PMID- 36171339
OWN - NLM
STAT- MEDLINE
DCOM- 20220930
LR  - 20221128
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Sep 28
TI  - Biosynthesis of copper oxide nanoparticles mediated Annona muricata as cytotoxic 
      and apoptosis inducer factor in breast cancer cell lines.
PG  - 16165
LID - 10.1038/s41598-022-20360-y [doi]
LID - 16165
AB  - This study investigated for the first time a simple bio-synthesis approach for 
      the synthesis of copper oxide nanoparticles (CuO NPs) using Annona muricata L (A. 
      muricata) plant extract to test their anti-cancer effects. The presence of CuONPs 
      was confirmed by UV-visible spectroscopy, Scanning electron microscope (SEM), and 
      Transmission electron microscope (TEM). The antiproliferative properties of the 
      synthesized nanoparticles were evaluated against (AMJ-13), (MCF-7) breast cancer 
      cell lines, and the human breast epithelial cell line (HBL-100) as healthy cells. 
      This study indicates that CuONPs reduced cell proliferation for AMJ-13 and MCF-7. 
      HBL-100 cells were not significantly inhibited for several concentration levels 
      or test periods. The outcomes suggest that the prepared copper oxide 
      nanoparticles acted against the growth of specific cell lines observed in breast 
      cancer. It was observed that cancer cells had minor colony creation after 24 h 
      sustained CuONPs exposure using (IC(50)) concentration for AMJ-13 was 
      (17.04 microg mL(-1)). While for MCF-7 cells was (18.92 microg mL(-1)). It indicates the 
      uptake of CuONPs by cancer cells, triggering apoptosis. Moreover, treatment with 
      CuONPs enhanced Lactate dehydrogenase (LDH) production, probably caused by cell 
      membrane damage, creating leaks comprising cellular substances like lactate 
      dehydrogenase. Hence, research results suggested that the synthesized CuONPs 
      precipitated anti-proliferative effects by triggering cell death through 
      apoptosis.
CI  - (c) 2022. The Author(s).
FAU - Mahmood, Rana I
AU  - Mahmood RI
AUID- ORCID: 0000-0001-6498-6049
AD  - Department of Biomedical Engineering, College of Engineering, Al-Nahrain 
      University, Baghdad, Iraq.
FAU - Kadhim, Afraa Ali
AU  - Kadhim AA
AD  - Department of Biology, College of Science, Mustansiriyah University, Baghdad, 
      Iraq.
FAU - Ibraheem, Sumayah
AU  - Ibraheem S
AD  - Al_kindy College of Medicine, University of Baghdad, Baghdad, Iraq.
FAU - Albukhaty, Salim
AU  - Albukhaty S
AUID- ORCID: 0000-0001-7885-4215
AD  - Department of Chemistry, College of Science, University of Misan, Maysan, 62001, 
      Iraq. albukhaty.salim@uomisan.edu.iq.
AD  - College of Medicine, University of Warith Al-Anbiyaa, Karbala, Iraq. 
      albukhaty.salim@uomisan.edu.iq.
FAU - Mohammed-Salih, Harraa S
AU  - Mohammed-Salih HS
AUID- ORCID: 0000-0001-7680-9437
AD  - Department of Orthodontics, College of Dentistry, University of Baghdad, Baghdad, 
      10011, Iraq.
FAU - Abbas, Ruaa H
AU  - Abbas RH
AD  - College of Dentistry, Al-Farahidi University, Baghdad, Iraq.
FAU - Jabir, Majid S
AU  - Jabir MS
AUID- ORCID: 0000-0003-0759-8298
AD  - Department of Applied Sciences, University of Technology, Baghdad, 10066, Iraq. 
      100131@uotechnology.edu.iq.
FAU - Mohammed, Mustafa K A
AU  - Mohammed MKA
AD  - Department of Medical Physics, Al-Mustaqbal University College, Babylon, 51001, 
      Iraq.
FAU - Nayef, Uday M
AU  - Nayef UM
AD  - Department of Applied Sciences, University of Technology, Baghdad, 10066, Iraq.
FAU - AlMalki, Faizah A
AU  - AlMalki FA
AUID- ORCID: 0000-0002-3602-5889
AD  - Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif, 
      21944, Saudi Arabia.
FAU - Sulaiman, Ghassan M
AU  - Sulaiman GM
AD  - Department of Applied Sciences, University of Technology, Baghdad, 10066, Iraq.
FAU - Al-Karagoly, Hassan
AU  - Al-Karagoly H
AUID- ORCID: 0000-0002-2199-9322
AD  - Department of Internal and Preventive Medicine, College of Veterinary Medicine, 
      University of Al-Qadisiyah, Al Diwaniyah, 58002, Iraq.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220928
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Oxides)
RN  - 0 (Plant Extracts)
RN  - 789U1901C5 (Copper)
RN  - EC 1.1.- (Lactate Dehydrogenases)
RN  - T8BEA5064F (cuprous oxide)
SB  - IM
MH  - *Annona/chemistry
MH  - *Antineoplastic Agents/pharmacology
MH  - Apoptosis
MH  - *Breast Neoplasms/drug therapy
MH  - Cell Line
MH  - Copper/chemistry
MH  - Female
MH  - Humans
MH  - Lactate Dehydrogenases
MH  - *Metal Nanoparticles/chemistry
MH  - *Nanoparticles/chemistry
MH  - Oxides/pharmacology
MH  - Plant Extracts/chemistry/pharmacology
PMC - PMC9519583
COIS- The authors declare no competing interests.
EDAT- 2022/09/29 06:00
MHDA- 2022/10/01 06:00
PMCR- 2022/09/28
CRDT- 2022/09/28 23:27
PHST- 2022/05/13 00:00 [received]
PHST- 2022/09/12 00:00 [accepted]
PHST- 2022/09/28 23:27 [entrez]
PHST- 2022/09/29 06:00 [pubmed]
PHST- 2022/10/01 06:00 [medline]
PHST- 2022/09/28 00:00 [pmc-release]
AID - 10.1038/s41598-022-20360-y [pii]
AID - 20360 [pii]
AID - 10.1038/s41598-022-20360-y [doi]
PST - epublish
SO  - Sci Rep. 2022 Sep 28;12(1):16165. doi: 10.1038/s41598-022-20360-y.