PMID- 36178049
OWN - NLM
STAT- MEDLINE
DCOM- 20221024
LR  - 20221129
IS  - 1759-9679 (Electronic)
IS  - 1759-9660 (Linking)
VI  - 14
IP  - 40
DP  - 2022 Oct 20
TI  - Simultaneous and sensitive determination of the main metabolites of the 
      plasticizer DEHP and its substitutes DEHTP, DINCH and TEHTM in human urine by 
      coupling of on-line SPE, UHPLC and tandem mass spectrometry.
PG  - 3970-3981
LID - 10.1039/d2ay01293f [doi]
AB  - With the prominent but toxicologically critical plasticizer di-(2-ethylhexyl) 
      phthalate (DEHP) declining, alternative plasticizers are increasingly used 
      leading to a continuously more diverse exposure situation of humans with multiple 
      plasticizers. Therefore, an on-line SPE-LC-MS/MS method for the simultaneous 
      determination of the most relevant urinary biomarkers of exposure to DEHP and the 
      alternative plasticizers 1,2-cyclohexane dicarboxylic acid diisononyl ester 
      (DINCH), di-(2-ethylhexyl) terephthalate (DEHTP) and tri-(2-ethylhexyl) 
      trimellitate (TEHTM) was developed. The method is characterized by a high 
      sensitivity with limits of detection ranging from 0.006 to 0.047 mug L(-1) 
      combined with an easy and straightforward sample preparation procedure. The wide 
      linear working range of the method enables a reliable determination of analyte 
      background levels in the general population as well as its potential use for 
      monitoring studies investigating elevated plasticizer exposure settings. The 
      method was successfully applied to urine samples from ten volunteers without 
      occupational exposure to plasticizers revealing ubiquitous background exposure 
      levels of the common plasticizers DEHP, DEHTP and DINCH.
FAU - Kuhlmann, Laura
AU  - Kuhlmann L
AUID- ORCID: 0000-0002-5037-4399
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, Friedrich-Alexander-Universitat Erlangen-Nuremberg, Henkestr. 9-11, 
      91054 Erlangen, Germany. Elisabeth.Eckert@fau.de.
FAU - Munch, Frank
AU  - Munch F
AUID- ORCID: 0000-0002-2790-0418
AD  - Department of Pediatric Cardiac Surgery, University Hospital Erlangen, University 
      of Erlangen-Nuremberg, Germany.
FAU - Goen, Thomas
AU  - Goen T
AUID- ORCID: 0000-0002-8226-9227
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, Friedrich-Alexander-Universitat Erlangen-Nuremberg, Henkestr. 9-11, 
      91054 Erlangen, Germany. Elisabeth.Eckert@fau.de.
FAU - Eckert, Elisabeth
AU  - Eckert E
AUID- ORCID: 0000-0002-6037-9556
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, Friedrich-Alexander-Universitat Erlangen-Nuremberg, Henkestr. 9-11, 
      91054 Erlangen, Germany. Elisabeth.Eckert@fau.de.
LA  - eng
PT  - Journal Article
DEP - 20221020
PL  - England
TA  - Anal Methods
JT  - Analytical methods : advancing methods and applications
JID - 101519733
RN  - 0 (Plasticizers)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - 0 (Biomarkers)
RN  - 0 (Esters)
RN  - 0 (Cyclohexanes)
SB  - IM
MH  - Humans
MH  - *Plasticizers/analysis/metabolism
MH  - Tandem Mass Spectrometry/methods
MH  - Chromatography, Liquid/methods
MH  - *Diethylhexyl Phthalate/urine
MH  - Chromatography, High Pressure Liquid
MH  - Biomarkers
MH  - Esters
MH  - Cyclohexanes
EDAT- 2022/10/01 06:00
MHDA- 2022/10/25 06:00
CRDT- 2022/09/30 06:32
PHST- 2022/10/01 06:00 [pubmed]
PHST- 2022/10/25 06:00 [medline]
PHST- 2022/09/30 06:32 [entrez]
AID - 10.1039/d2ay01293f [doi]
PST - epublish
SO  - Anal Methods. 2022 Oct 20;14(40):3970-3981. doi: 10.1039/d2ay01293f.