PMID- 36154836
OWN - NLM
STAT- MEDLINE
DCOM- 20221006
LR  - 20221018
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 657
DP  - 2022 Nov 15
TI  - Interaction between Benzo[a]anthracene 7,2-dione 7-oxime (BZA) and calf thymus 
      dsDNA using electroanalytical genosensor.
PG  - 114905
LID - S0003-2697(22)00365-7 [pii]
LID - 10.1016/j.ab.2022.114905 [doi]
AB  - In the present study, the objective was to evaluate in situ interaction between 
      Benzo[a]anthracene 7,2-dione 7-oxime (BZA) and calf thymus dsDNA (ct-dsDNA) using 
      electroanalytical genosensor. Analytical techniques based on Ultraviolet/Visible 
      (UV-Vis) spectroscopy and electroanalytical were used to investigate the 
      interaction processes in solution and immobilized on carbon screen-printed 
      electrodes modified with electrochemical mediator Meldola blue. In addition, was 
      possible to evaluate the degree of damage caused to the genetic material by the 
      analyte through of toxicity estimate (S%). The interaction evaluated by 
      genosensor showed processes of intercalation, degradation, and breaks of the 
      double strand of ct-dsDNA, suggesting that the interaction simulates highly toxic 
      (values varying from 0.6 to 0.8 muA in 48 h of interaction), such as 8-oxoguanine 
      (+0.48 V), which is a by-product of guanine oxidation. Furthermore, monitoring A 
      (+1.10 V) after 1 h showed an S% value between 50 and 90%, indicative of high 
      toxicity, and monitoring G (+0.85 V), which showed S>90%, indicated no toxicity 
      after 10 min. Overall, the electroanalytical genosensor developed in a 
      miniaturized system displayed good reproducibility and stability over time being 
      a quick alternative for assesses the degree of toxicity between toxic xenobiotics 
      and biologically electroactive molecules, such as DNA.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Pinheiro-Sousa, Debora Batista
AU  - Pinheiro-Sousa DB
AD  - Coordenacao do Curso de Engenharia Ambiental, Universidade Federal do Maranhao, 
      CEP 65800-000, Balsas, MA, Brazil; Programa de Pos-Graduacao em Biodiversidade e 
      Biotecnologia (REDE BIONORTE), Universidade Estadual do Maranhao, CEP 65055-310 
      Sao Luis, MA, Brazil. Electronic address: debora.batista@ufma.br.
FAU - Sousa Lima, Mayara Ingrid
AU  - Sousa Lima MI
AD  - Departamento de Biologia, Universidade Federal do Maranhao, Campus Paulo VI, CEP 
      65055-970, Sao Luis, MA, Brazil.
FAU - Goncalves, Ricardo Mendes
AU  - Goncalves RM
AD  - Departamento de Biologia, Universidade Federal do Maranhao, Campus Paulo VI, CEP 
      65055-970, Sao Luis, MA, Brazil.
FAU - Silva Santos, Debora Martins
AU  - Silva Santos DM
AD  - Departamento de Biologia, Universidade Estadual do Maranhao, CEP 65080-805 Sao 
      Luis, MA, Brazil.
FAU - Vieira de Carvalho Neta, Alcina
AU  - Vieira de Carvalho Neta A
AD  - Departamento de Biologia, Universidade Estadual do Maranhao, CEP 65080-805 Sao 
      Luis, MA, Brazil.
FAU - Benjamim, Laercio Dos Anjos
AU  - Benjamim LDA
AD  - Departamento de Medicina Veterinaria, Universidade Federal de Vicosa, CEP, 
      36570-000, Vicosa, MG, Brazil.
FAU - Nunes, Gilvanda Silva
AU  - Nunes GS
AD  - Departamento de Tecnologia Quimica, Universidade Federal do Maranhao, CEP 
      65080-805 Sao Luis, MA, Brazil.
FAU - Brasil de Oliveira Marques, Paulo Roberto
AU  - Brasil de Oliveira Marques PR
AD  - Coordenacao do Curso de Licenciaturas em Ciencias Naturais (Biologia), 
      Universidade Federal do Maranhao, CEP 65400-000, Codo, MA, Brazil.
FAU - Fortes Carvalho-Neta, Raimunda Nonata
AU  - Fortes Carvalho-Neta RN
AD  - Departamento de Biologia, Universidade Estadual do Maranhao, CEP 65080-805 Sao 
      Luis, MA, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220922
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Anthracenes)
RN  - 0 (Oximes)
RN  - 5Z93L87A1R (Guanine)
RN  - 7440-44-0 (Carbon)
RN  - 9007-49-2 (DNA)
RN  - EH46A1TLD7 (anthracene)
SB  - IM
MH  - Anthracenes
MH  - *Biosensing Techniques/methods
MH  - Carbon/chemistry
MH  - DNA/chemistry
MH  - Electrodes
MH  - Guanine
MH  - *Oximes
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - 8-Oxoguanine
OT  - Chronoamperometry
OT  - Deoxyribonucleic acid
OT  - Oxidative DNA damage
OT  - Screen-printed electrodes
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/09/27 06:00
MHDA- 2022/10/07 06:00
CRDT- 2022/09/26 15:02
PHST- 2022/02/27 00:00 [received]
PHST- 2022/08/31 00:00 [revised]
PHST- 2022/09/13 00:00 [accepted]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/10/07 06:00 [medline]
PHST- 2022/09/26 15:02 [entrez]
AID - S0003-2697(22)00365-7 [pii]
AID - 10.1016/j.ab.2022.114905 [doi]
PST - ppublish
SO  - Anal Biochem. 2022 Nov 15;657:114905. doi: 10.1016/j.ab.2022.114905. Epub 2022 
      Sep 22.