PMID- 36175938
OWN - NLM
STAT- MEDLINE
DCOM- 20221003
LR  - 20221003
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 53
IP  - 1
DP  - 2022 Sep 30
TI  - Effects of porcine reproductive and respiratory syndrome virus (PRRSV) on thyroid 
      hormone metabolism in the late gestation fetus.
PG  - 74
LID - 10.1186/s13567-022-01092-3 [doi]
LID - 74
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) in late gestation 
      causes a profound suppression of circulating maternal and fetal thyroid hormone 
      during a critical window of development. To understand this relationship, we 
      evaluated thyroid hormone metabolism at the maternal-fetal interface and within 
      fetal tissues, along with hormone metabolite levels in serum. Fetuses were 
      classified using an established model based on viral load in serum and thymus, 
      and preservation status, including uninfected (UNIF), high-viral load viable 
      (HV-VIA), and high-viral load meconium-stained (HV-MEC), with additional controls 
      from sham-inoculated gilts (CON). Expression of three iodothyronine deiodinases, 
      five sulfotransferases, sulfatase, and two solute carriers known to transport 
      thyroid hormone were evaluated in maternal endometrium and fetal placenta, liver, 
      and kidney. Serum thyroxin (T4), reverse triiodothyronine (rT3), and 
      diiodothyronine (T2) were evaluated via liquid chromatography tandem mass 
      spectrometry. Significant changes in gene expression were observed in all four 
      tissues, with the liver being the most severely impacted. We observed local and 
      fetal specific regulation of maternal tissues through significant upregulation of 
      DIO2 and DIO3 expression in the endometrium corresponding to infected but viable 
      fetuses relative to uninfected and control fetuses. Expression levels of DIO2 and 
      DIO3 were significantly higher in the resilient (HV-VIA) fetuses relative to the 
      susceptible (HV-MEC) fetuses. A substantial decrease in serum T4 was confirmed, 
      with no corresponding increase in rT3 or T2. Collectively, these results show 
      that thyroid hormone metabolism is altered at the maternal-fetal interface and 
      within the PRRSV infected fetus and is associated with fetal viability.
CI  - (c) 2022. The Author(s).
FAU - Ison, Erin K
AU  - Ison EK
AD  - Department of Animal Science, Purdue University, West Lafayette, IN, 47906, USA.
FAU - Hopf-Jannasch, Amber S
AU  - Hopf-Jannasch AS
AD  - Bindley Bioscience Center, Purdue University, West Lafayette, IN, 47906, USA.
FAU - Harding, John C S
AU  - Harding JCS
AD  - Department of Large Animal Clinical Sciences, Western College of Veterinary 
      Medicine, University of Saskatchewan, 52 Campus Dr., Saskatoon, SK, S7N 5B4, 
      Canada.
FAU - Alex Pasternak, J
AU  - Alex Pasternak J
AUID- ORCID: 0000-0001-8596-3057
AD  - Department of Animal Science, Purdue University, West Lafayette, IN, 47906, USA. 
      jpastern@purdue.edu.
LA  - eng
GR  - ALGP47/Genome Alberta/
PT  - Journal Article
DEP - 20220930
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Diiodothyronines)
RN  - 5817-39-0 (Triiodothyronine, Reverse)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 3.1.6.- (Sulfatases)
RN  - Q51BO43MG4 (Thyroxine)
SB  - IM
MH  - Animals
MH  - Diiodothyronines
MH  - Female
MH  - Fetus
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Pregnancy
MH  - Sulfatases
MH  - Sulfotransferases
MH  - Sus scrofa
MH  - Swine
MH  - *Swine Diseases
MH  - Thyroxine
MH  - Triiodothyronine, Reverse
PMC - PMC9524047
OTO - NOTNLM
OT  - PRRS
OT  - fetal
OT  - host-pathogen interaction
OT  - hypothyroidism
OT  - maternal
OT  - thyroid hormone
COIS- The authors declare that they have no competing interest.
EDAT- 2022/09/30 06:00
MHDA- 2022/10/04 06:00
PMCR- 2022/09/30
CRDT- 2022/09/29 23:51
PHST- 2022/07/11 00:00 [received]
PHST- 2022/08/16 00:00 [accepted]
PHST- 2022/09/29 23:51 [entrez]
PHST- 2022/09/30 06:00 [pubmed]
PHST- 2022/10/04 06:00 [medline]
PHST- 2022/09/30 00:00 [pmc-release]
AID - 10.1186/s13567-022-01092-3 [pii]
AID - 1092 [pii]
AID - 10.1186/s13567-022-01092-3 [doi]
PST - epublish
SO  - Vet Res. 2022 Sep 30;53(1):74. doi: 10.1186/s13567-022-01092-3.