PMID- 36164527
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220928
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 8
IP  - 9
DP  - 2022 Sep
TI  - Molecular classification of soft tissue sarcomas for adequate diagnosis: A study 
      on the northeast population of Morocco.
PG  - e10673
LID - 10.1016/j.heliyon.2022.e10673 [doi]
LID - e10673
AB  - BACKGROUND: Soft tissue sarcomas (STS) are a heterogeneous group of tumors. For 
      adequate therapeutic management, an accurate diagnosis is necessary. In Morocco, 
      the diagnosis is essentially based on the morphological and immunohistochemical 
      study. Compared to other techniques, fluorescence in situ hybridization (FISH) is 
      easier to develop and less expensive. This study aims to assess the feasibility 
      and utility of implementing FISH technique to improve diagnostic accuracy and 
      establish a good classification. MATERIAL AND METHODS: This is a retrospective 
      cohort study. 211 cases of mesenchymal tumors were included. Hematoxylin Eosin 
      Safran (HES) staining was performed in all cases followed by immunohistochemistry 
      (IHC). FISH was performed in all cases with suspected STS. The probes used were 
      EWSR1, MDM2 and SS18. The performance of FISH and histopathological test were 
      evaluated by the ROC curve method (receiver operating characteristic). We 
      evaluated the concordance between FISH and real time PCR by Cohen test. RESULTS: 
      The real-time PCR technique showed good agreement with the FISH test by a Kappa 
      coefficient of 60% (p = 0.035). FISH was able to confirm that it is more accurate 
      (Youden's Index = 91%) than histological/immunohistochemical analysis (Youden's 
      Index = 51%), as well as the positive predictive value was higher (100%) with an 
      ROC curve finding a larger area under the curve of 0.953 (95% CI: 0.918-0.988), p 
      = 0.000 which supports that FISH shows high performance to present an accurate 
      final diagnosis. CONCLUSION: This is the first and the largest Moroccan series 
      for the molecular diagnosis of STS by FISH. Our study shows that paraffin FISH is 
      a sensitive and specific ancillary tool in the diagnosis of STS when used in the 
      appropriate clinicopathological context.
CI  - (c) 2022 The Author(s).
FAU - El Koubaiti, Rhizlane
AU  - El Koubaiti R
AD  - Biomedical and Translational Research Laboratory, Faculty of Medicine and 
      Pharmacy, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
FAU - Mazti, Asmae
AU  - Mazti A
AD  - Pathology Department. Hassan II University Hospital, Faculty of Medicine and 
      Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
FAU - Maaroufi, Mustapha
AU  - Maaroufi M
AD  - Department of Radiology, Hassan II University Hospital, Faculty of Medicine and 
      Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
FAU - El Idrissi, Mohammed
AU  - El Idrissi M
AD  - Department of Trauma-orthopedics B4, Hassan II University Hospital, Faculty of 
      Medicine and Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
FAU - El Ibrahimi, Abdelhalim
AU  - El Ibrahimi A
AD  - Department of Trauma-orthopedics B4, Hassan II University Hospital, Faculty of 
      Medicine and Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
FAU - El Mrini, Abdelmajid
AU  - El Mrini A
AD  - Department of Trauma-orthopedics B4, Hassan II University Hospital, Faculty of 
      Medicine and Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
FAU - Bouhafa, Touria
AU  - Bouhafa T
AD  - Department of Radiotherapy, Hassan II University Hospital, Faculty of Medicine 
      and Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
FAU - El Fakir, Samira
AU  - El Fakir S
AD  - Department of Epidemiology and Public Health, Hassan II University Hospital, 
      Faculty of Medicine and Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, 
      Morocco.
FAU - Ouldim, Karim
AU  - Ouldim K
AD  - Laboratory of Medical Genetics and Oncogenetics, HASSAN II University Hospital, 
      Fez, Morocco.
FAU - Arifi, Samia
AU  - Arifi S
AD  - Medical Oncology Department, Faculty of Medicine and Pharmacy Fez, Sidi Mohamed 
      Ben Abdellah University, Fez, Morocco.
FAU - Chbani, Laila
AU  - Chbani L
AD  - Pathology Department. Hassan II University Hospital, Faculty of Medicine and 
      Pharmacy Fez, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
LA  - eng
PT  - Journal Article
DEP - 20220917
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9508571
OTO - NOTNLM
OT  - Diagnosis
OT  - Fluorescence in situ Hybridization
OT  - Molecular classification
OT  - Soft tissue sarcomas
COIS- The authors declare no conflict of interest.
EDAT- 2022/09/28 06:00
MHDA- 2022/09/28 06:01
PMCR- 2022/09/17
CRDT- 2022/09/27 02:01
PHST- 2022/02/09 00:00 [received]
PHST- 2022/06/24 00:00 [revised]
PHST- 2022/09/12 00:00 [accepted]
PHST- 2022/09/27 02:01 [entrez]
PHST- 2022/09/28 06:00 [pubmed]
PHST- 2022/09/28 06:01 [medline]
PHST- 2022/09/17 00:00 [pmc-release]
AID - S2405-8440(22)01961-2 [pii]
AID - e10673 [pii]
AID - 10.1016/j.heliyon.2022.e10673 [doi]
PST - epublish
SO  - Heliyon. 2022 Sep 17;8(9):e10673. doi: 10.1016/j.heliyon.2022.e10673. eCollection 
      2022 Sep.