PMID- 36166647
OWN - NLM
STAT- MEDLINE
DCOM- 20221007
LR  - 20221011
IS  - 1948-7185 (Electronic)
IS  - 1948-7185 (Linking)
VI  - 13
IP  - 39
DP  - 2022 Oct 6
TI  - Effects of Interfacial Interactions on Electrocatalytic Activity of Cytochrome c 
      Oxidase in Biomimetic Lipid Membranes on Gold Electrodes.
PG  - 9165-9170
LID - 10.1021/acs.jpclett.2c01765 [doi]
AB  - Effects of interfacial interactions on the electrocatalytic activity of 
      protein-tethered bilayer lipid membranes (ptBLMs) containing cytochrome c oxidase 
      (CcO) for the oxygen reduction reaction are studied by using protein film 
      electrochemistry and surface-enhanced infrared absorption (SEIRA) spectroscopy. 
      Mammalian CcO was immobilized on a gold electrode via self-assembled monolayers 
      (SAMs) of mixed alkanethiols. The protein orientation on the electrode is 
      controlled by SAM-CcO interactions and is critical to the cytochrome c (cyt c) 
      binding. The CcO-phospholipid and CcO-cyt c interactions modulate the 
      electrocatalytic activity of CcO, and more densely packed ptBLMs show higher 
      electrocatalytic activity. Our study indicates that spectroscopic and 
      electrochemical studies of ptBLMs can provide insights into the effects of 
      relatively weak protein-protein and protein-lipid interactions on the enzymatic 
      activity of transmembrane enzymes.
FAU - Kato, Masaru
AU  - Kato M
AUID- ORCID: 0000-0002-2845-3525
AD  - Faculty of Environmental Earth Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
AD  - Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
FAU - Sano, Ryoya
AU  - Sano R
AD  - Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
FAU - Yoshida, Narumi
AU  - Yoshida N
AD  - Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
FAU - Iwafuji, Masatoshi
AU  - Iwafuji M
AD  - Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
FAU - Nishiyama, Yoshito
AU  - Nishiyama Y
AD  - Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
FAU - Oka, Sayuki
AU  - Oka S
AD  - Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
FAU - Shinzawa-Itoh, Kyoko
AU  - Shinzawa-Itoh K
AD  - Graduate School of Science, University of Hyogo, Ako, Hyogo 678-1297, Japan.
FAU - Nishida, Yuya
AU  - Nishida Y
AD  - Department of Molecular Pharmacology, National Cerebral and Cardiovascular 
      Center, Suita, Osaka 564-8565, Japan.
FAU - Shintani, Yasunori
AU  - Shintani Y
AD  - Department of Molecular Pharmacology, National Cerebral and Cardiovascular 
      Center, Suita, Osaka 564-8565, Japan.
FAU - Yagi, Ichizo
AU  - Yagi I
AUID- ORCID: 0000-0002-4005-9440
AD  - Faculty of Environmental Earth Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
AD  - Graduate School of Environmental Science, Hokkaido University, N10W5, Kita-ku, 
      Sapporo 060-0810, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220927
PL  - United States
TA  - J Phys Chem Lett
JT  - The journal of physical chemistry letters
JID - 101526034
RN  - 0 (Lipid Bilayers)
RN  - 0 (Phospholipids)
RN  - 7440-57-5 (Gold)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Biomimetics
MH  - Cytochromes c
MH  - Electrodes
MH  - *Electron Transport Complex IV/chemistry/metabolism
MH  - *Gold/chemistry
MH  - Lipid Bilayers
MH  - Mammals/metabolism
MH  - Oxygen/metabolism
MH  - Phospholipids
EDAT- 2022/09/28 06:00
MHDA- 2022/10/12 06:00
CRDT- 2022/09/27 14:53
PHST- 2022/09/28 06:00 [pubmed]
PHST- 2022/10/12 06:00 [medline]
PHST- 2022/09/27 14:53 [entrez]
AID - 10.1021/acs.jpclett.2c01765 [doi]
PST - ppublish
SO  - J Phys Chem Lett. 2022 Oct 6;13(39):9165-9170. doi: 10.1021/acs.jpclett.2c01765. 
      Epub 2022 Sep 27.