PMID- 36177471
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231101
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - Ruminal background of predisposed milk urea (MU) concentration in Holsteins.
PG  - 939711
LID - 10.3389/fmicb.2022.939711 [doi]
LID - 939711
AB  - Efforts to reduce nitrogen (N) emissions are currently based on the optimization 
      of dietary- N supply at average herd N requirements. The implementation of the 
      considerable individual differences and predispositions in N- use efficiency and 
      N- excretion in breeding programs is hampered by the difficulty of data 
      collection. Cow individual milk urea (MU) concentration has been proposed as an 
      easy-to-measure surrogate trait, but recent studies questioned its predictive 
      power. Therefore, a deeper understanding of the biological mechanisms underlying 
      predisposed higher (HMUg) or lower (LMUg) MU concentration in dairy cows is 
      needed. Considering the complex N- metabolism in ruminants, the distinction 
      between HMUg and LMUg could be based on differences in (i) the rumen microbial 
      community, (ii) the host-specific transcription processes in the rumen villi, and 
      (iii) the host-microbe interaction in the rumen. Therefore, rumen fluid and rumen 
      epithelial samples from 10 HMUg and 10 LMUg cows were analyzed by 16S sequencing 
      and HiSeq sequencing. In addition, the effect of dietary-N reduction on ruminal 
      shifts was investigated in a second step. In total, 10 differentially abundant 
      genera (DAG) were identified between HMUg and LMUg cows, elucidating greater 
      abundances of ureolytic Succinivibrionaceae_UCG-002 and 
      Ruminococcaceae_unclassified in LMUg animals and enhanced occurrences of 
      Butyvibrio in HMUg cows. Differential expression analysis revealed genes of the 
      bovine Major Histocompatibility Complex (BOLA genes) as well as MX1, ISG15, and 
      PRSS2 displaying candidates of MU predisposition that further attributed to 
      enhanced immune system activities in LMUg cows. A number of significant 
      correlations between microbial genera and host transcript abundances were 
      uncovered, including strikingly positive correlations of BOLA-DRA transcripts 
      with Roseburia and Lachnospiraceae family abundances that might constitute 
      particularly prominent microbial-host interplays of MU predisposition. The 
      reduction of feed-N was followed by 18 DAG in HMUg and 19 DAG in LMUg, depicting 
      pronounced interest on Shuttleworthia, which displayed controversial adaption in 
      HMUg and LMUg cows. Lowering feed-N further elicited massive downregulation of 
      immune response and energy metabolism pathways in LMUg. Considering breeding 
      selection strategies, this study attributed information content to MU about 
      predisposed ruminal N-utilization in Holstein-Friesians.
CI  - Copyright (c) 2022 Honerlagen, Reyer, Segelke, Muller, Prahl, Ponsuksili, 
      Trakooljul, Reinsch, Kuhla and Wimmers.
FAU - Honerlagen, Hanne
AU  - Honerlagen H
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 
      Dummerstorf, Germany.
FAU - Reyer, Henry
AU  - Reyer H
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 
      Dummerstorf, Germany.
FAU - Segelke, Dierck
AU  - Segelke D
AD  - IT-Solutions for Animal Production, Vereinigte Informationssysteme Tierhaltung 
      w.V. (vit), Verden, Germany.
FAU - Muller, Carolin Beatrix Maria
AU  - Muller CBM
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Nutritional 
      Physiology "Oskar Kellner", Dummerstorf, Germany.
FAU - Prahl, Marie Christin
AU  - Prahl MC
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Nutritional 
      Physiology "Oskar Kellner", Dummerstorf, Germany.
FAU - Ponsuksili, Siriluck
AU  - Ponsuksili S
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 
      Dummerstorf, Germany.
FAU - Trakooljul, Nares
AU  - Trakooljul N
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 
      Dummerstorf, Germany.
FAU - Reinsch, Norbert
AU  - Reinsch N
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Genetics and 
      Biometry, Dummerstorf, Germany.
FAU - Kuhla, Bjorn
AU  - Kuhla B
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Nutritional 
      Physiology "Oskar Kellner", Dummerstorf, Germany.
FAU - Wimmers, Klaus
AU  - Wimmers K
AD  - Research Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 
      Dummerstorf, Germany.
AD  - Faculty of Agricultural and Environmental Sciences, University of Rostock, 
      Rostock, Germany.
LA  - eng
PT  - Journal Article
DEP - 20220913
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9513179
OTO - NOTNLM
OT  - dairy cow
OT  - host gene expression
OT  - microbe-host relationship
OT  - milk urea
OT  - rumen microbiome
COIS- Author DS was employed by IT-Solutions for Animal Production, Vereinigte 
      Informationssysteme Tierhaltung w.V. (vit). The remaining authors declare that 
      the research was conducted in the absence of any commercial or financial 
      relationships that could be construed as a potential conflict of interest.
EDAT- 2022/10/01 06:00
MHDA- 2022/10/01 06:01
PMCR- 2022/09/13
CRDT- 2022/09/30 02:49
PHST- 2022/05/09 00:00 [received]
PHST- 2022/07/26 00:00 [accepted]
PHST- 2022/09/30 02:49 [entrez]
PHST- 2022/10/01 06:00 [pubmed]
PHST- 2022/10/01 06:01 [medline]
PHST- 2022/09/13 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.939711 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Sep 13;13:939711. doi: 10.3389/fmicb.2022.939711. 
      eCollection 2022.