the 
      following 4 weeks, the amount of canine retraction was comparable on the 
      experimental and control sides.
CI  - Copyright (c) 2022 CEO. Published by Elsevier Masson SAS. All rights reserved.
FAU - Barhate, Uday H
AU  - Barhate UH
AD  - All India Institute of Medical Sciences, Department of Dentistry, Sijua, 
      Bhubaneswar, Odisha, India.
FAU - Duggal, Isha
AU  - Duggal I
AD  - All India Institute of Medical Sciences, Department of Orthodontics and 
      Dentofacial Deformities, Ansari Nagar, New Delhi, India.
FAU - Mangaraj, Manaswani
AU  - Mangaraj M
AD  - All India Institute of Medical Sciences, Department of Biochemistry, Sijua, 
      Bhubaneswar, Odisha, India.
FAU - Sharan, Jitendra
AU  - Sharan J
AD  - All India Institute of Medical Sciences, Department of Dentistry, Sijua, 
      Bhubaneswar, Odisha, India.
FAU - Duggal, Ritu
AU  - Duggal R
AD  - All India Institute of Medical Sciences, Department of Orthodontics and 
      Dentofacial Deformities, Ansari Nagar, New Delhi, India.
FAU - Jena, Ashok Kumar
AU  - Jena AK
AD  - All India Institute of Medical Sciences, Department of Dentistry, Sijua, 
      Bhubaneswar, Odisha, India. Electronic address: 
      dent_ashok@aiimsbhubaneswar.edu.in.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20220921
PL  - France
TA  - Int Orthod
JT  - International orthodontics
JID - 101184882
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Female
MH  - Humans
MH  - Adolescent
MH  - Young Adult
MH  - Adult
MH  - *Gingival Crevicular Fluid
MH  - *Platelet-Rich Fibrin
MH  - Tooth Movement Techniques
MH  - Tumor Necrosis Factor-alpha
MH  - Mouth
MH  - Biomarkers
MH  - Leukocytes
OTO - NOTNLM
OT  - Accelerated orthodontics
OT  - Canine retraction
OT  - Interleukin-1beta
OT  - L-PRF
OT  - Orthodontic tooth movement
OT  - TNF-alpha
EDAT- 2022/09/24 06:00
MHDA- 2022/11/24 06:00
CRDT- 2022/09/23 22:06
PHST- 2022/05/25 00:00 [received]
PHST- 2022/07/19 00:00 [revised]
PHST- 2022/07/24 00:00 [accepted]
PHST- 2022/09/24 06:00 [pubmed]
PHST- 2022/11/24 06:00 [medline]
PHST- 2022/09/23 22:06 [entrez]
AID - S1761-7227(22)00096-1 [pii]
AID - 10.1016/j.ortho.2022.100681 [doi]
PST - ppublish
SO  - Int Orthod. 2022 Dec;20(4):100681. doi: 10.1016/j.ortho.2022.100681. Epub 2022 
      Sep 21.