PMID- 36164624
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221004
IS  - 2322-3480 (Print)
IS  - 2322-3480 (Electronic)
IS  - 2322-3480 (Linking)
VI  - 11
IP  - 2
DP  - 2022 Jul
TI  - Association of Tumor Necrosis Factor-alpha and Myeloperoxidase enzyme with Severe 
      Asthma: A comparative study.
PG  - 238-245
LID - 10.52547/rbmb.11.2.238 [doi]
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) may stimulate airway 
      hyperresponsiveness in asthma, which is also affected by neutrophils activity. 
      The latter can be determined indirectly by evaluating myeloperoxidase (MPO) 
      activity. The insufficient studies that investigated the combined association of 
      serum TNF-alpha and MPO with asthma was objective of this study. METHODS: A 
      case-control study included 110-asthmatics besides 92-controls. All participants 
      underwent venous sampling for TNF-alpha and MPO immunoassays. A percentage of 
      predicted "forced expiratory volume in one second (FEV1%)", and the "peak 
      expiratory flow rate (PEF/L)" of all participants were verified. The statistical 
      analyses had done using SPSS V-25. The accuracy, specificity, sensitivity, and 
      significance of both biomarkers to distinguish asthma examined "under the 
      ROC-curves". RESULTS: High TNF-alpha levels observed among the controls(p-0.006), 
      opposing the higher MPO levels among the patients(p-0.00). There were 
      nonsignificant variations of two biomarkers between the treatment groups and 
      nonsignificant correlations of MPO with FEV1 and PEF. There was a significant 
      correlation of MPO with the TNF-alpha levels of all participants. The TNF-alpha showed 
      lower sensitivity, specificity, and accuracy to diagnose asthma. There were no 
      MPO differences according to asthma levels. The TNF-alpha was higher among the severe 
      asthmatics significantly. DISCUSSION: TNF-alpha may be a contributory particle for 
      neutrophilic inflammation of severe asthma. MPO levels were significantly higher 
      among asthmatics, whereas TNF-alpha levels were lower. TNF-alpha levels were higher among 
      those with severe compared to mild/moderate asthma. The MPO level has a 
      significant predictive capacity compared to TNF-alpha for distinguishing asthma from 
      healthy subjects.
FAU - Aldhalmi, Ahmed Khalid
AU  - Aldhalmi AK
AD  - The University of Mashreq, College of Pharmacy, Baghdad, Iraq.
FAU - Al-Athari, Ali Jihad Hemid
AU  - Al-Athari AJH
AD  - Al-Mustaqbal University College, Department of Pharmacy, Iraq.
FAU - Makki Al-Hindy, Hayder Abdul-Amir
AU  - Makki Al-Hindy HA
AD  - College of Pharmacy, University of Babylon, Babylon, Iraq.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Rep Biochem Mol Biol
JT  - Reports of biochemistry & molecular biology
JID - 101637937
PMC - PMC9455177
OTO - NOTNLM
OT  - Asthma
OT  - Inflammation
OT  - MPO
OT  - Neutrophils
OT  - TNF-alpha
EDAT- 2022/09/28 06:00
MHDA- 2022/09/28 06:01
PMCR- 2022/07/01
CRDT- 2022/09/27 02:04
PHST- 2021/11/20 00:00 [received]
PHST- 2022/11/22 00:00 [accepted]
PHST- 2022/09/27 02:04 [entrez]
PHST- 2022/09/28 06:00 [pubmed]
PHST- 2022/09/28 06:01 [medline]
PHST- 2022/07/01 00:00 [pmc-release]
AID - rbmb-11-238 [pii]
AID - 10.52547/rbmb.11.2.238 [doi]
PST - ppublish
SO  - Rep Biochem Mol Biol. 2022 Jul;11(2):238-245. doi: 10.52547/rbmb.11.2.238.