PMID- 36162992
OWN - NLM
STAT- MEDLINE
DCOM- 20231120
LR  - 20231120
IS  - 1471-2407 (Electronic)
IS  - 1471-2407 (Linking)
VI  - 22
IP  - 1
DP  - 2022 Sep 26
TI  - LncRNA RPL34-AS1 suppresses the proliferation, migration and invasion of 
      esophageal squamous cell carcinoma via targeting miR-575/ACAA2 axis.
PG  - 1017
LID - 10.1186/s12885-022-10104-6 [doi]
LID - 1017
AB  - BACKGROUND: Long noncoding RNAs (lncRNAs) are abnormally expressed in a broad 
      type of cancers and play significant roles that regulate tumor development and 
      metastasis. However, the pathological roles of lncRNAs in esophageal squamous 
      cell carcinoma (ESCC) remain largely unknown. Here we aimed to investigate the 
      role and regulatory mechanism of the novel lncRNA RPL34-AS1 in the development 
      and progression of ESCC. METHODS: The expression level of RPL34-AS1 in ESCC 
      tissues and cell lines was determined by RT-qPCR. Functional experiments in vitro 
      and in vivo were employed to explore the effects of RPL34-AS1 on tumor growth in 
      ESCC cells. Mechanistically, fluorescence in situ hybridization (FISH), 
      bioinformatics analyses, luciferase reporter assay, RNA immunoprecipitation (RIP) 
      assay and western blot assays were used to detect the regulatory relationship 
      between RPL34-AS1, miR-575 and ACAA2. RESULTS: RPL34-AS1 was significantly 
      down-regulated in ESCC tissues and cells, which was negatively correlated with 
      overall survival in ESCC patients. Functionally, upregulation of RPL34-AS1 
      dramatically suppressed ESCC cell proliferation, colony formation, invasion and 
      migration in vitro, whereas knockdown of RPL34-AS1 elicited the opposite 
      function. Consistently, overexpression of RPL34-AS1 inhibited tumor growth in 
      vivo. Mechanistically, RPL34-AS1 acted as a competing endogenous RNA (ceRNA) of 
      miR-575 to relieve the repressive effect of miR-575 on its target ACAA2, then 
      suppressed the tumorigenesis of ESCC. CONCLUSIONS: Our results reveal a role for 
      RPL34-AS1 in ESCC tumorigenesis and may provide a strategy for using RPL34-AS1 as 
      a potential biomarker and an effect target for patients with ESCC.
CI  - (c) 2022. The Author(s).
FAU - Zhang, Hu
AU  - Zhang H
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, 87 Dingjiaqiao Street, Nanjing, 
      210009, People's Republic of China.
FAU - Pan, Enchun
AU  - Pan E
AD  - Huaian Center for Disease Control and Prevention, Huaian, 223001, People's 
      Republic of China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, 87 Dingjiaqiao Street, Nanjing, 
      210009, People's Republic of China.
FAU - Zhao, Chao
AU  - Zhao C
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, 87 Dingjiaqiao Street, Nanjing, 
      210009, People's Republic of China.
FAU - Liu, Qiwei
AU  - Liu Q
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, 87 Dingjiaqiao Street, Nanjing, 
      210009, People's Republic of China.
FAU - Pu, Yuepu
AU  - Pu Y
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, 87 Dingjiaqiao Street, Nanjing, 
      210009, People's Republic of China.
FAU - Yin, Lihong
AU  - Yin L
AD  - Key Laboratory of Environmental Medicine Engineering, Ministry of Education, 
      School of Public Health, Southeast University, 87 Dingjiaqiao Street, Nanjing, 
      210009, People's Republic of China. lhyin@seu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220926
PL  - England
TA  - BMC Cancer
JT  - BMC cancer
JID - 100967800
RN  - EC 2.3.1.16 (Acetyl-CoA C-Acyltransferase)
RN  - 0 (Biomarkers)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN575 microRNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.3.1.16 (ACAA2 protein, human)
SB  - IM
MH  - Humans
MH  - Acetyl-CoA C-Acyltransferase
MH  - Biomarkers
MH  - Carcinogenesis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - *Esophageal Neoplasms/pathology
MH  - *Esophageal Squamous Cell Carcinoma/pathology
MH  - Gene Expression Regulation, Neoplastic
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics/metabolism
MH  - Neoplastic Processes
MH  - *RNA, Long Noncoding/genetics/metabolism
PMC - PMC9511711
OTO - NOTNLM
OT  - ACAA2
OT  - Esophageal squamous cell carcinoma
OT  - lncRNA RPL34-AS1
OT  - miR-575
COIS- The authors declare no competing financial interests.
EDAT- 2022/09/27 06:00
MHDA- 2022/09/28 06:00
PMCR- 2022/09/26
CRDT- 2022/09/26 23:33
PHST- 2022/04/20 00:00 [received]
PHST- 2022/09/19 00:00 [accepted]
PHST- 2022/09/26 23:33 [entrez]
PHST- 2022/09/27 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/09/26 00:00 [pmc-release]
AID - 10.1186/s12885-022-10104-6 [pii]
AID - 10104 [pii]
AID - 10.1186/s12885-022-10104-6 [doi]
PST - epublish
SO  - BMC Cancer. 2022 Sep 26;22(1):1017. doi: 10.1186/s12885-022-10104-6.