PMID- 36156630
OWN - NLM
STAT- MEDLINE
DCOM- 20220928
LR  - 20221001
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 40
IP  - 9
DP  - 2022 Sep
TI  - [Determination of gypenoside XLVI and LVI in Gynostemma pentaphyllum from Fujian 
      by ultra-high performance liquid chromatography-charged aerosol detector].
PG  - 833-842
LID - 10.3724/SP.J.1123.2022.01024 [doi]
AB  - Gynostemma pentaphyllum (Thunb.) Makino contains dammarane-type triterpenoid 
      saponins, similar to ginseng, with a host of pharmacological activities. However, 
      its planting resources and chemical composition are quite complex. The chemical 
      constituents of Gynostemma pentaphyllum vary drastically among different origins 
      and varieties. Thus, the corresponding quality control methods also need to be 
      different. Currently, limited information is available about the quality control 
      of Gynostemma pentaphyllum from Fujian. A new method based on ultra-high 
      performance liquid chromatography-charged aerosol detection (UHPLC-CAD) was 
      established for the determination of gypenoside XLVI and LVI in Gynostemma 
      pentaphyllum. The major components of Gynostemma pentaphyllum were characterized 
      using UHPLC-quadrupole time-of-flight-mass spectrometry (UHPLC-Q-TOF/MS) combined 
      with UHPLC-CAD. The results revealed gypenoside XLVI, LVI, and their 
      corresponding malonyl-containing acidic saponins as the main components. However, 
      malonylgypenoside XLVI and LVI can easily remove their malonyl group and convert 
      to gypenoside XLVI and LVI during the application of Gynostemma pentaphyllum. In 
      this study, the samples were pretreated using alkali hydrolysis to transform the 
      acid saponins completely, and the final contents of gypenoside XLVI and LVI were 
      determined via UHPLC-CAD. The optimal alkaline hydrolysis, extraction, and liquid 
      chromatography conditions were established. First, the alkaline hydrolysis 
      conditions were optimized. The effects of the volume of ammonia and reaction time 
      on the contents of gypenoside XLVI, LVI, malonylgypenoside XLVI, and LVI were 
      examined. Malonylgypenoside XLVI and LVI could be transformed completely to 
      gypenoside XLVI and LVI by standing for 24 h in an ethanol-water-ammonia 
      (50ratio46ratio4, v/v/v) mixture. Furthermore, the extraction conditions were optimized. 
      Next, effects of the different solvents, extraction time, and solid-liquid ratio 
      on the extraction rates of gypenoside XLVI and LVI were investigated. The 
      extraction method for Gynostemma pentaphyllum powder using the 
      ethanol-water-ammonia (50ratio46ratio4, v/v/v) and a solid-liquid ratio of 1ratio150 (gratiomL) 
      for 30 min was established. Finally, a prepared test solution was separated on a 
      Waters ACQUITY UPLC BEH C18 chromatographic column (100 mmx2.1 mm, 1.7 mum). 
      Acetonitrile and 0.1% (v/v) formic acid aqueous solution were used as the mobile 
      phases for gradient elution. The flow rate was set to 0.5 mL/min and column 
      temperature was maintained at 40 ℃. The separation was detected using a charged 
      aerosol detector. Results indicated that the logarithm of the mass concentrations 
      of gypenoside XLVI and LVI had a linear relationship with the logarithm of the 
      peak area in the range of 9.94-318.00 mug/mL and 12.78-409.00 mug/mL, respectively. 
      The correlation coefficients (r) were 0.9993 and 0.9995, respectively. The limit 
      of detection (LOD) and the limit of quantification (LOQ) of gypenoside XLVI were 
      1.58 mug/mL and 6.36 mug/mL, respectively. The LOD and LOQ of gypenoside LVI were 
      2.05 mug/mL and 8.18 mug/mL, respectively. The relative standard deviations (RSDs) 
      of precision, repeatability, and 24 h stability were less than 2.0% (n=6). The 
      spiked recoveries of gypenoside XLVI were 100.2%-107.2% and the RSD value was 
      2.4%. The spiked recoveries of gypenoside LVI were 97.9%-104.2% and the RSD value 
      was 2.6%. The results of 16 batches of Gynostemma pentaphyllum samples indicated 
      that the gypenoside XLVI content was 0.57%-2.57%, and gypenoside LVI content was 
      0.66%-2.99%. Hence, this method has high sensitivity and good reproducibility. 
      Therefore, it can be used for quality research and quality control of Gynostemma 
      pentaphyllum from Fujian.
FAU - Lu, Pengxin
AU  - Lu P
AD  - School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, 
      Guangzhou 510060, China.
AD  - Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100850, China.
FAU - Li, Gang
AU  - Li G
AD  - School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, 
      Guangzhou 510060, China.
AD  - Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100850, China.
FAU - Zheng, Wei
AU  - Zheng W
AD  - Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100850, China.
FAU - Liang, Haizhen
AU  - Liang H
AD  - Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100850, China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100850, China.
FAU - Chai, Ruiping
AU  - Chai R
AD  - Thermo Fisher Scientific (China) Co., Ltd., Shanghai 201206, China.
FAU - Luo, Dingqiang
AU  - Luo D
AD  - Shaanxi Institute for Food and Drug Control, Xi'an 710065, China.
FAU - Jin, Yan
AU  - Jin Y
AD  - Thermo Fisher Scientific (China) Co., Ltd., Shanghai 201206, China.
FAU - Guo, Baolin
AU  - Guo B
AD  - Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and 
      Peking Union Medical College, Beijing 100193, China.
FAU - Ma, Baiping
AU  - Ma B
AD  - School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, 
      Guangzhou 510060, China.
AD  - Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of 
      Military Sciences, Beijing 100850, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Acetonitriles)
RN  - 0 (Aerosols)
RN  - 0 (Alkalies)
RN  - 0 (Powders)
RN  - 0 (Saponins)
RN  - 0 (Solvents)
RN  - 0 (Triterpenes)
RN  - 0 (gypenoside XLVI)
RN  - 059QF0KO0R (Water)
RN  - 3K9958V90M (Ethanol)
RN  - 7664-41-7 (Ammonia)
SB  - IM
MH  - Acetonitriles
MH  - Aerosols
MH  - Alkalies
MH  - Ammonia
MH  - Chromatography, High Pressure Liquid
MH  - Ethanol
MH  - Gynostemma/chemistry
MH  - Powders
MH  - Reproducibility of Results
MH  - *Saponins/chemistry
MH  - Solvents
MH  - *Triterpenes
MH  - Water
PMC - PMC9520372
OTO - NOTNLM
OT  - Gynostemma pentaphyllum (Thunb.) Makino from Fujian
OT  - gypenoside LVI
OT  - gypenoside XLVI
OT  - ultra-high performance liquid chromatography-charged aerosol detector (UHPLC-CAD)
EDAT- 2022/09/27 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/09/08
CRDT- 2022/09/26 16:45
PHST- 2022/09/26 16:45 [entrez]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/09/08 00:00 [pmc-release]
AID - 10.3724/SP.J.1123.2022.01024 [doi]
PST - ppublish
SO  - Se Pu. 2022 Sep;40(9):833-842. doi: 10.3724/SP.J.1123.2022.01024.