PMID- 36181847
OWN - NLM
STAT- MEDLINE
DCOM- 20221026
LR  - 20221026
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 309
IP  - Pt 1
DP  - 2022 Dec
TI  - Short and long-term effects of nanobiomaterials in fish cell lines. Applicability 
      of RTgill-W1.
PG  - 136636
LID - S0045-6535(22)03129-0 [pii]
LID - 10.1016/j.chemosphere.2022.136636 [doi]
AB  - Nanobiomaterials (NBMs) are nanostructured materials for biomedical applications 
      that can reach aquatic organisms. The short and long-term effects of these 
      emerging contaminants are unknown in fish. The RTgill-W1 cell line has been 
      proposed as a model to predict the acute toxicity of chemicals to fish (OECD Test 
      Guideline n masculine 249). We assessed the applicability of this cell line to study the 
      short and long-term toxicity of 15 NBMs based on hydroxyapatites (HA), lipid 
      (LSNP/LNP), gold, iron oxide, carbon, poly l-Lactide acid (PLLA) fibers with Ag 
      and poly (lactide-co-glycolide) acid. Two more rainbow trout cell lines (RTL-W1, 
      from liver, and RTS-11, from spleen) were exposed, to identify possible 
      sensitivity differences among cells. Exposures to a range of concentrations 
      (0.78-100 mug/mL) lasted for 24 h. Additionally, the RTgill-W1 was used to perform 
      long-term (28 d exposure) and recovery (14 d exposure/14 d recovery) assays. 
      Cells were exposed to the 24 h-IC(20) and/or to 100 mug/mL. A triple cytotoxicity 
      assay was conducted. After 24 h, only PLLA Fibers-Ag showed cytotoxicity 
      (IC(50) < 100 mug/mL). However, the NBMs in general provoked 
      concentration-dependent effects after long-term exposures, except the LSNPs. A 
      recovery of viability was only observed for AuNPs, AuNRods, Fe(3)O(4)PEG-PLGA, 
      MgHA-Collag_Scaffolds, Ti-HA and TiHA-Alg NPs.These results evidenced the need to 
      test the long-term toxicity of NBMs and showed differences in cytotoxicity 
      probably associated to different mechanisms of toxic action. The RTgill-W1 was 
      useful to screen short and long-term toxicities of NBMs and appears as a 
      promiseful model to assess possible toxicity of NBMs in fish.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Hernandez-Moreno, D
AU  - Hernandez-Moreno D
AD  - National Institute of Agricultural and Food Research and Technology (INIA), 
      Spanish National Research Council (CSIC), Department of Environment and Agronomy, 
      Carretera de La Coruna Km 7, Madrid, Spain. Electronic address: 
      david.hernandez@inia.csic.es.
FAU - Navas, J M
AU  - Navas JM
AD  - National Institute of Agricultural and Food Research and Technology (INIA), 
      Spanish National Research Council (CSIC), Department of Environment and Agronomy, 
      Carretera de La Coruna Km 7, Madrid, Spain.
FAU - Fernandez-Cruz, M L
AU  - Fernandez-Cruz ML
AD  - National Institute of Agricultural and Food Research and Technology (INIA), 
      Spanish National Research Council (CSIC), Department of Environment and Agronomy, 
      Carretera de La Coruna Km 7, Madrid, Spain. Electronic address: 
      fcruz@inia.csic.es.
LA  - eng
PT  - Journal Article
DEP - 20220928
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 7440-57-5 (Gold)
RN  - 7440-44-0 (Carbon)
RN  - 0 (Hydroxyapatites)
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Gold/metabolism
MH  - *Metal Nanoparticles
MH  - Cell Line
MH  - *Oncorhynchus mykiss/metabolism
MH  - Carbon/metabolism
MH  - Hydroxyapatites/metabolism
MH  - Lipids
OTO - NOTNLM
OT  - Cytotoxicity
OT  - In vitro
OT  - Long-term
OT  - Nanobiomaterials
OT  - Recovery assay
OT  - Short-term
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/10/02 06:00
MHDA- 2022/10/27 06:00
CRDT- 2022/10/01 19:33
PHST- 2022/04/29 00:00 [received]
PHST- 2022/09/12 00:00 [revised]
PHST- 2022/09/25 00:00 [accepted]
PHST- 2022/10/02 06:00 [pubmed]
PHST- 2022/10/27 06:00 [medline]
PHST- 2022/10/01 19:33 [entrez]
AID - S0045-6535(22)03129-0 [pii]
AID - 10.1016/j.chemosphere.2022.136636 [doi]
PST - ppublish
SO  - Chemosphere. 2022 Dec;309(Pt 1):136636. doi: 10.1016/j.chemosphere.2022.136636. 
      Epub 2022 Sep 28.