PMID- 36169894 OWN - NLM STAT- MEDLINE DCOM- 20221205 LR - 20221205 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 49 IP - 12 DP - 2022 Dec TI - Two missense variants of the epidermal growth factor receptor gene are associated with non small cell lung carcinoma in the subjects from Iraq. PG - 11653-11661 LID - 10.1007/s11033-022-07933-w [doi] AB - BACKGROUND: Lung carcinoma is a foremost cause of cancer-related mortality worldwide. Variable genetic factors are associated with the development of lung cancer. This study was performed to evaluate the possible association of epidermal growth factor receptor (EGFR) gene polymorphisms with non small cell lung carcinoma (NSCLC) in Iraqi population. METHODS: DNA samples were extracted from 100 patients and 100 controls. Four PCR fragments were designed to amplify four high-frequency variants within EGFR, namely rs1050171, rs2072454, rs2227984, and rs2227983. The PCR fragments were genotyped by single-strand conformation polymorphism (SSCP) method, and each genotype was subjected to direct sequencing. RESULTS: Genotyping experiments confirmed the variability of three targeted variants, and logistic regression analysis showed that two of these variants (rs1050171 and rs2227983) tend to exhibit a significant association with NSCLC. Individuals with rs1050171:GA genotype showed a possible association with the increased risk of NSCLC (P = 0.0110; OD 5.2636; Cl(95%) 1.4630 to 18.9371). Individuals with rs2227983:GG genotype exhibited a potential association with NSCLC (P = 0.0037; OD 5.2683; Cl(95%) 1.7141 to 16.1919). Linkage disequilibrium analysis showed that the effects of the investigated variants seem to take independent actions, and no haplotype was found to be associated with the high prevalence of NSCLC. CONCLUSIONS: Our collective data indicated that EGFR-rs1050171G/A and EGFR-rs2227983G/G SNPs tend to exert significant and separate associations with the increased risk of NSCLC. However, this study recommends using a broader spectrum of the investigated samples to get further details of both SNPs in terms of their association with the susceptibility to NSCLC. CI - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Lawi, Zahraa K AU - Lawi ZK AUID- ORCID: 0000-0001-8135-7957 AD - Department of Biology, College of Science, University of Kufa, Najaf, 54001, Iraq. FAU - Al-Shuhaib, Mohammed Baqur S AU - Al-Shuhaib MBS AUID- ORCID: 0000-0002-6458-2068 AD - Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, 51001, Iraq. mohammed79@agre.uoqasim.edu.iq. FAU - Amara, Ibtissem Ben AU - Amara IB AUID- ORCID: 0000-0003-3027-2747 AD - Laboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of SFAX, PB 261, 3000, Sfax, Tunisia. FAU - Alkhammas, Ahmed H AU - Alkhammas AH AUID- ORCID: 0000-0002-4625-9881 AD - Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil, 51001, Iraq. LA - eng PT - Journal Article DEP - 20220928 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - EC 2.7.10.1 (ErbB Receptors) RN - EC 2.7.10.1 (EGFR protein, human) SB - IM MH - Humans MH - *Carcinoma, Non-Small-Cell Lung/genetics/pathology MH - Case-Control Studies MH - *ErbB Receptors/genetics MH - Genes, erbB-1 MH - Iraq MH - *Lung Neoplasms/genetics/pathology OTO - NOTNLM OT - EGFR OT - NSCLC OT - Variations OT - rs1050171 OT - rs2227983 EDAT- 2022/09/29 06:00 MHDA- 2022/12/03 06:00 CRDT- 2022/09/28 11:22 PHST- 2022/06/24 00:00 [received] PHST- 2022/09/07 00:00 [accepted] PHST- 2022/09/29 06:00 [pubmed] PHST- 2022/12/03 06:00 [medline] PHST- 2022/09/28 11:22 [entrez] AID - 10.1007/s11033-022-07933-w [pii] AID - 10.1007/s11033-022-07933-w [doi] PST - ppublish SO - Mol Biol Rep. 2022 Dec;49(12):11653-11661. doi: 10.1007/s11033-022-07933-w. Epub 2022 Sep 28.