PMID- 36158210
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220928
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 10
DP  - 2022
TI  - Craniofacial tendon development-Characterization of extracellular matrix 
      morphology and spatiotemporal protein distribution.
PG  - 944126
LID - 10.3389/fcell.2022.944126 [doi]
LID - 944126
AB  - Craniofacial (CF) tendons are often affected by traumatic injuries and painful 
      disorders that can severely compromise critical jaw functions, such as 
      mastication and talking. Unfortunately, tendons lack the ability to regenerate, 
      and there are no solutions to restore their native properties or function. An 
      understanding of jaw tendon development could inform tendon regeneration 
      strategies to restore jaw function, however CF tendon development has been 
      relatively unexplored. Using the chick embryo, we identified the jaw-closing 
      Tendon of the musculus Adductor Mandibulae Externus (TmAM) and the jaw-opening 
      Tendon of the musculus Depressor Mandibulae (TmDM) that have similar functions to 
      the masticatory tendons in humans. Using histological and immunohistochemical 
      (IHC) analyses, we characterized the TmAM and TmDM on the basis of cell and 
      extracellular matrix (ECM) morphology and spatiotemporal protein distribution 
      from early to late embryonic development. The TmAM and TmDM were detectable as 
      early as embryonic day (d) 9 based on histological staining and tenascin-C (TNC) 
      protein distribution. Collagen content increased and became more organized, cell 
      density decreased, and cell nuclei elongated over time during development in both 
      the TmAM and TmDM. The TmAM and TmDM exhibited similar spatiotemporal patterns 
      for collagen type III (COL3), but differential spatiotemporal patterns for TNC, 
      lysyl oxidase (LOX), and matrix metalloproteinases (MMPs). Our results 
      demonstrate markers that play a role in limb tendon formation are also present in 
      jaw tendons during embryonic development, implicate COL3, TNC, LOX, MMP2, and 
      MMP9 in jaw tendon development, and suggest TmAM and TmDM possess different 
      developmental programs. Taken together, our study suggests the chick embryo may 
      be used as a model with which to study CF tendon extracellular matrix 
      development, the results of which could ultimately inform therapeutic approaches 
      for CF tendon injuries and disorders.
CI  - Copyright (c) 2022 Korntner, Jana, Kinnard, Leo, Beane, Li, Sengupta, Becker and 
      Kuo.
FAU - Korntner, Stefanie H
AU  - Korntner SH
AD  - Fischell Department of Bioengineering, University of Maryland, College Park, MD, 
      United States.
FAU - Jana, Aniket
AU  - Jana A
AD  - Fischell Department of Bioengineering, University of Maryland, College Park, MD, 
      United States.
FAU - Kinnard, Elizabeth
AU  - Kinnard E
AD  - Fischell Department of Bioengineering, University of Maryland, College Park, MD, 
      United States.
FAU - Leo, Emily
AU  - Leo E
AD  - Fischell Department of Bioengineering, University of Maryland, College Park, MD, 
      United States.
FAU - Beane, Timothy
AU  - Beane T
AD  - Department of Biomedical Engineering, University of Rochester, Rochester, NY, 
      United States.
FAU - Li, Xianmu
AU  - Li X
AD  - Department of Biomedical Engineering, University of Rochester, Rochester, NY, 
      United States.
FAU - Sengupta, Rohit
AU  - Sengupta R
AD  - Fischell Department of Bioengineering, University of Maryland, College Park, MD, 
      United States.
FAU - Becker, Lauren
AU  - Becker L
AD  - Fischell Department of Bioengineering, University of Maryland, College Park, MD, 
      United States.
FAU - Kuo, Catherine K
AU  - Kuo CK
AD  - Fischell Department of Bioengineering, University of Maryland, College Park, MD, 
      United States.
AD  - Department of Biomedical Engineering, University of Rochester, Rochester, NY, 
      United States.
AD  - Center for Musculoskeletal Research, University of Rochester Medical Center, 
      Rochester, NY, United States.
AD  - Department of Orthopaedics, University of Maryland Medical Center, Baltimore, MD, 
      United States.
LA  - eng
PT  - Journal Article
DEP - 20220907
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC9490420
OTO - NOTNLM
OT  - chick embryo
OT  - collagen
OT  - craniofacial
OT  - jaw
OT  - lysyl oxidase
OT  - matrix metalloproteinase
OT  - tendon
OT  - tendon development
EDAT- 2022/09/27 06:00
MHDA- 2022/09/27 06:01
PMCR- 2022/01/01
CRDT- 2022/09/26 17:17
PHST- 2022/05/14 00:00 [received]
PHST- 2022/08/10 00:00 [accepted]
PHST- 2022/09/26 17:17 [entrez]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/09/27 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 944126 [pii]
AID - 10.3389/fcell.2022.944126 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2022 Sep 7;10:944126. doi: 10.3389/fcell.2022.944126. 
      eCollection 2022.