PMID- 36175229 OWN - NLM STAT- MEDLINE DCOM- 20221229 LR - 20221229 IS - 1525-3198 (Electronic) IS - 0022-0302 (Linking) VI - 105 IP - 11 DP - 2022 Nov TI - Feeding tannins to dairy cows in different seasons improves the oxidative status of blood plasma and the antioxidant capacity of cheese. PG - 8609-8620 LID - S0022-0302(22)00558-6 [pii] LID - 10.3168/jds.2022-22256 [doi] AB - The aim of the present study was to assess the dietary supplementation of tannins to grazing dairy cows in 2 seasons characterized by a good quality pasture (spring) or a poor-quality pasture (summer). The effects of dietary tannins were assessed on plasma antioxidant status and cytokines profile and on the antioxidant properties of cheese and cheese in vitro digestates. Fourteen lactating dairy cows were divided into 2 homogeneous groups (n = 7): a control group (CON), and an experimental group (TAN) receiving 150 g/head per day of tannins supplementation. The experiment was performed twice, in spring and in summer. The animals were free to graze on spontaneous pasture (spring) or on dry stubble (summer). Blood was sampled at the beginning (d 0), at the midpoint (d 11), and at the end (d 22) of the trial. Individual cheese was produced before the beginning (d -1) and at the end (d 22) of the trial from the milk collected from each cow. On blood plasma, the reactive oxygen metabolites (ROM), biological antioxidant potential (BAP), nonesterified fatty acids quantification, and cytokines profile in terms of IL-10, IL-8, IL-1beta, and IFN-gamma were determined. Data on ROM demonstrated that tannins supplementation lowered oxidative stress both in spring and in summer. Accordingly, TAN diet increased BAP levels compared with the CON during summer trial. Thus, feeding tannins resulted in lower ratio between ROM and BAP (oxidative stress index) in both spring and summer. Cytokines' profile showed lower IL-1beta values in TAN group at d 22 during spring season, with a concomitant higher IL-10 level, during summer season. Moreover, TAN group had a lower level of IFN-gamma in plasma than CON group, both in spring and in summer. On cheese samples, the in vitro digestion was performed and on cheese and cheese digestates (gastric and intestinal digestate) the free radical scavenging antioxidant activity was evaluated. The intestinal digestate fraction registered the highest antioxidant activity compared with cheese and gastric digestate, in both spring and summer seasons. Furthermore, an improvement of the antioxidant property of cheese and cheese digestates was found. Present data demonstrated that tannins supplementation contributed to reduce the oxidative stress of lactating dairy cows and showed an increase of anti-inflammatory cytokines ratio. CI - The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association(R). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). FAU - Santillo, A AU - Santillo A AD - Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: antonella.santillo@unifg.it. FAU - Ciliberti, M G AU - Ciliberti MG AD - Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. FAU - Ciampi, F AU - Ciampi F AD - Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. FAU - Luciano, G AU - Luciano G AD - Department Di3A, University of Catania, via Valdisavoia 5, 95123 Catania, Italy. FAU - Natalello, A AU - Natalello A AD - Department Di3A, University of Catania, via Valdisavoia 5, 95123 Catania, Italy. FAU - Menci, R AU - Menci R AD - Department Di3A, University of Catania, via Valdisavoia 5, 95123 Catania, Italy. FAU - Caccamo, M AU - Caccamo M AD - Consorzio per la Ricerca nel settore della Filiera Lattiero-Casearia e dell'agroalimentare (CoRFiLaC), Regione Siciliana, 97100 Ragusa, Italy. FAU - Sevi, A AU - Sevi A AD - Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. FAU - Albenzio, M AU - Albenzio M AD - Department of Agriculture, Food, Natural Resources, and Engineering (DAFNE), University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. LA - eng PT - Journal Article DEP - 20220927 PL - United States TA - J Dairy Sci JT - Journal of dairy science JID - 2985126R RN - 0 (Antioxidants) RN - 0 (Fatty Acids, Nonesterified) RN - 130068-27-8 (Interleukin-10) RN - 0 (Interleukin-8) RN - S88TT14065 (Oxygen) RN - 0 (Tannins) SB - IM MH - Animals MH - Cattle MH - Female MH - *Animal Feed/analysis MH - Antioxidants/metabolism MH - *Cheese/analysis MH - Diet/veterinary MH - Fatty Acids, Nonesterified/metabolism MH - Interleukin-10/metabolism MH - Interleukin-8/metabolism MH - Lactation MH - Milk/metabolism MH - Oxidative Stress MH - Oxygen/metabolism MH - Plasma MH - Seasons MH - *Tannins/administration & dosage OTO - NOTNLM OT - cheese digestate antioxidant capacity OT - cheese quality OT - immunity OT - pasture EDAT- 2022/09/30 06:00 MHDA- 2022/10/26 06:00 CRDT- 2022/09/29 22:04 PHST- 2022/05/02 00:00 [received] PHST- 2022/06/30 00:00 [accepted] PHST- 2022/09/30 06:00 [pubmed] PHST- 2022/10/26 06:00 [medline] PHST- 2022/09/29 22:04 [entrez] AID - S0022-0302(22)00558-6 [pii] AID - 10.3168/jds.2022-22256 [doi] PST - ppublish SO - J Dairy Sci. 2022 Nov;105(11):8609-8620. doi: 10.3168/jds.2022-22256. Epub 2022 Sep 27.