PMID- 36172263
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220930
IS  - 1662-5099 (Print)
IS  - 1662-5099 (Electronic)
IS  - 1662-5099 (Linking)
VI  - 15
DP  - 2022
TI  - The feedback loop between calcineurin, calmodulin-dependent protein kinase II, 
      and nuclear factor of activated T-cells regulates the number of GABAergic neurons 
      during planarian head regeneration.
PG  - 988803
LID - 10.3389/fnmol.2022.988803 [doi]
LID - 988803
AB  - Disturbances in the excitatory/inhibitory balance of brain neural circuits are 
      the main source of encephalopathy during neurodevelopment. Changes in the 
      function of neural circuits can lead to depolarization or repeat rhythmic firing 
      of neurons in a manner similar to epilepsy. GABAergic neurons are inhibitory 
      neurons found in all the main domains of the CNS. Previous studies suggested that 
      DjCamkII and DjCaln play a crucial role in the regulation of GABAergic neurons 
      during planarian regeneration. However, the mechanisms behind the regeneration of 
      GABAergic neurons have not been fully explained. Herein, we demonstrated that 
      DjCamkII and DjCaln were mutual negative regulation during planarian head 
      regeneration. DjNFAT exerted feedback positive regulation on both DjCaln and 
      DjCamkII. Whole-mount in situ hybridization (WISH) and fluorescence in situ 
      hybridization (FISH) revealed that DjNFAT was predominantly expressed in the 
      pharynx and parenchymal cells in intact planarian. Interestingly, during 
      planarian head regeneration, DjNFAT was predominantly located in the newborn 
      brain. Down-regulation of DjNFAT led to regeneration defects in the brain 
      including regenerative brain became small and the lateral nerves cannot be 
      regenerated completely, and a decreasein the number of GABAergic neurons during 
      planarian head regeneration. These findings suggest that the feedback loop 
      between DjCaln, DjCamkII, and DjNFAT is crucial for the formation of GABAergic 
      neurons during planarian head regeneration.
CI  - Copyright (c) 2022 Zhen, Zheng, Geng, Song, Gao, Yuan, Deng, Pang and Zhao.
FAU - Zhen, Hui
AU  - Zhen H
AD  - Laboratory of Developmental and Evolutionary Biology, Shandong University of 
      Technology, Nantong, China.
FAU - Zheng, Mingyue
AU  - Zheng M
AD  - Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, 
      Guangzhou, China.
FAU - Geng, Huazhi
AU  - Geng H
AD  - Zibo Maternal and Child Health Hospital, Zibo, China.
FAU - Song, Qian
AU  - Song Q
AD  - Laboratory of Developmental and Evolutionary Biology, Shandong University of 
      Technology, Nantong, China.
FAU - Gao, Lili
AU  - Gao L
AD  - Laboratory of Developmental and Evolutionary Biology, Shandong University of 
      Technology, Nantong, China.
FAU - Yuan, Zuoqing
AU  - Yuan Z
AD  - Laboratory of Developmental and Evolutionary Biology, Shandong University of 
      Technology, Nantong, China.
FAU - Deng, Hongkuan
AU  - Deng H
AD  - Laboratory of Developmental and Evolutionary Biology, Shandong University of 
      Technology, Nantong, China.
FAU - Pang, Qiuxiang
AU  - Pang Q
AD  - Laboratory of Developmental and Evolutionary Biology, Shandong University of 
      Technology, Nantong, China.
FAU - Zhao, Bosheng
AU  - Zhao B
AD  - Laboratory of Developmental and Evolutionary Biology, Shandong University of 
      Technology, Nantong, China.
LA  - eng
PT  - Journal Article
DEP - 20220912
PL  - Switzerland
TA  - Front Mol Neurosci
JT  - Frontiers in molecular neuroscience
JID - 101477914
PMC - PMC9510629
OTO - NOTNLM
OT  - Caln
OT  - CamkII
OT  - GABAergic neurons
OT  - NFAT
OT  - planarian
OT  - regeneration
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/09/30 06:00
MHDA- 2022/09/30 06:01
PMCR- 2022/01/01
CRDT- 2022/09/29 02:15
PHST- 2022/07/07 00:00 [received]
PHST- 2022/08/24 00:00 [accepted]
PHST- 2022/09/29 02:15 [entrez]
PHST- 2022/09/30 06:00 [pubmed]
PHST- 2022/09/30 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnmol.2022.988803 [doi]
PST - epublish
SO  - Front Mol Neurosci. 2022 Sep 12;15:988803. doi: 10.3389/fnmol.2022.988803. 
      eCollection 2022.