PMID- 36160904
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220928
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Print)
IS  - 1792-0981 (Linking)
VI  - 24
IP  - 4
DP  - 2022 Oct
TI  - LncRNA Meg3 promotes oxygen and glucose deprivation injury by decreasing 
      angiogenesis in hBMECs by targeting the miR‑122‑5p/NDRG3 axis.
PG  - 622
LID - 10.3892/etm.2022.11559 [doi]
LID - 622
AB  - Oxygen-glucose deprivation (OGD) is widely used as an in vitro model for stroke. 
      The present study aimed to explore the mechanisms of action of long non-coding 
      RNA (lncRNA) maternally expressed gene 3 (Meg3) in angiogenesis following OGD. 
      The human brain microvascular endothelial cell line, hCMEC/D3, was used to 
      establish the OGD model. lncRNA Meg3 was highly expressed in hCMEC/D3 cells 
      subjected to OGD. Furthermore, it was found that the overexpression of lncRNA 
      Meg3 decreased the proliferation, migration and angiogenesis of hCMEC/D3 cells 
      subjected to OGD, and increased cell apoptosis. Meg3 silencing exerted the 
      opposite effects. Subsequently, lncRNA Meg3 increased the expression of NDRG 
      family member 3 (NDRG3) by directly binding to miR-122-5p. The overexpression of 
      miR-122-5p and the knockdown of NDRG3 reversed the inhibitory effects of Meg3 
      overexpression on the proliferation, migration and angiogenesis of hCMEC/D3 cells 
      subjected to OGD, as well as the promoting effects of Meg3 overexpression on cell 
      apoptosis. The present study demonstrated that lncRNA Meg3 functions as a 
      competing endogenous RNA by targeting the miR-122-5p/NDRG3 axis in regulating OGD 
      injury.
CI  - Copyright: (c) Luo et al.
FAU - Luo, Zhaoliang
AU  - Luo Z
AD  - Research and Innovation Center, Basic Medical College, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
AD  - Department of Brain Diseases, Chongqing Traditional Chinese Medicine Hospital, 
      Chongqing 400000, P.R. China.
FAU - Gong, Tingliang
AU  - Gong T
AD  - Department of Brain Diseases, Chongqing Traditional Chinese Medicine Hospital, 
      Chongqing 400000, P.R. China.
FAU - Li, Weihong
AU  - Li W
AD  - Research and Innovation Center, Basic Medical College, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Tao, Wenqiang
AU  - Tao W
AD  - Department of Brain Diseases, Chongqing Traditional Chinese Medicine Hospital, 
      Chongqing 400000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20220811
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC9468836
OTO - NOTNLM
OT  - NDRG family member 3
OT  - angiogenesis
OT  - ischemic stroke
OT  - long non-coding RNA maternally expressed gene 3
OT  - microRNA-122-5p
COIS- The authors declare that they have no competing interests.
EDAT- 2022/09/27 06:00
MHDA- 2022/09/27 06:01
PMCR- 2022/08/11
CRDT- 2022/09/26 17:57
PHST- 2022/03/13 00:00 [received]
PHST- 2022/07/22 00:00 [accepted]
PHST- 2022/09/26 17:57 [entrez]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/09/27 06:01 [medline]
PHST- 2022/08/11 00:00 [pmc-release]
AID - ETM-24-4-11559 [pii]
AID - 10.3892/etm.2022.11559 [doi]
PST - epublish
SO  - Exp Ther Med. 2022 Aug 11;24(4):622. doi: 10.3892/etm.2022.11559. eCollection 
      2022 Oct.