PMID- 36162147
OWN - NLM
STAT- MEDLINE
DCOM- 20221017
LR  - 20221017
IS  - 1618-0623 (Electronic)
IS  - 0944-5013 (Linking)
VI  - 265
DP  - 2022 Dec
TI  - Whole cell evaluation and the enzymatic kinetic study of urease from ureolytic 
      bacteria affected by potentially toxic elements.
PG  - 127208
LID - S0944-5013(22)00248-8 [pii]
LID - 10.1016/j.micres.2022.127208 [doi]
AB  - Microbially induced carbonate precipitation (MICP) is a biomineralization process 
      that has various applications in environmental pollution remediation and 
      restoration of a range of building materials. In this study, a ureolytic 
      bacterium, Lysinibacillus sp. GY3, isolated from an E-waste site, was found as a 
      promising catalyst for remediation of heavy metals via the MICP process. This 
      bacterial isolate produced significant amounts of urease and showed a great 
      persistence in immobilization of potentially toxic elements. A reference 
      ureolytic strain, Bacillus megaterium VS1, was selected in order to compare the 
      efficiency of Lysinibacillus sp. GY3. Study on urease localization indicated 80 % 
      more urease activity secreted extracellularly as for Lysinibacillus sp. GY3 
      compared to B. megaterium VS1. From the investigation on effects of metals on 
      both intra- and extra-cellular urease, it was clear that Lysinibacillus sp. GY3 
      produced the most stable urease under conditions of metal pressure, especially 
      retaining more than 70 % activity in the presence of 1 g/L Pb(2+) and Zn(2+). 
      These results suggest that this isolated microorganism could be promisingly 
      introduced in the MICP process to stabilize complex heavy metal pollutions, with 
      reference to the regulating ability under harsh conditions to stabilize urease 
      activity. This species is so important both for its biological features and 
      environmental impacts. In addition, the present study will bring new insight in 
      the field of metal remediation coupled with enzyme engineered biotechnology.
CI  - Copyright (c) 2022 Elsevier GmbH. All rights reserved.
FAU - Li, Weila
AU  - Li W
AD  - Department of Biotechnology and Food Engineering, Technion - Israel Institute of 
      Technology, Haifa 3200003, Israel; Environmental Science and Engineering Program, 
      Guangdong Technion - Israel Institute of Technology, Shantou 515063, China.
FAU - Fishman, Ayelet
AU  - Fishman A
AD  - Department of Biotechnology and Food Engineering, Technion - Israel Institute of 
      Technology, Haifa 3200003, Israel.
FAU - Achal, Varenyam
AU  - Achal V
AD  - Environmental Science and Engineering Program, Guangdong Technion - Israel 
      Institute of Technology, Shantou 515063, China; Technion - Israel Institute of 
      Technology, Haifa 3200003, Israel. Electronic address: 
      varenyam.achal@gtiit.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220921
PL  - Germany
TA  - Microbiol Res
JT  - Microbiological research
JID - 9437794
RN  - 0 (Carbonates)
RN  - 0 (Metals, Heavy)
RN  - 2P299V784P (Lead)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - *Bacillaceae/genetics
MH  - *Bacillus megaterium
MH  - Calcium Carbonate
MH  - Carbonates
MH  - Lead
MH  - *Metals, Heavy
MH  - Urease
OTO - NOTNLM
OT  - Protein structure
OT  - Toxic elements
OT  - Urease
OT  - Ureolytic bacteria
OT  - Whole cell biocatalysis
COIS- Conflict of interest The authors declare no competing interests.
EDAT- 2022/09/27 06:00
MHDA- 2022/10/18 06:00
CRDT- 2022/09/26 18:47
PHST- 2022/05/10 00:00 [received]
PHST- 2022/09/05 00:00 [revised]
PHST- 2022/09/19 00:00 [accepted]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/10/18 06:00 [medline]
PHST- 2022/09/26 18:47 [entrez]
AID - S0944-5013(22)00248-8 [pii]
AID - 10.1016/j.micres.2022.127208 [doi]
PST - ppublish
SO  - Microbiol Res. 2022 Dec;265:127208. doi: 10.1016/j.micres.2022.127208. Epub 2022 
      Sep 21.