PMID- 36182800 OWN - NLM STAT- MEDLINE DCOM- 20221017 LR - 20221017 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 245 DP - 2022 Oct 15 TI - Association between prenatal exposure to perfluoroalkyl substances and anogenital distance in female neonates. PG - 114130 LID - S0147-6513(22)00970-8 [pii] LID - 10.1016/j.ecoenv.2022.114130 [doi] AB - BACKGROUND: Perfluoroalkyl substances (PFASs) have been reported to exert reproductive toxicity. Anogenital distance (AGD) is a biomarker of intrauterine androgen exposure and an indicator of genital development. An animal study reported that female neonatal rats exposed to perfluorooctanoic acid or perfluorooctane sulfonate (PFOS) during postnatal days 1-5 exhibited a longer AGD, while epidemiological studies have shown inconsistent results. This study aimed to examine the effects of prenatal exposure to PFASs on the AGD in female neonates. METHODS: PFAS levels were measured in plasma samples obtained from pregnant women at 12-16 gestational weeks using high-performance liquid chromatography/mass spectrometry. The AGD of each female neonate was measured within 3 days after delivery. The anogenital index (AGI), calculated as AGD divided by weight, was also determined. A total of 362 motherinfant pairs were included in this study. A multivariate linear regression model was used to examine the association between prenatal ln-transformed concentrations of PFASs and AGD/AGI. In addition, weighted quantile sum regression (WQSR) and Bayesian kernel machine regression (BKMR) models were used to assess the overall effects of a mixture of PFASs on the AGD/AGI and to identify important contributors to the overall effect. RESULTS: There was a consistent pattern of association between maternal PFAS concentrations and increased AGDanus to posterior fourchette (AF), AGDanus to clitoris (AC), and AGIAF lengths at birth. Statistical significance was found between maternal ln-transformed concentrations of perfluorohexane sulfonate (PFHxS), perfluorododecanoic acid, and perfluorotridecanoic acid and AGDAF, with beta values (95% confidence interval [CI]) of 0.83 (0.16, 1.51), 0.32 (0.05, 0.59), and 0.25 (0.00, 0.51) mm, respectively; between PFOS and AGDAC, with a beta value (95% CI) of 0.63 (0.04, 1.21) mm; and between PFHxS and AGIAF, with a beta value (95% CI) of 0.22 (0.02, 0.43) mm/kg. Similarly, the WQSR and BKMR models showed that an increase in the AGDAF/AGIAF at birth was associated with co-exposure to a mixture of PFASs. CONCLUSION: High maternal concentrations of PFASs were associated with increased AGD in female neonates, indicating that PFASs may impair reproductive development in female offspring in early life. CI - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Li, Jincan AU - Li J AD - National Health Commission Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Public Health, Fudan University, Shanghai 200237, China. FAU - Yang, Limei AU - Yang L AD - The First People's Hospital of Jiashan, Jiaxing Zhejiang Province 314199, China. FAU - He, Gengsheng AU - He G AD - School of Public Health/Key Laboratory of Public Health Safety of Ministry of Education, Fudan University, Shanghai 200032, China. FAU - Wang, Binbin AU - Wang B AD - Center for Genetics, National Research Institute for Family Planning, Beijing 100081, China. FAU - Miao, Maohua AU - Miao M AD - National Health Commission Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Public Health, Fudan University, Shanghai 200237, China. FAU - Ji, Honglei AU - Ji H AD - National Health Commission Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Public Health, Fudan University, Shanghai 200237, China. FAU - Wen, Sheng AU - Wen S AD - Hubei Provincial Key Laboratory of Applied Toxicology, National Reference Laboratory of Dioxin, Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei 430079, China. FAU - Cao, Wencheng AU - Cao W AD - Hubei Provincial Key Laboratory of Applied Toxicology, National Reference Laboratory of Dioxin, Hubei Provincial Center for Disease Control and Prevention, Wuhan, Hubei 430079, China. FAU - Yuan, Wei AU - Yuan W AD - National Health Commission Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Public Health, Fudan University, Shanghai 200237, China. FAU - Liang, Hong AU - Liang H AD - National Health Commission Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Public Health, Fudan University, Shanghai 200237, China. Electronic address: lianghong@sibpt.com. LA - eng PT - Journal Article DEP - 20220928 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 RN - 0 (Alkanesulfonic Acids) RN - 0 (Androgens) RN - 0 (Biomarkers) RN - 0 (Environmental Pollutants) RN - 0 (Fluorocarbons) RN - 9H2MAI21CL (perfluorooctane sulfonic acid) RN - FX3WJ41CMX (perflexane) SB - IM MH - *Alkanesulfonic Acids/toxicity MH - Androgens MH - Animals MH - Bayes Theorem MH - Biomarkers MH - *Environmental Pollutants/toxicity MH - Female MH - *Fluorocarbons/toxicity MH - Humans MH - Maternal Exposure/adverse effects MH - Pregnancy MH - *Prenatal Exposure Delayed Effects MH - Rats OTO - NOTNLM OT - Anogenital distance OT - Genital development OT - Maternal exposure OT - Perfluoroalkyl substances OT - Prospective study COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2022/10/02 06:00 MHDA- 2022/10/18 06:00 CRDT- 2022/10/01 23:12 PHST- 2022/04/28 00:00 [received] PHST- 2022/09/25 00:00 [revised] PHST- 2022/09/26 00:00 [accepted] PHST- 2022/10/02 06:00 [pubmed] PHST- 2022/10/18 06:00 [medline] PHST- 2022/10/01 23:12 [entrez] AID - S0147-6513(22)00970-8 [pii] AID - 10.1016/j.ecoenv.2022.114130 [doi] PST - ppublish SO - Ecotoxicol Environ Saf. 2022 Oct 15;245:114130. doi: 10.1016/j.ecoenv.2022.114130. Epub 2022 Sep 28.