PMID- 36158754
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220928
IS  - 2666-4984 (Electronic)
IS  - 2096-9643 (Print)
IS  - 2666-4984 (Linking)
VI  - 11
DP  - 2022 Jul
TI  - In situ identification of environmental microorganisms with Raman spectroscopy.
PG  - 100187
LID - 10.1016/j.ese.2022.100187 [doi]
LID - 100187
AB  - Microorganisms in natural environments are crucial in maintaining the material 
      and energy cycle and the ecological balance of the environment. However, it is 
      challenging to delineate environmental microbes' actual metabolic pathways and 
      intraspecific heterogeneity because most microorganisms cannot be cultivated. 
      Raman spectroscopy is a culture-independent technique that can collect molecular 
      vibration profiles from cells. It can reveal the physiological and biochemical 
      information at the single-cell level rapidly and non-destructively in situ. The 
      first part of this review introduces the principles, advantages, progress, and 
      analytical methods of Raman spectroscopy applied in environmental microbiology. 
      The second part summarizes the applications of Raman spectroscopy combined with 
      stable isotope probing (SIP), fluorescence in situ hybridization (FISH), 
      Raman-activated cell sorting and genomic sequencing, and machine learning in 
      microbiological studies. Finally, this review discusses expectations of Raman 
      spectroscopy and future advances to be made in identifying microorganisms, 
      especially for uncultured microorganisms.
CI  - (c) 2022 The Authors.
FAU - Cui, Dongyu
AU  - Cui D
AD  - Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), 
      Guangzhou, 511458, China.
AD  - Department of Ocean Science and Engineering, Southern University of Science and 
      Technology, Shenzhen, 518055, China.
FAU - Kong, Lingchao
AU  - Kong L
AD  - State Environmental Protection Key Laboratory of Integrated Surface 
      Water-Groundwater Pollution Control, School of Environmental Science & 
      Engineering, Southern University of Science and Technology, Shenzhen, 518055, 
      China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), 
      Guangzhou, 511458, China.
AD  - Department of Ocean Science and Engineering, Southern University of Science and 
      Technology, Shenzhen, 518055, China.
FAU - Zhu, Yuanqing
AU  - Zhu Y
AD  - Department of Ocean Science and Engineering, Southern University of Science and 
      Technology, Shenzhen, 518055, China.
AD  - Shanghai Sheshan National Geophysical Observatory, Shanghai Earthquake Agency, 
      Shanghai, 200062, China.
FAU - Zhang, Chuanlun
AU  - Zhang C
AD  - Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), 
      Guangzhou, 511458, China.
AD  - Department of Ocean Science and Engineering, Southern University of Science and 
      Technology, Shenzhen, 518055, China.
AD  - Shenzhen Key Laboratory of Marine Archaea Geo-Omics, University of Southern 
      University of Science and Technology, Shenzhen, 518055, China.
AD  - Shanghai Sheshan National Geophysical Observatory, Shanghai Earthquake Agency, 
      Shanghai, 200062, China.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20220521
PL  - Netherlands
TA  - Environ Sci Ecotechnol
JT  - Environmental science and ecotechnology
JID - 9918453988906676
PMC - PMC9488013
OTO - NOTNLM
OT  - Environmental microorganisms
OT  - Metabolic activities
OT  - Raman spectroscopy
OT  - Single cells
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/09/27 06:00
MHDA- 2022/09/27 06:01
PMCR- 2022/05/21
CRDT- 2022/09/26 17:25
PHST- 2022/01/07 00:00 [received]
PHST- 2022/05/13 00:00 [revised]
PHST- 2022/05/15 00:00 [accepted]
PHST- 2022/09/26 17:25 [entrez]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/09/27 06:01 [medline]
PHST- 2022/05/21 00:00 [pmc-release]
AID - S2666-4984(22)00043-6 [pii]
AID - 100187 [pii]
AID - 10.1016/j.ese.2022.100187 [doi]
PST - epublish
SO  - Environ Sci Ecotechnol. 2022 May 21;11:100187. doi: 10.1016/j.ese.2022.100187. 
      eCollection 2022 Jul.