PMID- 36175402
OWN - NLM
STAT- MEDLINE
DCOM- 20221003
LR  - 20221222
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 14
IP  - 1
DP  - 2022 Jan-Dec
TI  - Visualizing germination of microbiota endospores in the mammalian gut.
PG  - 2125737
LID - 10.1080/19490976.2022.2125737 [doi]
LID - 2125737
AB  - Transmission of bacterial endospores between the environment and people and the 
      following germination in vivo play critical roles in both the deadly infections 
      of some bacterial pathogens and the stabilization of the commensal microbiotas in 
      humans. Our knowledge about the germination process of different bacteria in the 
      mammalian gut, however, is still very limited due to the lack of suitable tools 
      to visually monitor this process. We proposed a two-step labeling strategy that 
      can image and quantify the endospores' germination in the recipient's intestines. 
      Endospores collected from donor's gut microbiota were first labeled with 
      fluorescein isothiocyanate and transplanted to mice via gavage. The recipient 
      mice were then administered with Cyanine5-tagged D-amino acid to label all the 
      viable bacteria, including the germinated endospores, in their intestines in 
      situ. The germinated donor endospores could be distinguished by presenting two 
      types of fluorescent signals simultaneously. The integrative use of cell-sorting, 
      16S rDNA sequencing, and fluorescence in situ hybridization (FISH) staining of 
      the two-colored bacteria unveiled the taxonomic information of the donor 
      endospores that germinated in the recipient's gut. Using this strategy, we 
      investigated effects of different germinants and pre-treatment interventions on 
      their germination, and found that germination of different commensal bacterial 
      genera was distinctly affected by various types of germinants. This two-color 
      labeling strategy shows its potential as a versatile tool for visually monitoring 
      endospore germination in the hosts and screening for new interventions to improve 
      endospore-based therapeutics.
FAU - Xu, Ningning
AU  - Xu N
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
FAU - Lin, Liyuan
AU  - Lin L
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
FAU - Du, Yahui
AU  - Du Y
AD  - The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key 
      Laboratory for Chemical Biology of Fujian Province State Key Laboratory of 
      Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of 
      Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.
FAU - Lin, Huibin
AU  - Lin H
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
FAU - Song, Jia
AU  - Song J
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
FAU - Yang, Chaoyong
AU  - Yang C
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
AD  - The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key 
      Laboratory for Chemical Biology of Fujian Province State Key Laboratory of 
      Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of 
      Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.
FAU - Wang, Wei
AU  - Wang W
AUID- ORCID: 0000-0002-5539-4532
AD  - Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid 
      Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
RN  - 0 (Amino Acids)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Isothiocyanates)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Amino Acids
MH  - Animals
MH  - DNA, Ribosomal
MH  - Fluorescein
MH  - *Gastrointestinal Microbiome
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Isothiocyanates
MH  - Mammals
MH  - Mice
MH  - *Microbiota
MH  - Spores, Bacterial
PMC - PMC9543051
OTO - NOTNLM
OT  - Bacterial endospore
OT  - FDAA
OT  - germination
OT  - in vivo metabolic labeling
OT  - two-color fluorescence imaging
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/09/30 06:00
MHDA- 2022/10/04 06:00
PMCR- 2022/09/29
CRDT- 2022/09/29 23:14
PHST- 2022/09/29 23:14 [entrez]
PHST- 2022/09/30 06:00 [pubmed]
PHST- 2022/10/04 06:00 [medline]
PHST- 2022/09/29 00:00 [pmc-release]
AID - 2125737 [pii]
AID - 10.1080/19490976.2022.2125737 [doi]
PST - ppublish
SO  - Gut Microbes. 2022 Jan-Dec;14(1):2125737. doi: 10.1080/19490976.2022.2125737.