PMID- 36156628
OWN - NLM
STAT- MEDLINE
DCOM- 20220928
LR  - 20221001
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 40
IP  - 9
DP  - 2022 Sep
TI  - [Rapid determination of 22 functional components in cosmetics using ultra-high 
      performance liquid chromatography-linear ion trap/orbitrap high resolution mass 
      spectrometry].
PG  - 817-824
LID - 10.3724/SP.J.1123.2022.03037 [doi]
AB  - Plant components from extracts of Sophora flavescens, rhodiola, ginseng, Centella 
      asiatica, and tea play important roles in skin whitening, moisturizing, 
      anti-aging, sun protection, anti-inflammation, antiseptic, bacteriostatic, and 
      other effects of cosmetics. At present, no relevant standard methods have been 
      established to detect the addition amounts of plant extracts in cosmetics. In 
      addition, plant extracts listed in product labels may be undetectable due to 
      their addition in trace quantities and the lack of technical support. Therefore, 
      a quantitative method for the simultaneous determination of 22 functional 
      components in cosmetics was established by ultra-high performance liquid 
      chromatography-linear ion trap/orbitrap high resolution mass spectrometry 
      (UHPLC-LTQ/Orbitrap MS). Target compounds were extracted with methanol from 
      samples using ultrasonic extraction, and then separated on a C18 column (100 mm x 
      2.1 mm, 1.8 mum) with gradient elution of 0.1% (v/v) formic acid aqueous solution 
      (A) and acetonitrile (B). The gradient elution program were as follows: 0-5 min, 
      5%B-8%B; 5-25 min, 8%B-60%B; 25-35 min, 60%B-80%B; 35-36 min, 80%B-5%B; 36-45 
      min, 5%B. The flow rate was 0.3 mL/min and the injection volume was 5 muL. 
      Accurate masses of precursor ions were used to detect cosmetic functional 
      components in positive ionization mode. The fragment ions obtained by higher 
      energy collisional dissociation were used for confirmation of the functional 
      components. Each compound showed good linearity. The limits of detection (LODs) 
      were in the range of 0.003-2.01 mg/kg, and the limits of quantification (LOQs) 
      were in the range of 0.02-4.36 mg/kg. Recoveries at three levels were 
      63.2%-125.1%, and relative standard deviations (RSDs) were 0.18%-10.9%. 
      Fifty-four batches of samples labeled with four monomer functional components and 
      nine plant extracts were tested. In the 17 batches of samples labeled with 
      nicotinamide, 4 batches labeled with caffeine, and 6 batches labeled with Sophora 
      flavescens root extract, the labeled functional components were detected. One out 
      of 11 batches of samples labeled with D-panthenol was not detected. Three of the 
      seven batches of samples labeled with ascorbyl glucoside were not detected. In 
      the 21 batches of samples labeled with licorice extracts, the corresponding 
      functional components were not detected in 9 batches. In the 21 batches of 
      samples labeled with Centella asiatica extract, the corresponding functional 
      components were not detected in 11 batches. In the 13 batches of samples labeled 
      with tea extract, the corresponding functional components were not detected in 8 
      batches. In 11 of the 12 batches containing ginseng root extract, the 
      corresponding functional components were not detected. In five of the six batches 
      of astragalus membranaceus root extract samples, the corresponding functional 
      components were not detected. In samples labeled with Polygonum cuspidatum root 
      extract, Rehmannia glutinosa root extract, and Ophiopogon japonicus root extract, 
      the corresponding functional components were detected. The method is simple, 
      rapid, reliable, accurate, and suitable for the determination of the 22 
      functional components in cosmetics.
FAU - Xiong, Chensihui
AU  - Xiong C
AD  - School of Pharmaceutical Science, Hubei University of Chinese Medicine, Wuhan 
      430065, China.
AD  - Hubei Institute for Drug Control, Wuhan 430075, China.
FAU - Ding, Tianming
AU  - Ding T
AD  - Hubei Institute for Drug Control, Wuhan 430075, China.
FAU - Liu, Jie
AU  - Liu J
AD  - Hubei Institute for Drug Control, Wuhan 430075, China.
FAU - Yi, Ou
AU  - Yi O
AD  - Wuhan Beauty Evolution Information Technology Co., Ltd., Wuhan 437000, China.
FAU - Ding, Xiaoping
AU  - Ding X
AD  - Hubei Institute for Drug Control, Wuhan 430075, China.
FAU - Xie, Yun
AU  - Xie Y
AD  - School of Pharmaceutical Science, Hubei University of Chinese Medicine, Wuhan 
      430065, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Acetonitriles)
RN  - 0 (Anti-Infective Agents, Local)
RN  - 0 (Cosmetics)
RN  - 0 (Glucosides)
RN  - 0 (Ions)
RN  - 0 (Plant Extracts)
RN  - 0 (Tea)
RN  - 25X51I8RD4 (Niacinamide)
RN  - 3G6A5W338E (Caffeine)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Acetonitriles/analysis
MH  - *Anti-Infective Agents, Local/analysis
MH  - Caffeine/analysis
MH  - Chromatography, High Pressure Liquid
MH  - *Cosmetics/analysis
MH  - Glucosides
MH  - Ions
MH  - Mass Spectrometry
MH  - Methanol/analysis
MH  - Niacinamide/analysis
MH  - Plant Extracts
MH  - Tea
PMC - PMC9520369
OTO - NOTNLM
OT  - cosmetics
OT  - functional components
OT  - linear ion trap/orbitrap high resolution mass spectrometry (LTQ/Orbitrap MS)
OT  - ultra-high performance liquid chromatography (UHPLC)
EDAT- 2022/09/27 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/09/08
CRDT- 2022/09/26 16:45
PHST- 2022/09/26 16:45 [entrez]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/09/08 00:00 [pmc-release]
AID - 10.3724/SP.J.1123.2022.03037 [doi]
PST - ppublish
SO  - Se Pu. 2022 Sep;40(9):817-824. doi: 10.3724/SP.J.1123.2022.03037.