PMID- 36173310
OWN - NLM
STAT- MEDLINE
DCOM- 20221109
LR  - 20221109
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 10
IP  - 22
DP  - 2022 Nov 8
TI  - Strategy of a cell-derived extracellular matrix for the construction of an 
      osteochondral interlayer.
PG  - 6472-6485
LID - 10.1039/d2bm01230h [doi]
AB  - Osteochondral defects pose an enormous challenge due to the lack of an effective 
      repair strategy. To tackle this issue, the importance of a calcified cartilage 
      interlayer (CCL) in modulating osteochondral regeneration should be valued. 
      Herein, we proposed that an extracellular matrix (ECM) derived from a suitable 
      cell source might efficiently promote the formation of calcified cartilage. To 
      the end, cell sheets from four kinds of cells, including bone marrow mesenchymal 
      stem cells (BMSCs), pre-osteoblasts (MC3T3), chondrocytes (Cho), and artificially 
      induced hypertrophic chondrocytes (HCho), were obtained by seeding the cells on 
      electrospun fibrous meshes, followed by decellularization to prepare 
      decellularized ECMs (D-ECMs) for BMSC re-seeding and differentiation studies. For 
      cell proliferation, the BMSC-derived D-ECM exhibited the strongest promotion 
      effect. For inducing the hypertrophic phenotype of re-seeded BMSCs, both the 
      BMSC-derived and HCho-derived D-ECMs demonstrated stronger capacity in 
      up-regulating the depositions of related proteins and the expressions of marker 
      genes, as compared to the MC3T3-derived and Cho-derived D-ECMs. Accordingly, from 
      the histological results of their subcutaneous implantation in rats, both the 
      BMSC-derived and HCho-derived D-ECMs displayed obvious Masson's trichrome and 
      Safranin-O/Fast-Green staining colors simultaneously, representing the 
      characteristics related to osteogenesis and chondrogenesis. Differently, 
      MC3T3-derived and Cho-derived D-ECMs were mainly detected during the osteogenic 
      or chondrogenic expression, respectively. These findings confirmed that the 
      BMSC-derived D-ECM could induce hypertrophic chondrocytes, though being a little 
      inferior to the HCho-derived D-ECM. Overall, the BMSC-derived D-ECM could be a 
      potential material in constructing the interlayer for osteochondral tissue 
      engineering scaffolds to improve the regeneration efficiency.
FAU - Gao, Chenyuan
AU  - Gao C
AD  - State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of 
      Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, 
      China. caiqing@mail.buct.edu.cn.
FAU - Fu, Lei
AU  - Fu L
AD  - State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of 
      Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, 
      China. caiqing@mail.buct.edu.cn.
FAU - Yu, Yingjie
AU  - Yu Y
AUID- ORCID: 0000-0002-2543-9808
AD  - State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of 
      Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, 
      China. caiqing@mail.buct.edu.cn.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Institute of Sports Medicine, Beijing Key Laboratory of Sports Injuries, Peking 
      University Third Hospital, Beijing 100191, People's Republic of China. 
      18600622016@163.com.
FAU - Yang, Xiaoping
AU  - Yang X
AD  - State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of 
      Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, 
      China. caiqing@mail.buct.edu.cn.
AD  - Foshan (Southern China) Institute for New Materials, Foshan 528200, Guangdong, 
      China.
FAU - Cai, Qing
AU  - Cai Q
AUID- ORCID: 0000-0001-6618-0321
AD  - State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of 
      Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, 
      China. caiqing@mail.buct.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20221108
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Chondrogenesis
MH  - Extracellular Matrix/metabolism
MH  - Chondrocytes
MH  - *Mesenchymal Stem Cells
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds
MH  - Cell Differentiation/physiology
EDAT- 2022/09/30 06:00
MHDA- 2022/11/10 06:00
CRDT- 2022/09/29 10:06
PHST- 2022/09/30 06:00 [pubmed]
PHST- 2022/11/10 06:00 [medline]
PHST- 2022/09/29 10:06 [entrez]
AID - 10.1039/d2bm01230h [doi]
PST - epublish
SO  - Biomater Sci. 2022 Nov 8;10(22):6472-6485. doi: 10.1039/d2bm01230h.